EasySep™ Release Mouse CD138 Positive Selection Kit

Immunomagnetic positive selection kit using particle release technology

EasySep™ Release Mouse CD138 Positive Selection Kit

Immunomagnetic positive selection kit using particle release technology

EasySep™ Release Mouse CD138 Positive Selection Kit
2 x 10^9 cells
937 USD
Catalog # 100-0601

Immunomagnetic positive selection of particle-free mouse CD138+ cells

Product Advantages


  • Up to 95% purity of plasma cells from immunized mouse samples

  • No-wash removal of EasySep™ Releasable RapidSpheres™

  • Compatible with hybridoma generation protocols, including electrofusion

What's Included

  • EasySep™ Release Mouse CD138 Positive Selection Cocktail, 1 x 1 mL
  • EasySep™ Mouse FcR Blocker, 2 x 0.5 mL
  • EasySep™ Isolation Cocktail Enhancer, 1 x 1 mL
  • EasySep™ Releasable RapidSpheres™ 50201, 1 x 1 mL
  • EasySep™ Release Buffer (Concentrate), 3 x 1 mL

Overview

Isolate magnetic particle-free CD138+ plasma cells and plasmablasts from single-cell suspensions of mouse splenocytes or bone marrow in as little as 37 minutes. CD138+ cells are differentiated B cells that can be used in antibody discovery research due to their ability to generate affinity-matured antibodies. While hybridoma fusions with mouse bone marrow and splenocytes typically require screening thousands of colonies, enriching for CD138+ plasma cells prior to hybridoma fusion can substantially decrease the screening burden to generate hybridomas.

To achieve higher lab efficiency for your antibody discovery research, purify CD138+ plasma cells and plasmablasts without columns by using immunomagnetic positive selection. Simply label cells with antibodies and magnetic particles, place in an EasySep™ cell separation magnet, then remove the bound magnetic particles from the isolated CD138+ cell fraction. CD138+ cells isolated from immunized mice are up to 95% pure and can be used with hybridoma generation protocols such as electrofusion.

Visit www.stemcell.com/easysep-release to learn more.
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
Subtype
Cell Isolation Kits
Cell Type
B Cells
Species
Mouse
Sample Source
Bone Marrow, Lymph Node, Other, Spleen
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep
Area of Interest
Hybridoma Generation, Immunology, Infectious Diseases

Data Figures

Using the EasySep™ Release Mouse CD138 Positive Selection Kit on a single-cell suspension of mouse spleen, the CD138+ purities of the start and final isolated fractions are 0.6% and 77.6% in naïve, and 1.2% and 91.6% in immunized mice, respectively.

Figure 1. EasySep™ Release Mouse CD138 Positive Selection Kit

(A) Starting with naïve C57BL/6 mouse splenocytes, the plasma cell (CD138+CD267 (TACI)+) content of the isolated fraction is typically 77.2 ± 2.4% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and isolated fractions are 0.6% and 77.6%, respectively. (B) Starting with immunized BALB/c mouse splenocytes, the plasma cell (CD138+CD267 (TACI)+) content of the isolated fraction is typically 86.1 ± 7.4% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and isolated fractions are 1.2% and 91.6%, respectively.

Using the EasySep™ Release Mouse CD138 Positive Selection Kit on a single-cell suspension of mouse bone marrow, the CD138+ purities of the start and final isolated fractions are 0.3% and 71.7% in naïve, and 0.6% and 89.0% in immunized mice, respectively.

C) Starting with naïve C57BL/6 mouse bone marrow, the plasma cell (CD138+CD267 (TACI)+) content of the isolated fraction is typically 62.0 ± 13.1% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and isolated fractions are 0.3% and 71.1%, respectively. (D) Starting with immunized BALB/c mouse bone marrow, the plasma cell (CD138+CD267 (TACI)+) content of the isolated fraction is typically 85.5 ± 9.8% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and isolated fractions are 0.6% and 89.0%, respectively.

In immunized mice, the % antigen-specific hit rate for total splenocytes or EasySep™-isolated CD138+ cells from splenocytes were 3.2% and 56.1%, respectviely. The % antigen-specific hit rate for total bone marrow or EasySep™-isolated CD138+ cells from bone marrow were 0% and 31.9%, respectviely.

(E) Isolated CD138+ cells or total splenocytes from mice immunized with various antigens were fused with Sp2/0 mouse myeloma cells and plated in semi-solid medium using ClonaCell™-HY Hybridoma Kit (Catalog #03800). The % antigen-specific hit rate was determined by ELISA. The % antigen-specific hit rates for total splenocytes and CD138+ cells were 3.2 ± 4.0% and 56.1 ± 37.2% (mean ± SD), respectively. (F) Isolated CD138+ cells or total bone marrow cells from mice immunized with various antigens were fused with Sp2/0 mouse myeloma cells and plated in semi-solid medium using ClonaCell™-HY Hybridoma Kit (Catalog #03800). The % antigen-specific hit rate was determined by ELISA. The % antigen-specific hit rates for total bone marrow cells and CD138+ cells were 0% and 31.9 ± 46.0% (mean ± SD), respectively.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
100-0601
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
100-0601
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
100-0601
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Catalog #
100-0601
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Catalog #
100-0601
Lot #
All
Language
English
Document Type
Safety Data Sheet 5
Catalog #
100-0601
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Contact STEMCELL Technologies

Our Customer Service, Sales, and Product and Scientific Support departments in North America are available between 6 am and 5 pm Pacific Time (9 am and 8 pm Eastern Time). One of our representatives will be happy to help you by telephone or email. Please complete the form to contact us by email. A representative will get back to you shortly.
  •  

StemCell Technologies Inc. and affiliates ("STEMCELL Technologies") does not share your email address with third parties. StemCell Technologies Inc. will use your email address to confirm your identity and send you newsletters, transaction-related emails, promotional and customer service emails in accordance with our privacy policy. You can change your email preferences at any time.