BrainPhys™ Imaging Optimized Medium
Serum-free neurophysiological basal medium for improved neuronal live imaging and function
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BrainPhys™ Imaging Optimized Medium -
Label for BrainPhys™ Imaging Optimized Medium
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Required Products
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NeuroCult™ SM1 Neuronal Supplement
Supplement (50X) for the serum-free culture of neurons
Overview
BrainPhys™ Imaging Optimized Medium is a serum-free neuronal basal medium (based on the BrainPhys™ formulation published by Cedric Bardy and Fred H. Gage) that is optimized for imaging applications.
Applications of BrainPhys™ Imaging Optimized Medium include live fluorescent imaging (calcium imaging and optogenetics) and neuronal culture. In addition to the removal of phenol red, the formulation has been modified to reduce background fluorescence and increase stability upon repeated exposure to light (M Zabolocki et al. Nat Comm, 2020).
Applications of BrainPhys™ Imaging Optimized Medium include live fluorescent imaging (calcium imaging and optogenetics) and neuronal culture. In addition to the removal of phenol red, the formulation has been modified to reduce background fluorescence and increase stability upon repeated exposure to light (M Zabolocki et al. Nat Comm, 2020).
Advantages:
• No photoxicity for extended live imaging
• Reduced background autofluorescence in the 488 nm excitation channel
• Perform functional assays without changing media and shocking cells
• More representative of the brain's extracellular environment
• Improved neuronal function and a higher proportion of synaptically active neurons
• Rigorous raw material screening and quality control ensure minimal lot-to-lot variability
• Reduced background autofluorescence in the 488 nm excitation channel
• Perform functional assays without changing media and shocking cells
• More representative of the brain's extracellular environment
• Improved neuronal function and a higher proportion of synaptically active neurons
• Rigorous raw material screening and quality control ensure minimal lot-to-lot variability
Components:
- BrainPhys™ Imaging Optimized Medium (Catalog #05796)
- BrainPhys™ Imaging Optimized, 500 mL
Subtype:
Specialized Media
Cell Type:
Neural Cells, PSC-Derived; Neurons; Pluripotent Stem Cells
Species:
Human; Mouse; Rat; Non-Human Primate; Other
Application:
Cell Culture; Functional Assay; Immunofluorescence; Organoid Culture
Brand:
BrainPhys
Area of Interest:
Disease Modeling; Drug Discovery and Toxicity Testing; Neuroscience; Organoids
Formulation:
Serum-Free; Chemically Defined
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Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
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Data
Figure 1. Schematic for Live Imaging During Differentiation of hPSC-Derived Neurons
Neurons can be transitioned into BrainPhys™ IO with the relevant supplements and cultured for a maximum of 14 days.
Figure 2. Schematic for Live Imaging During Maturation of Primary Tissue-Derived Neurons
Neurons can be transitioned into BrainPhys™ IO with serum replacement supplement and cultured for a maximum of 14 days.
Figure 3. BrainPhys™ IO Reduces Phototoxicity and Autofluorescence of Imaged Cells
Primary rat cortical neurons cultured in BrainPhys™ Imaging Optimized Medium (A) retain a healthy morphology after exposure to blue LED light for 12 hours. (B) Neurons labeled with live neuron dye NeuroFluor™ NeuO showed reduced background fluorescence at a mean emission of 525 nm, resulting in improved image contrast. This medium has enhanced performance under live imaging conditions compared to the original BrainPhys™ Neuronal Medium formulation, which provides superior long-term culture health, but shows (C) some disintegrated cell bodies and neurites (black arrows) and (D) autofluorescence under the same experimental conditions.
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.