Anti-Human CD16 Antibody, Clone 3G8

Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD16

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From: 110 USD

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Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD16
From: 110 USD

Overview Related Products Scientific Resources Product Applications Data and Publications

Overview

The 3G8 antibody reacts with CD16 (FcγRIII or low affinity IgG receptor III), a type 1 transmembrane glycoprotein belonging to the Ig superfamily. CD16 exists in two distinct isoforms: CD16a, a 50 - 65 kDa form expressed on NK cells, activated monocytes, macrophages, and placental trophoblasts, and CD16b, an ~48 kDa glycosylphosphatidylinositol (GPI)-anchored form expressed on neutrophils, basophils and eosinophils and found as at least two polymorphic variants, termed NA1 and NA2. CD16 binds weakly to the Fc region of monomeric, aggregated or complexed IgG, particularly the IgG1 and IgG3 isotypes. Binding of IgG to either CD16 isoform induces signaling pathways that modulate several types of responses, including antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, cytokine release and proliferation. CD16/IgG interactions can result in non-specific labeling in antibody-based detection and cell separation experiments and the 3G8 antibody may be employed as a blocking antibody to reduce non-specific binding.
Subtype:
Primary Antibodies
Target Antigen:
CD16
Alternative Names:
CD16A, CD16B, Fc-gamma RIII; FCG3; FCGR3; FCGRIII; FcγRIII; FCR-10, FcRIII, IGFR3, IMD20
Reactive Species:
Human; Rhesus; Cynomolgus; Baboon; Capuchin Monkey; Chimpanzee; Common Marmoset; Cotton-Topped Tamarin; Macaque; Sooty Mangabey; Squirrel Monkey
Conjugation:
Alexa Fluor 488; APC; Biotin; FITC; PE; Unconjugated
Host Species:
Mouse
Cell Type:
Granulocytes and Subsets; Monocytes
Application:
Cell Isolation; CyTOF; ELISA; Flow Cytometry; Functional Assay; Immunocytochemistry; Immunofluorescence; Immunohistochemistry; Immunoprecipitation; Neutralization and Blocking; Depletion
Area of Interest:
Immunology
Clone:
3G8
Gene ID:
2214
Isotype:
IgG1, kappa

Scientific Resources

Product Documentation

Educational Materials

(5)
Brochure
Hematopoietic Stem and Progenitor Cells - Products for Your Research
Brochure
Complete Antibody Listing
Brochure
Tools For Your Immunology Research
Brochure
Immunology Antibodies
Wallchart
Human Immune Cytokines

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area Workflow Stages for
Workflow Stages
Granulocyte Research
Cell Sourcing
Cell Isolation
Cell Characterization
Hematopoietic Stem and Progenitor Cell Research
Cell Sourcing and Isolation
Expansion and Differentiation
Analysis
Monocyte Research
Cell Sourcing
Cell Isolation
Differentiation and Maturation
Cell Characterization

Data and Publications

Data

Data for Unconjugated

Figure 1. Data for Unconjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD16 Antibody, Clone 3G8, followed by a rat anti-mouse IgG1 antibody, PE (filled histogram), or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21 (Catalog #60070), followed by a rat anti-mouse IgG1 antibody, PE (solid line histogram).

Data for Alexa Fluor® 488-Conjugated

Figure 2. Data for Alexa Fluor® 488-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD16 Antibody, Clone 3G8, Alexa Fluor® 488 (filled histogram) or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, Alexa Fluor® 488 (Catalog #60070AD) (solid line histogram).

Data for APC-Conjugated

Figure 3. Data for APC-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD16 Antibody, Clone 3G8, APC (filled histogram) or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, APC (Catalog #60070AZ) (solid line histogram).

Data for Biotin-Conjugated

Figure 4. Data for Biotin-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD16 Antibody, Clone 3G8, Biotin, followed by streptavidin (SAV) APC (filled histogram), or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, Biotin (Catalog #60070BT), followed by SAV APC (solid line histogram).

Data for FITC-Conjugated

Figure 5. Data for FITC-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD16 Antibody, Clone 3G8, FITC (filled histogram) or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, FITC (Catalog #60070FI) (solid line histogram).

Data for PE-Conjugated

Figure 6. Data for PE-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD16 Antibody, Clone 3G8, PE (filled histogram) or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, PE (Catalog #60070PE) (solid line histogram).

Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.