MSC Characterization Antibody Panel

Multi-color flow cytometry kit for characterization of human mesenchymal stromal cells
MSC Characterization Antibody Panel

Multi-color flow cytometry kit for characterization of human mesenchymal stromal cells

1 Kit
Catalog # 100-0354
536 USD

Overview

The MSC Characterization Antibody Panel is designed for the phenotypic analysis of human mesenchymal stromal cells (MSCs). This kit contains three fluorochrome-conjugated antibodies for the identification of human MSCs, and a fluorochrome-conjugated antibody for the identification of non-MSCs. When measured by flow cytometry, ≥ 95% of the MSC population must express CD73, CD90, and CD105, and these cells must lack expression (≤ 2% positive) of CD45. These reagents provide single-step labeling for the verification of human MSCs.The MSC Characterization Antibody Panel can be used to identify human MSCs derived from various tissues such as bone marrow MSCs, pluripotent stem cell-derived mesenchymal progenitor cells, adipose-derived MSCs, dental pulp-derived MSCs, and umbilical cord-derived MSCs.
Subtype
Primary Antibodies
Cell Type
Mesenchymal Stromal Cells
Application
Flow Cytometry
Area of Interest
Endothelial Cell Biology, Stem Cell Biology

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
MSC Characterization Antibody Panel
Catalog #
100-0354
Lot #
All
Language
English

Educational Materials (2)

Brochure
Reliable Antibodies for Your Research
Brochure
MesenCult™-ACF Plus

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Flow cytometry dot plots comparing the difference in expression of CD45, CD73, CD90, and CD105 in human bone marrow-derived mesenchymal stromal cells (MSCs) labeled with the hMSC CD45/CD73/CD90/CD105 Antibody Panel and cultured either in MesenCult™-ACF Plus Medium or in serum-containing medium.

Figure 1. Flow Cytometry Analysis of Human Bone Marrow-Derived Mesenchymal Stromal Cells Labeled Using the hMSC CD45/CD73/CD90/CD105 Antibody Panel

(A) When cultured in MesenCult™-ACF Plus Medium, the labeled human bone marrow (BM)-derived mesenchymal stromal cells (MSCs), demonstrated positive expression of CD73, CD105, and CD90 as well as negative expression of CD45 at P0. (B) When cultured in serum-containing medium, the BM-derived MSCs exhibit reduced CD73, CD105, and CD90 expression as well as increased CD45 expression at P0 due to contaminating CD45+ cells.

Contact STEMCELL Technologies

Our Customer Service, Sales, and Product and Scientific Support departments in North America are available between 6 am and 5 pm Pacific Time (9 am and 8 pm Eastern Time). One of our representatives will be happy to help you by telephone or email. Please complete the form to contact us by email. A representative will get back to you shortly.
  •  

StemCell Technologies Inc. and affiliates ("STEMCELL Technologies") does not share your email address with third parties. StemCell Technologies Inc. will use your email address to confirm your identity and send you newsletters, transaction-related emails, promotional and customer service emails in accordance with our privacy policy. You can change your email preferences at any time.