Human IgG ELISA Antibody Pair Kit

For detection and measurement of human immunoglobulin G

New! For your convenience, all of our antibody pair kits now include a vial of Standard Reconstitution Buffer B. This change does not affect product performance or quality.

Human IgG ELISA Antibody Pair Kit

For detection and measurement of human immunoglobulin G

From: 521 USD
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For detection and measurement of human immunoglobulin G
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What's Included

  • Human IgG Capture Antibody, 300 µL
  • Human IgG ALP-Conjugated Detection Antibody, 80 µL
  • Human IgG Standard, 1 vial
  • Standard Reconstitution Buffer B, 1 mL
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.

Overview

The Human Immunoglobulin G (IgG) ELISA Antibody Pair Kit is intended for those who want the flexibility of setting up their own ELISA assay. This kit includes capture and detection antibodies and an IgG standard. It is designed for the quantitative detection and measurement of total human IgG in biological fluids such as serum, plasma, and cell culture supernatants. IgG is the major immunoglobulin found in blood, lymph fluid, cerebrospinal fluid, and peritoneal fluid. IgG plays a key role in the humoral immune response, plays a critical step in the opsonization process by interacting with the Fcγ receptor on macrophages, neutrophils, and natural killer cells, and is able to activate the complement system. IgG is the most abundant immunoglobulin in serum, comprising approximately 80% of all serum immunoglobulins.

The assay is based on the sandwich ELISA method, in which samples are added to ELISA plates coated with capture antibodies specific for the immunoglobulin. The captured immunoglobulin is detected by addition of a detection antibody conjugated to alkaline phosphatase (ALP). Addition of the chromogenic enzyme substrate p-nitrophenyl phosphate (pNPP) results in a colored product with an intensity directly proportional to the concentration of immunoglobulin in the sample. The concentration of the immunoglobulin is determined by comparison to a serial dilution of the immunoglobulin standard analyzed in parallel.

NOTE: This kit includes sufficient reagents for 6 x 96-well ELISA plates (Catalog #38019). ELISA plates and pNPP Substrate (Catalog #01917) are required for use with the Human IgG ELISA Antibody Pair Kit and are available for purchase separately.
Subtype
Antibody Pair Kits
Cell Type
B Cells, Hybridomas
Species
Human
Area of Interest
Hybridoma Generation, Immunology

Data Figures

Representative Standard Curve

• Reportable Range: 0.32 - 316 ng/mL. This is the concentration range in which measurement of the analyte can be done with the highest precision, accuracy, and linearity.
• Accuracy: The analyte standard of this ELISA was calibrated against NIBSC international standard 67/086.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

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Language
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01994
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All
Language
English
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Safety Data Sheet 1
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01994
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All
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English
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Safety Data Sheet 2
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01994
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All
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English
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Safety Data Sheet 3
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01994
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All
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English
Document Type
Safety Data Sheet 4
Catalog #
01994
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Resources and Publications

Educational Materials (2)

New! For your convenience, all of our antibody pair kits now include a vial of Standard Reconstitution Buffer B. This change does not affect product performance or quality.