Anti-Mouse CD25 Antibody, Clone 3C7
Overview
This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Mouse T Cell Enrichment Kit (Catalog #19751).
Related Products
Scientific Resources
Product Documentation
| Document Type | Product Name | Catalog # | Lot # | Language |
|---|---|---|---|---|
|
Document Type
Product Information Sheet
|
Product Name
Anti-Mouse CD25 Antibody, Clone 3C7
|
Catalog # 60010 |
Lot # All |
Language English |
|
Document Type
Product Information Sheet
|
Product Name
Anti-Mouse CD25 Antibody, Clone 3C7, FITC
|
Catalog # 60010FI, 60010FI.1 |
Lot # All |
Language English |
|
Document Type
Product Information Sheet
|
Product Name
Anti-Mouse CD25 Antibody, Clone 3C7, PE
|
Catalog # 60010PE, 60010PE.1 |
Lot # All |
Language English |
|
Document Type
Safety Data Sheet
|
Product Name
Anti-Mouse CD25 Antibody, Clone 3C7
|
Catalog # 60010 |
Lot # All |
Language English |
|
Document Type
Safety Data Sheet
|
Product Name
Anti-Mouse CD25 Antibody, Clone 3C7, FITC
|
Catalog # 60010FI, 60010FI.1 |
Lot # All |
Language English |
|
Document Type
Safety Data Sheet
|
Product Name
Anti-Mouse CD25 Antibody, Clone 3C7, PE
|
Catalog # 60010PE, 60010PE.1 |
Lot # All |
Language English |
Educational Materials(2)
Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Data and Publications
Data
Figure 1. Data for Unconjugated
(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on CD3e+ cells) labeled with Anti-Mouse CD25 Antibody, Clone 3C7, followed by a mouse anti-rat IgG2b antibody, FITC and Anti-Mouse CD4, Clone RM4-5, PE (Catalog #60017PE). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on CD3e+ cells) labeled with Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530 (Catalog #60077), followed by a mouse anti-rat IgG2b antibody, FITC and Anti-Mouse CD4, Clone RM4-5, PE.
Figure 2. Data for FITC-Conjugated
(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD25 Antibody, Clone 3C7, FITC and anti-mouse CD3 APC.
(B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with a rat IgG2b, kappa FITC isotype control antibody and anti-mouse CD3 APC.
(C) Flow cytometry analysis of mouse T cells activated with antibodies against CD3 and CD28. C57BL/6 mouse splenocytes were processed with the EasySep™ Mouse T Cell Enrichment Kit and the enriched T cells were cultured in the absence (Unstimulated) or presence (Stimulated) of Anti-Mouse CD28 and plate-bound Anti-Mouse CD3e for 48 hours, then labeled with Anti-Mouse CD25 Antibody, Clone 3C7, FITC and analyzed for CD25 expression. Upregulation of CD25 following stimulation with CD3 and CD28 is apparent (Stimulated). Labeling of C57BL/6 mouse splenocytes with a rat IgG2b, kappa Alexa Fluor® 488 isotype control antibody is shown (open histogram).
Figure 3. Data for PE-Conjugated
(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD25 Antibody, Clone 3C7, PE and anti-mouse CD3 APC.
(B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with a rat IgG2b, kappa PE isotype control antibody and anti-mouse CD3 APC.
(C) Flow cytometry analysis of mouse T cells activated with antibodies against CD3 and CD28. C57BL/6 mouse splenocytes were processed with the EasySep™ Mouse T Cell Enrichment Kit (Catalog #19751) and the enriched T cells were cultured in the absence (Unstimulated) or presence (Stimulated) of Anti-Mouse CD28 and plate-bound Anti-Mouse CD3e for 48 hours, then labeled with Anti-Mouse CD25 Antibody, Clone 3C7, PE and analyzed for CD25 expression. Upregulation of CD25 following stimulation with CD3 and CD28 is apparent (Stimulated). Labeling of C57BL/6 mouse splenocytes with a rat IgG2b, kappa Alexa Fluor® 488 isotype control antibody is shown (open histogram).
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
