Mouse Recombinant PDGF-BB

Platelet-derived growth factor BB

Mouse Recombinant PDGF-BB

Platelet-derived growth factor BB

From: 222 USD
Catalog #
(Select a product)
Platelet-derived growth factor BB
Add to Wish List

Overview

The platelet-derived growth factor (PDGF) family has five heparin-binding members that assemble into four homodimers (PDGF-AA, PDGF-BB, PDGF-CC, and PDGF-DD) and one heterodimer (PDGF-AB; Fretto et al.; Li & Eriksson). PDGF signals through the receptor tyrosine kinases PDGFRα and PDGFRβ. It has been shown that PDGF-induced migration involves signaling pathways involving MEK/ERK, EGFR, Src, and PI3K/AKT (Kim et al.). PDGF is a potent mitogen for cells of mesenchymal origin, such as fibroblasts, glial cells, and vascular smooth muscle cells. PDGF has been implicated in pathogenesis of atherosclerosis, glomerulonephritis, cancer, and in the contraction of vascular smooth muscle cells of rat aortic tissues (Fretto et al.; Sachinidis et al.). PDGF-BB is secreted by osteoblasts to induce mesenchymal stem cell migration and angiogenesis. It has also been shown that PDGF-BB is secreted by preosteoclasts during bone modeling and remodeling to induce angiogenesis and thus proper osteogenesis (Xie et al.).
Subtype
Cytokines, Growth Factors
Cell Type
Mesenchymal Stem and Progenitor Cells, Neural Cells, PSC-Derived, Neural Stem and Progenitor Cells, Neurons, Osteoblasts, Other
Species
Mouse
Area of Interest
Neuroscience, Stem Cell Biology
Purity
≥ 95%

Data Figures

(A) The biological activity of Mouse Recombinant PDGF-BB was tested by its ability to promote the proliferation of BALB/c 3T3 cells. Cell proliferation was measured after 40 hours of culture using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the example above is 0.25 ng/mL. (B) 1 μg of Mouse Recombinant PDGF-BB was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Mouse Recombinant PDGF-BB has a predicted molecular mass of 24.6 kDa (12.4 kDa per monomer).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
78178, 78178.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
78178, 78178.1
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.