Human IgE ELISA Kit

For detection and measurement of human immunoglobulin E

Human IgE ELISA Kit

For detection and measurement of human immunoglobulin E

From: 856 USD
Catalog #
02032_C
For detection and measurement of human immunoglobulin E

What's Included

  • Human IgE ELISA Kit, 2 plates (Catalog #02032)
    • Human IgE ELISA Plate, 2x
    • Human IgE Standard, 1 vial
    •  Human IgE Biotinylated Detection Antibody, 30 µL
    • SA-HRP, 30 µL
    • Standard Reconstitution Buffer B, 1 mL
    • Wash Buffer (20X), 120 mL
    • ELISA Diluent, 120 mL
    • SA-HRP Diluent, 25 mL
    • TMB Substrate, 25 mL
    •  Stop Solution, 25 mL
    • Adhesive Plate Covers, 6x
 
  • Human IgE ELISA Kit, 10 plates (Catalog #02033)
    • Human IgE ELISA Plate, 10x
    • Human IgE Standard, 1 vial
    • Human IgE Biotinylated Detection Antibody, 130 µL
    • SA-HRP, 130 µL
    • Standard Reconstitution Buffer B, 1 mL
    • Wash Buffer (20X), 5 x 120 mL
    • ELISA Diluent, 3 x 120 mL
    • SA-HRP Diluent, 120 mL
    • TMB Substrate, 120 mL
    • Stop Solution, 120 mL
    • Adhesive Plate Covers, 30x

Overview

The Human Immunoglobulin E (IgE) ELISA Kit is designed for the quantitative detection and measurement of human IgE in biological fluids such as serum, plasma, and cell culture supernatants. IgE is predominantly found in the lungs, skin, and mucous membranes and is the least common immunoglobulin in serum. IgE plays a fundamental role in allergic reactions by stimulating degranulation of eosinophils, mast cells, and basophils through interaction with the Fc epsilon Receptor I (FcεRI).

The assay is based on the sandwich ELISA method, in which samples are added to ELISA strip plates pre-coated with capture antibodies specific for the immunoglobulin. The captured immunoglobulin is detected by addition of a biotinylated detection antibody, followed by streptavidin-horseradish peroxidase (SA-HRP), which binds the biotinylated antibody. Addition of the chromogenic enzyme substrate 3,3’,5,5’ tetramethylbenzidine (TMB) results in a colored product with an intensity directly proportional to the concentration of immunoglobulin in the sample. The concentration of the immunoglobulin is determined by comparison to a serial dilution of the immunoglobulin standard analyzed in parallel.
Subtype
Complete Kits
Cell Type
B Cells, Hybridomas
Species
Human
Area of Interest
Hybridoma Generation, Immunology

Data Figures

Representative Standard Curve

• Reportable Range: 0.1 - 32 ng/mL. This is the concentration range in which measurement of the analyte can be done with the highest precision, accuracy, and linearity.
• Sensitivity: The limit of detection of this assay is 0.069 ng/mL. This is the analyte concentration with absorbance two standard deviations higher than the zero standard.
• Accuracy: The analyte standard of this ELISA was calibrated against NIBSC international standard 75/502.
• Recovery: A mid-curve recovery of 80 - 86% was determined by spiking defined amounts of analyte standard into serum or plasma samples in repeated experiments.
• Precision: The intra-assay precision of this assay is 2.8% (CV). The inter-assay precision of this assay is 5.5% (CV).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
02032, 02033
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
02032, 02033
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
02032, 02033
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Catalog #
02032, 02033
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Catalog #
02032, 02033
Lot #
All
Language
English
Document Type
Safety Data Sheet 5
Catalog #
02032, 02033
Lot #
All
Language
English
Document Type
Safety Data Sheet 6
Catalog #
02032, 02033
Lot #
All
Language
English
Document Type
Safety Data Sheet 7
Catalog #
02032, 02033
Lot #
All
Language
English
Document Type
Safety Data Sheet 8
Catalog #
02032, 02033
Lot #
All
Language
English
Document Type
Safety Data Sheet 9
Catalog #
02032, 02033
Lot #
All
Language
English
Catalog #
02032, 02033
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
02033
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Resources and Publications

Educational Materials (2)

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