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Human Peripheral Blood Leukopak, Frozen

Primary human cells, frozen

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New data and packaging, same high quality! Flow cytometry plots are included on the Certificate of Analysis (CoA) for all frozen leukopaks. See Figure 1 in the data section below to view representative plots. Your leukopak may come with external packaging to ensure you get the highest quality product possible upon delivery. Remove all external packaging for optimal thawing performance. Request the CoA for your frozen leukopaks to see the data, and browse our Frequently Asked Questions (FAQs) on Primary Cells for more information.

Human Peripheral Blood Leukopak, Frozen

Primary human cells, frozen

From: 1,707 USD

Select a product option above to browse current inventory and donor details or specify your requirements.

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Primary human cells, frozen
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Product Advantages

  • Isolate large quantities of mononuclear cells from a single donor.
  • Choose from a range of sizes, depending on your research needs.
  • Start your experiments on your schedule with ready-to-use cryopreserved cells.

Overview

Start experiments when you want to with large numbers of leukocytes, including peripheral blood mononuclear cells (PBMCs). As a cryopreserved product, frozen leukopaks provide samples from specific donors that can be processed when the end-user is ready. Leukopaks are available in Full (≥ 9 billion cells), Half (≥ 5 billion cells), Quarter (≥ 2.5 billion cells), and Tenth (≥ 900 million cells) size formats. We help you get the cells you need with personalized service, custom products, flexible delivery times, and multiple size options.

Each frozen leukopak is a highly concentrated, low-volume apheresis collection from a single, normal donor, using Institutional Review Board (IRB)-approved consent forms and protocols. Each full-sized leukopak is produced from ~2 - 3x blood volumes using the Spectra Optia® Apheresis System, with acid-citrate-dextrose solution A (ACDA) as the anticoagulant. The collected product is then cryopreserved in a controlled-rate freezer. High-resolution HLA typing (Class I and Class II alleles and CMV status) are available upon request. Donor specifications (e.g. BMI category, smoking status, ethnicity, etc.) can be requested in the comment box above, after selecting from the product options. Donors are screened for HIV-1, HIV-2, hepatitis B, and hepatitis C.

Certain products are only available in select territories. Please contact your sales representative or Product & Scientific Support at techsupport@stemcell.com for further information.

Browse Frequently Asked Questions (FAQs) on Primary Cells.
Subtype
Frozen
Cell Type
Leukopaks, Mononuclear Cells
Species
Human
Cell and Tissue Source
Peripheral Blood
Area of Interest
Drug Discovery and Toxicity Testing
Donor Status
Normal

Data Figures

Table 1. Frozen Leukopak Size Formats and Minimum Cell Counts

Table listing minimum cell counts for different frozen leukopaks sizes

Frozen leukopaks are available in multiple packaging formats to support different research scales. Each format provides a guaranteed minimum total nucleated cell count. Note: Cell counts represent minimum total nucleated cells per leukopak. Actual cell composition and yields of specific cell populations may vary depending on donor-to-donor variability. Flow cytometry plots are included on the Certificate of Analysis (CoA) for all frozen leukopaks.

Figure 1. Typical Flow Cytometric Analysis Profile of a Leukopak Before Cryopreservation

Representative gating strategy for flow cytometric immunophenotyping of the different cell subpopulations present in a leukopak product prior to cryopreservation. The CD45 plot was gated on viable single cells while all other plots were gated on viable CD45+ single cells. In the above example, the cell frequencies (as a percentage of the overall leukopak product) are as follows: monocytes (CD14+) 19.9%, B cells (CD19+) 13.9%, NK cells (CD3-CD56+) 6.3%, Helper T cells (CD3+CD4+) 28.5%, and Cytotoxic T cells (CD3+CD8+) 13.5%.

Figure 2. Mean Percentages of Cell Subpopulations in a Leukopak

Representative chart showing the average frequencies of major immune subsets in leukopak products, as measured by flow cytometry prior to cryopreservation. Values shown are mean percentages of total viable leukocytes present in a leukopak (n ≥ 30).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Human Peripheral Blood Leukopak, Frozen
Catalog #
200-0132, 200-0131, 200-0130, 200-0470
Lot #
All
Language
English

Applications

Resources and Publications

Educational Materials (10)

Human Peripheral Blood Leukopaks Flyer
Brochure
Human Peripheral Blood Leukopaks Flyer
How to Recover Cells from a Leukocyte Reduction System (LRS) Cone
4:55
Video
How to Recover Cells from a Leukocyte Reduction System (LRS) Cone
How to Process a Leukopak for Downstream Cell Isolation
13:43
Video
How to Process a Leukopak for Downstream Cell Isolation
How to Consistently Thaw Cryobags with the ThawSTAR® CB Automated Thawing System
2:31
Video
How to Consistently Thaw Cryobags with the ThawSTAR® CB Automated Thawing System
How to Create CRISPR-Edited T Cells
36:25
Webinar
How to Create CRISPR-Edited T Cells
Implementing the Colony-Forming Unit (CFU) Assay As a Potency Assay for Hematopoietic Cell Therapy Products
58:50
Webinar
Implementing the Colony-Forming Unit (CFU) Assay As a Potency Assay for Hematopoietic Cell Therapy P...
New Guidelines for T Cell Nomenclature
1:01:42
Webinar
New Guidelines for T Cell Nomenclature
Leukopak Processing: Tips & Tricks for Streamlined Cell Isolation
1:01:09
Webinar
Leukopak Processing: Tips & Tricks for Streamlined Cell Isolation
Application of HSPCs in Cell Therapy Course
On-Demand Training
Application of HSPCs in Cell Therapy Course
Human Immune Cell Isolation Course
On-Demand Training
Human Immune Cell Isolation Course

Frequently Asked Questions

What is the percentage of viable cells in a frozen leukopak?

Upon cryopreservation, cell viability in the frozen leukopak is greater than 90%. After thawing and the cleanup process, the viability is usually greater than 90%.

How does a frozen leukopak differ from a fresh leukopak?

Fresh leukopaks are shipped immediately after collection for same-day processing, while frozen leukopaks are cryopreserved and stored in vapor-phase liquid nitrogen until use. Frozen leukopaks allow for flexible experimental timing, batch testing across multiple donors, and long-term inventory planning. Fresh leukopaks may be preferred when immediate processing and maximal functional activity are required. Learn more about leukopaks here.

Are there any advantages of working with frozen leukopaks compared to fresh leukopaks?

Both fresh and frozen leukopaks are ideal starting materials for downstream cell isolation when large numbers of cells are required, reducing the time and reagents needed to process cells of interest. However, frozen leukopaks provide additional convenience by allowing you to start experiments according to your schedule. Additionally, cell viability may be better preserved in frozen leukopaks during global shipping. Frozen leukopaks may also provide you with access to samples from the same lot at a future date.

How should frozen leukopaks be thawed and handled?

Frozen leukopaks should be rapidly thawed using validated protocols to maximize post-thaw recovery and viability. The ThawSTAR® CB Automated Thawing System (Catalog #100-1151) provides an automated thawing procedure and eliminates risk of biosample loss and contamination with single-use barrier bags. Watch this step-by-step protocol video for more information.
Following thaw, PBMCs can be isolated or immune subsets enriched using immunomagnetic separation methods. Proper thawing and handling are critical to preserve cell functionality for downstream immune assays and expansion workflows.

What are frozen leukopaks used for?

Frozen leukopaks are commonly used in: CAR-T and CAR-NK research; T cell expansion and activation; NK cell cytotoxicity and potency assays; ADCC assays; gene editing and transduction workflows; and assay standardization across donor cohorts. They are particularly useful when consistent donor material is required over time.

What donor screening and documentation are available for frozen leukopaks?

Frozen leukopaks are sourced from consented healthy donors under IRB-approved protocols. Donor eligibility criteria, demographic information, and infectious disease screening documentation are available to support research traceability and compliance.

Related Products

Related Products
New data and packaging, same high quality! Flow cytometry plots are included on the Certificate of Analysis (CoA) for all frozen leukopaks. See Figure 1 in the data section below to view representative plots. Your leukopak may come with external packaging to ensure you get the highest quality product possible upon delivery. Remove all external packaging for optimal thawing performance. Request the CoA for your frozen leukopaks to see the data, and browse our Frequently Asked Questions (FAQs) on Primary Cells for more information.
Legal Statement:

Except as permitted by law, any unauthorized use, duplication and/or distribution of this content without express and written permission from STEMCELL is strictly prohibited. Use of donor information, including but not limited to HLA and other genetic information, for the purpose of identifying the donor or any genealogical analysis is strictly prohibited. All donor information must be used within the scope of the donor’s consent and in accordance with all applicable laws, rules and regulations, including but not limited to all applicable requirements of (a) Title 28 of the Code of Federal Regulations, Part 202 (28 CFR 202) - Access to U.S. Sensitive Personal Data and Government-Related Data by Countries of Concern or Covered Persons; and (b) the Protecting Americans' Data From Foreign Adversaries Act of 2024 (15 USC 9901).

Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.

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