Anti-Human CD14 Antibody, Clone M5E2

Mouse monoclonal IgG2a antibody against human, rhesus, cynomolgus CD14
From: 106 USD

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Mouse monoclonal IgG2a antibody against human, rhesus, cynomolgus CD14
From: 106 USD

Overview

The M5E2 antibody reacts with CD14, an ~53 - 55 kDa GPI-anchored transmembrane glycoprotein expressed at high levels on the surface of peripheral blood monocytes and macrophages, and at lower levels on granulocytes. An ~10-fold difference in expression levels between monocytes/macrophages and granulocytes makes CD14 a useful marker for distinguishing these cell populations. CD14 is also found on tissue macrophages, Langerhans cells and dendritic cells. CD14 functions as a high-affinity receptor for complexes of lipopolysaccharide (LPS) and serum LPS-binding protein and modulates LPS-dependent signal transduction during the immune response to gram-negative pathogens by acting as a co-receptor for TLR 4 and MD-2. This triggers activation of NF-kappa-B, cytokine secretion, and induction of the inflammatory response. Two soluble forms of CD14 have also been described (~48 and ~55 kDa).

This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Human Myeloid Positive Selection Kit (Catalog #18653) and EasySep™ Human CD14 Positive Selection Kit (Catalog #18058); partial blocking may be observed, as well as EasySep™ HLA Whole Blood CD33 Positive Selection Kit (Catalog #18287HLA), EasySep™ HLA Whole Blood Myeloid Positive Selection Kit (Catalog #18683HLA), and EasySep™ Human Buffy Coat CD14 Positive Selection Kit (Catalog #18088).
Subtype:
Primary Antibodies
Target Antigen:
CD14
Alternative Names:
LPS receptor
Reactive Species:
Capuchin Monkey; Common Marmoset; Cotton-Topped Tamarin; Cynomolgus; Rhesus; Squirrel Monkey; Chimpanzee; Cow; Dog; Human; Macaque; Pig; Sheep
Conjugation:
Alexa Fluor 488; APC; Biotin; FITC; PE; Unconjugated
Host Species:
Mouse
Cell Type:
Monocytes
Application:
CyTOF; Flow Cytometry; Functional Assay; Immunocytochemistry; Immunofluorescence; Immunohistochemistry; Neutralization and Blocking
Area of Interest:
Immunology
Clone:
M5E2
Gene ID:
929
Isotype:
IgG2a, kappa

Scientific Resources

Educational Materials

(6)

Data and Publications

Data

Data for Alexa Fluor® 488-Conjugated

Figure 1. Data for Alexa Fluor® 488-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD14 Antibody, Clone M5E2, Alexa Fluor® 488 and Anti-Human CD45 Antibody, Clone HI30, APC (Catalog #60018AD). (B) Flow cytometry analysis of human PBMCs processed with the EasySep™ Human CD14 Positive Selection Kit and labeled with Anti-Human CD14 Antibody, Clone M5E2, Alexa Fluor® 488. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, Alexa Fluor® 488 (Catalog #60071AD) is shown (solid line histogram). (C) Flow cytometry analysis of human whole blood nucleated cells processed with the EasySep™ HLA Whole Blood CD33 Positive Selection Kit and labeled with Anti-Human CD14 Antibody, Clone M5E2, Alexa Fluor® 488. Histograms show labeling of whole blood nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, Alexa Fluor® 488 is shown (solid line histogram).

Data for APC-Conjugated

Figure 2. Data for APC-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD14 Antibody, Clone M5E2, APC (filled histogram) or Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, APC (Catalog #60071AZ; solid line histogram). (B) Flow cytometry analysis of human PBMCs processed with the EasySep™ Human Myeloid Positive Selection Kit and labeled with Anti-Human CD14 Antibody, Clone M5E2, APC. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, APC is shown (solid line histogram). (C) Flow cytometry analysis of human whole blood nucleated cells processed with the EasySep™ HLA Whole Blood CD33 Positive Selection Kit and labeled with Anti-Human CD14 Antibody, Clone M5E2, APC. Histograms show labeling of whole blood nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, APC is shown (solid line histogram).

Data for Biotin-Conjugated

Figure 3. Data for Biotin-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD14 Antibody, Clone M5E2, Biotin followed by streptavidin (SAV) APC (filled histogram), or Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, Biotin (Catalog #60071BT), followed by SAV APC (solid line histogram). (B) Flow cytometry analysis of human PBMCs processed with the EasySep™ Human Myeloid Positive Selection Kit and labeled with Anti-Human CD14 Antibody, Clone M5E2, Biotin followed by streptavidin (SAV) APC. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, Biotin followed by SAV APC is shown (solid line histogram). (C) Flow cytometry analysis of human whole blood nucleated cells processed with the EasySep™ HLA Whole Blood CD33 Positive Selection Kit and labeled with Anti-Human CD14 Antibody, Clone M5E2, Biotin followed by streptavidin (SAV) APC. Histograms show labeling of whole blood nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, Biotin followed by SAV APC is shown (solid line histogram).

Data for FITC-Conjugated

Figure 4. Data for FITC-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD14 Antibody, Clone M5E2, FITC (filled histogram), or Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, FITC (Catalog #60071FI; solid line histogram). (B) Flow cytometry analysis of human PBMCs processed with the EasySep™ Human Myeloid Positive Selection Kit and labeled with Anti-Human CD14 Antibody, Clone M5E2, FITC. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, FITC is shown (solid line historgram). (C) Flow cytometry analysis of human whole blood nucleated cells processed with the EasySep™ HLA Whole Blood CD33 Positive Selection Kit and labeled with Anti-Human CD14 Antibody, Clone M5E2, FITC. Histograms show labeling of whole blood nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, FITC is shown (solid line histogram).

Data for PE-Conjugated

Figure 5. Data for PE-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD14 Antibody, Clone M5E2, PE (filled histogram), or Mouse IgG2a, kappa Isotype Control Antibody, Clone HI30, APC (Catalog #60018AZ). (B) Flow cytometry analysis of human PBMCs processed with the EasySep™ Human CD14 Positive Selection Kit and labeled with Anti-Human CD14 Antibody, Clone M5E2, PE. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, PE (Catalog #60071PE) is shown (solid line historgram). (C) Flow cytometry analysis of human whole blood nucleated cells processed with the EasySep™ HLA Whole Blood CD33 Positive Selection Kit and labeled with Anti-Human CD14 Antibody, Clone M5E2, FITC. Histograms show labeling of whole blood nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, PE is shown (solid line histogram).

Data for Unconjugated

Figure 6. Data for Unconjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD14 Antibody, Clone M5E2, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI) (filled histogram), or Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173 (Catalog #60071), followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (solid line histogram).

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