Anti-Human CD45RO Antibody, Clone UCHL1

Mouse monoclonal IgG2a antibody against human, chimpanzee, common marmoset CD45RO
From: 110 USD

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Mouse monoclonal IgG2a antibody against human, chimpanzee, common marmoset CD45RO
From: 110 USD

Overview

The UCHL1 antibody reacts with an extracellular epitope on CD45RO, which is the shortest isoform of CD45, a type I transmembrane glycoprotein and member of the protein tyrosine phosphatase family (receptor class 1/6 subfamily). Alternative splicing of exons 4, 5 and 6 that encode the extracellular RA, RB and RC polypeptides of CD45 gives rise to up to 8 isoforms with molecular masses of 180 - 240 kDa. Excision of all three exons generates the ~180 kDa CD45RO isoform, which is expressed on activated and memory (but not naïve) T cells, some B cell subsets, activated monocytes and macrophages, and granulocytes. CD45RO enhances both T cell receptor- and B cell receptor-mediated activation and is a known ligand for CD22 on B cells. The UCHL1 antibody has been used to identify T cell lymphomas and leukemia and is commonly used in combination with antibodies against CD45RA to discern memory and naïve T cells. The proportion of CD45RO+ (memory) T cells typically increases with age. The UCHL1 epitope is destroyed by treatment with neuraminidase or O-glycosidase.
Subtype:
Primary Antibodies
Target Antigen:
CD45RO
Alternative Names:
B220, CD45, GP180, LCA, L-CA, LY5, Protein tyrosine phosphatase receptor type C, PTPRC, T200
Reactive Species:
Common Marmoset; Chimpanzee; Human
Conjugation:
Alexa Fluor 488; APC; Biotin; FITC; PE; PerCP-Cyanine5.5; Unconjugated
Host Species:
Mouse
Cell Type:
T Cells; T Cells, CD4+; T Cells, CD8+
Application:
Cell Isolation; Electron Microscopy; ELISA; Flow Cytometry; Functional Assay; Immunocytochemistry; Immunofluorescence; Immunohistochemistry; Immunoprecipitation; Western Blotting
Area of Interest:
Immunology; Stem Cell Biology
Clone:
UCHL1
Gene ID:
5788
Isotype:
IgG2a, kappa

Scientific Resources

Educational Materials

(4)

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Data for Unconjugated

Figure 1. Data for Unconjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD45RO Antibody, Clone UCHL1, followed by a rat anti-mouse IgG2a antibody, PE (filled histogram), or Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173 (Catalog #60071), followed by a rat anti-mouse IgG2a antibody, PE (solid line histogram). (B) Flow cytometry analysis of human PBMCs (gated on lymphocytes) labeled with Anti-Human CD45RO Antibody, Clone UCHL1, followed by a rat anti­mouse IgG2a antibody, PE and an anti-human CD45RA antibody, FITC. (C) Flow cytometry analysis of human PBMCs (gated on lymphocytes) and labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC­173, followed by a rat anti-mouse IgG2a antibody, PE and an anti-human CD45RA antibody, FITC.

Data for Alexa Fluor® 488-Conjugated

Figure 2. Data for Alexa Fluor® 488-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs; gated on CD3+ cells) labeled with Anti-Human CD45RO Antibody, Clone UCHL1, Alexa Fluor® 488 and an anti-human CD45RA antibody, APC. (B) Flow cytometry analysis of human PBMCs (gated on CD3+ cells) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, Alexa Fluor® 488 (Catalog #60071AD) and an anti-human CD45RA antibody, APC.

Data for APC-Conjugated

Figure 3. Data for APC-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMSs) labeled with Anti-Human CD45RO Antibody, Clone UCHL1, APC (filled histogram) or Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, APC (Catalog #60071AZ) (solid line histogram) (B) Flow cytometry analysis of human PBMCs (gated on CD3+ cells) labeled with Anti-Human CD45RO Antibody, Clone UCHL1, APC and an anti-human CD45RA antibody, FITC. (C) Flow cytometry analysis of human PBMCs (gated on CD3+ cells) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, APC and an anti-human CD45RA antibody, FITC.

Data for Biotin-Conjugated

Figure 4. Data for Biotin-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs; gated on CD3+ cells) labeled with Anti-Human CD45RO Antibody, Clone UCHL1, Biotin, followed by streptavidin (SAV) APC and an anti-human CD45RA antibody, FITC (B) Flow cytometry analysis of human PBMCs (gated on CD3+ cells) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, Biotin (Catalog #60071BT), followed by SAV APC and an anti-human CD45RA antibody, FITC.

Data for FITC-Conjugated

Figure 5. Data for FITC-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs; gated on CD3+ cells) labeled with Anti-Human CD45RO Antibody, Clone UCHL1, FITC and an anti-human CD45RA antibody, APC. (B) Flow cytometry analysis of human PBMCs (gated on CD3+ cells) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, FITC (Catalog #60071FI) and an anti-human CD45RA antibody, APC.

Data for PE-Conjugated

Figure 6. Data for PE-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD45RO Antibody, Clone UCHL1, PE (filled histogram) or Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, PE (Catalog #60071PE) (solid line histogram). (B) Flow cytometry analysis of human PBMCs (gated on CD3+ cells) labeled with Anti-Human CD45RO Antibody, Clone UCHL1, PE and an anti-human CD45RA antibody, APC. (C) Flow cytometry analysis of human PBMCs (gated on CD3+ cells) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, PE and an anti-human CD45RA antibody, APC.

Data for PerCP-Cy55-Conjugated

Figure 7. Data for PerCP-Cy55-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD45RO Antibody, Clone UCHL1, PerCP-Cy5.5 and an anti-human CD45RA antibody, FITC. Cells were gated on CD3+ cells (T lymphocytes).
(B) Flow cytometry analysis of human PBMCs labeled with a mouse IgG2a, kappa isotype control antibody, PerCP-Cy5.5 and an anti-human CD45RA antibody, FITC. Cells were gated on CD3+ cells (T lymphocytes).

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.