EasySep™ Mouse Pan-ILC Enrichment Kit

16-Minute cell isolation using Immunomagnetic negative selection
EasySep™ Mouse Pan-ILC Enrichment Kit

Immunomagnetic enrichment of untouched mouse ILC1, ILC2, and ILC3s

1 x 10^9 cells
Catalog # 19875
721 USD
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Required Products
  1. EasySep™ Magnet
    EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer

Overview

This product is designed to enrich group 1, 2 and 3 innate lymphoid cells (ILC1, ILC2 and ILC3s) from single-cell suspensions of mouse tissues by negative selection. Unwanted cells are targeted for removal with biotinylated antibodies directed against non-ILCs and streptavidin-coated magnetic particles (RapidSpheres™). Labeled cells are separated using an EasySep™ magnet without the use of columns. Unwanted cells remain in the tube as desired cells are simply poured off into a new tube. Isolated cells are immediately available for downstream applications such as flow cytometry and cell sorting.
Advantages
• Fast, easy-to-use and column-free
• Isolated cells are untouched
• Facilitates rapid flow sorting of ILCs
Components
  • EasySep™ Mouse Pan-ILC Enrichment Kit (Catalog #19875)
    • EasySep™ Mouse Pan-ILC Enrichment Cocktail, 0.5 mL
    • EasySep™ Streptavidin RapidSpheres™ 50001, 1.0 mL
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• EasyPlate™ (Catalog #18102)
Subtype
Cell Isolation Kits
Cell Type
Innate Lymphoid Cells
Species
Mouse
Sample Source
Bone Marrow, Lung, Other
Selection Method
Negative
Application
Cell Isolation
Brand
EasySep
Area of Interest
Immunology

Related Products

Labeling Antibodies

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
EasySep™ Mouse Pan-ILC Enrichment Kit
Catalog #
19875
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
EasySep™ Mouse Pan-ILC Enrichment Kit
Catalog #
19875
Lot #
All
Language
English

Educational Materials(9)

Brochure
EasySep™ Cell Separation Technology
Brochure
Tools For Your Immunology Research
Brochure
Cell Enrichment Prior to Cell Sorting
Wallchart
Innate Lymphoid Cells
Wallchart
Frequencies and Percentages of Mouse Immune Cell Types
Video
0:01
EasySep™ Mouse Cell Isolation Kits: How Much Time Will You Save?
Video
1:57
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
Video
1:13
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
Video
0:57
Simultaneous Cell Isolation from Multiple Samples Using the EasyEights™ EasySep™ Magnet

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Figure 1. Typical EasySep™ Mouse Pan-ILC Enrichment Profile

Starting with a naïve mouse lung single-cell suspension, the total ILC content (CD45+Lin-CD127+) of the enriched fraction typically ranges from 3.1 - 7.6%. In the above example, the percentages of ILCs in the start and final enriched fractions are 0.4% and 3.9% (or 0.4% and 6.8% of CD45+ cells), respectively. NOTE: The ILC content of the start fraction typically ranges from 0.3 - 0.6%.

Figure 2. Typical EasySep™ Mouse Pan-ILC Enrichment Profile from Mouse Lymph Node

Starting with a naïve mouse lymph node single-cell suspension, the total ILC content (CD45+Lin-CD127+) of the enriched fraction typically ranges from 21.1 - 45.2%. In the above example, the percentages of ILCs in the start and final enriched fractions are 0.3% and 17.6% (or 0.3% and 24.7% of CD45+ cells), respectively. NOTE: The ILC content of the start fraction typically ranges from 0.3 - 0.4%.

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