RosetteSep™ Human Cord Blood Progenitor Enrichment Kit

Immunodensity negative selection cocktail
Complete RosetteSep™ Human Cord Blood Progenitor Enrichment Kit

Includes RosetteSep™ human cord blood progenitor kit and HetaSep™

500 mL cord blood
Catalog # 15276
467 USD
New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more
Required Products
  1. Lymphoprep™|07851
    Lymphoprep™

    Density gradient medium for the isolation of mononuclear cells

Overview

The RosetteSep™ Human Cord Blood Progenitor Cell Enrichment Cocktail with HetaSep™ is designed to isolate progenitor cells from cord blood by negative selection. The HetaSep™ minimizes the required volume of the RosetteSep™ cocktail reagent by first concentrating the sample by sedimentation with HetaSep™ (Catalog #07906), a solution of hetastarch. The hematopoietic progenitors in the resultant buffy coat are then enriched using a modified RosetteSep™ protocol that uses less RosetteSep™ cocktail than is required for an unconcentrated sample. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing CD2, CD3, CD14, CD16, CD19, CD24, CD56, CD61, CD66b and glycophorin A on red blood cells (RBCs). When centrifuged over a density gradient medium the unwanted cells pellet along with the RBCs. The purified progenitor cells are present as a highly enriched population at the interface between the plasma and the density gradient medium.

We regret to inform our customers that we can no longer include Ficoll-Paque™ Plus with this product. For performing cell isolation by density gradient centrifugation, we recommend purchasing Lymphoprep™ (Catalog #07801), which is available in a variety of sizes and formats. As with Ficoll-Paque™, Lymphoprep™ has a density of 1.077 g/mL, and after centrifugation, mononuclear cells remain at the plasma-Lymphoprep™ interface while granulocytes and erythrocytes sediment below.
Advantages
• Fast and easy-to-use
• Requires no special equipment or training
• Untouched, viable cells
Components
  • RosetteSep™ Human Cord Blood Progenitor Enrichment Kit (Catalog #15276)
    • RosetteSep™ Human Hematopoietic Progenitor Cell Enrichment Cocktail, 2 mL (Catalog #15026) x 2
    • HetaSep™, 100 mL (Catalog #07906)
Subtype
Cell Isolation Kits
Cell Type
Hematopoietic Stem and Progenitor Cells
Species
Human
Sample Source
Cord Blood
Selection Method
Negative
Application
Cell Isolation
Brand
RosetteSep
Area of Interest
Immunology, Stem Cell Biology

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Complete RosetteSep™ Human Cord Blood Progenitor Enrichment Kit
Catalog #
15276
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
Complete RosetteSep™ Human Cord Blood Progenitor Enrichment Kit
Catalog #
15276
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
Complete RosetteSep™ Human Cord Blood Progenitor Enrichment Kit
Catalog #
15276
Lot #
All
Language
English

Educational Materials (7)

Brochure
Cell Separation Products for HLA Analysis
Brochure
HLA Antibodies for Purity Assessment
Wallchart
Frequencies of Cell Types in Human Peripheral Blood
Wallchart
Human Immune Cytokines
Video
Isolate Cells from Whole Blood without Columns or Magnets: RosetteSep™ Immunodensity Cell Separation
2:19
Isolate Cells from Whole Blood without Columns or Magnets: RosetteSep™ Immunodensity Cell Separation
Video
How to Isolate PBMCs from Whole Blood Using the SepMate™ PBMC Isolation Tubes: 15-Minute Protocol
1:46
How to Isolate PBMCs from Whole Blood Using the SepMate™ PBMC Isolation Tubes: 15-Minute Protocol
Scientific Poster
Fast and Easy Hematopoietic Progenitor Cell Enrichment With SepMate™

Frequently Asked Question

What is RosetteSep™?

RosetteSep™ is a rapid cell separation procedure for the isolation of purified cells directly from whole blood, without columns or magnets.

How does RosetteSep™ work?

The antibody cocktail crosslinks unwanted cells to red blood cells (RBCs), forming rosettes. The unwanted cells then pellet with the free RBCs when centrifuged over a density centrifugation medium (e.g. Ficoll-Paque™ PLUS, Lymphoprep™).

What factors affect cell recovery?

The temperature of the reagents can affect cell recovery. All reagents should be at room temperature (sample, density centrifugation medium, PBS, centrifuge) before performing the isolations. Layering can also affect recovery so be sure to carefully layer the sample to avoid mixing with the density centrifugation medium as much as possible. Be sure to collect the entire enriched culture without disturbing the RBC pellet. A small amount of density centrifugation medium can be collected without worry.

Which cell samples can RosetteSep™ be used with?

RosetteSep™ can be used with leukapheresis samples, bone marrow or buffy coat, as long as: the concentration of cells does not exceed 5 x 107 per mL (can dilute if necessary); and there are at least 100 RBCs for every nucleated cell (RBCs can be added if necessary).

Can RosetteSep™ be used with previously frozen or cultured cells?

Yes. Cells should be re-suspended at 2 - 5 x 107 cells / mL in PBS + 2% FBS. Fresh whole blood should be added at 250 µL per mL of sample, as a source of red cells.

Can RosetteSep™ be used to enrich progenitors from cord blood?

Yes. Sometimes cord blood contains immature nucleated red cells that have a lower density than mature RBCs. These immature red cells do not pellet over Ficoll™, which can lead to a higher RBC contamination than peripheral blood separations.

Does RosetteSep™ work with mouse cells?

No, but we have developed EasySep™, a magnetic-based cell isolation system which works with mouse and other non-human species.

Which anticoagulant should be used with RosetteSep™?

Peripheral blood should be collected in heparinized Vacutainers. Cord blood should be collected in ACD.

Should the anticoagulant be washed off before using RosetteSep™?

No, the antibody cocktail can be added directly to the sample.
Read More

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

FACS Profile Results With RosetteSep™ Human Cord Blood Progenitor CellEnrichment Kit

Figure 1. FACS Profile Results With RosetteSep™ Human Cord Blood Progenitor Cell Enrichment Kit

Starting with fresh cord blood, the enrichment of CD34+ cells is typically 29 ± 9%.

Contact STEMCELL Technologies

Our Customer Service, Sales, and Product and Scientific Support departments in North America are available between 6 am and 5 pm Pacific Time (9 am and 8 pm Eastern Time). One of our representatives will be happy to help you by telephone or email. Please complete the form to contact us by email. A representative will get back to you shortly.
  •  

StemCell Technologies Inc. and affiliates ("STEMCELL Technologies") does not share your email address with third parties. StemCell Technologies Inc. will use your email address to confirm your identity and send you newsletters, transaction-related emails, promotional and customer service emails in accordance with our privacy policy. You can change your email preferences at any time.