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EasySep™ Human Cord Blood CD34 Positive Selection Kit III

Immunomagnetic positive selection of human CD34+ cells from fresh whole umbilical cord blood using a simple, two-step procedure

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New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more

EasySep™ Human Cord Blood CD34 Positive Selection Kit III

Immunomagnetic positive selection of human CD34+ cells from fresh whole umbilical cord blood using a simple, two-step procedure

From: 839 USD
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Immunomagnetic positive selection of human CD34+ cells from fresh whole umbilical cord blood using a simple, two-step procedure
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Product Advantages


  • Fast and easy-to-use

  • Up to 96% purity

  • No columns required

  • Can be combined with SepMate™ for consistent, high-throughput sample processing

What's Included

  • EasySep™ Human Cord Blood CD34 Positive Selection Kit III (Catalog #17897)
    • RosetteSep™ Human Progenitor Cell Basic Pre-Enrichment Cocktail, 2 x 2.5 mL
    • EasySep™ Human CD34 Positive Selection Cocktail, 2 x 1 mL
    • EasySep™ Dextran RapidSpheres™ 50100, 1 mL
  • RoboSep™ Human Cord Blood CD34 Positive Selection Kit III (Catalog #17897RF)
    • RosetteSep™ Human Progenitor Cell Basic Pre-Enrichment Cocktail, 2 x 2.5 mL
    • EasySep™ Human CD34 Positive Selection Cocktail, 2 x 1 mL
    • EasySep™ Dextran RapidSpheres™ 50100, 1 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. EasySep™ Magnet
    EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer for use with EasySep™ cell separation kits

Overview

Easily isolate highly purified human CD34+ cells from fresh whole umbilical cord blood (CB) samples, using immunomagnetic positive selection, with the EasySep™ Human Cord Blood CD34 Positive Selection Kit III. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

First, hematopoietic progenitor cells are pre-enriched using the RosetteSep™ Human Progenitor Cell Basic Pre-Enrichment Cocktail (15226C) with antibodies recognizing T cell, B cell, and myeloid cell surface markers. CD34+ cells are then selected using the EasySep™ Human CD34 Positive Selection Cocktail (18096C). Desired cells are labeled with antibody complexes recognizing CD34 and magnetic particles. Labeled cells are separated using an EasySep™ magnet and by simply pouring or pipetting off the unwanted cells. The cells of interest remain in the tube. Following magnetic cell isolation, the desired CD34+ cells are ready for downstream applications.

This kit does not deplete platelets. If isolating CD34+ cells from whole CB in which platelet contamination is expected, the EasySep™ Human Cord Blood CD34 Positive Selection Kit II (Catalog #17896) is recommended.


If isolating CD34+ cells from fresh blood or buffy coat, the Complete Kit for Human Whole Blood CD34+ Cells (Catalog #15086) is recommended.

If isolating CD34+ cells from other samples, including fresh or previously frozen mobilized peripheral blood or bone marrow mononuclear cells, or from previously frozen cord blood mononuclear cells, we recommend using the EasySep™ Human CD34 Positive Selection Kit II (Catalog #17856).

Learn more about how immunomagnetic EasySep™ technology works or how to fully automate immunomagnetic cell isolation with RoboSep™. Explore additional products optimized for your workflow, including culture media, supplements, antibodies, and more.

Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™ (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
Hematopoietic Stem and Progenitor Cells
Species
Human
Sample Source
Cord Blood
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep
Area of Interest
Immunology, Stem Cell Biology

Data Figures

Figure 1. Typical EasySep™ Human Cord Blood CD34 Positive Selection III Isolation Profile

Starting with fresh cord blood, the CD34+ cell content of the isolated fraction is typically 87 ± 12% (mean ± SD, n=10, data obtained using the purple EasySep™ Magnet). The CD34+ cell content of the starting sample is typically 0.5 ± 0.25% (mean ± SD).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
RoboSep™ Human Cord Blood CD34 Positive Selection Kit III
Catalog #
17897RF
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
EasySep™ Human Cord Blood CD34 Positive Selection Kit III
Catalog #
17897
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ Human Cord Blood CD34 Positive Selection Kit III
Catalog #
17897RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ Human Cord Blood CD34 Positive Selection Kit III
Catalog #
17897RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ Human Cord Blood CD34 Positive Selection Kit III
Catalog #
17897RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
RoboSep™ Human Cord Blood CD34 Positive Selection Kit III
Catalog #
17897RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Human Cord Blood CD34 Positive Selection Kit III
Catalog #
17897
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Human Cord Blood CD34 Positive Selection Kit III
Catalog #
17897
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
EasySep™ Human Cord Blood CD34 Positive Selection Kit III
Catalog #
17897
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (9)

EasySep™ Cell Separation Technology
Brochure
EasySep™ Cell Separation Technology
Hematopoietic Stem and Progenitor Cells - Products for Your Research
Brochure
Hematopoietic Stem and Progenitor Cells - Products for Your Research
Human CD34+ Cell Isolation Product Selection
Brochure
Human CD34+ Cell Isolation Product Selection
Isolation of CD34+ Cells from Human Cord Blood
Technical Bulletin
Isolation of CD34+ Cells from Human Cord Blood
Human Immune Cytokines
Wallchart
Human Immune Cytokines
Simultaneous Cell Isolation from Multiple Samples Using the EasyEights™ EasySep™ Magnet
0:57
Video
Simultaneous Cell Isolation from Multiple Samples Using the EasyEights™ EasySep™ Magnet
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep™)
1:37
Video
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep...
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
Video
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
1:13
Video
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology

Frequently Asked Questions

Can EasySep™ be used for either positive or negative selection?

Yes. The EasySep™ kits use either a negative selection approach by targeting and removing unwanted cells or a positive selection approach targeting desired cells. Depletion kits are also available for the removal of cells with a specific undesired marker (e.g. GlyA).

How does the separation work?

Magnetic particles are crosslinked to cells using Tetrameric Antibody Complexes (TAC). When placed in the EasySep™ Magnet, labeled cells migrate to the wall of the tube. The unlabeled cells are then poured off into a separate fraction.

Which columns do I use?

The EasySep™ procedure is column-free. That's right - no columns!

How can I analyze the purity of my enriched sample?

The Product Information Sheet provided with each EasySep™ kit contains detailed staining information.

Can EasySep™ separations be automated?

Yes. RoboSep™, the fully automated cell separator, automates all EasySep™ labeling and cell separation steps.

Can EasySep™ be used to isolate rare cells?

Yes. We recommend a cell concentration of 2x108 cells/mL and a minimum working volume of 100 µL. Samples containing 2x107 cells or fewer should be suspended in 100 µL of buffer.

Are the EasySep™ magnetic particles FACS-compatible?

Yes, the EasySep™ particles are flow cytometry-compatible, as they are very uniform in size and about 5000X smaller than other commercially available magnetic beads used with column-free systems.

Can the EasySep™ magnetic particles be removed after enrichment?

No, but due to the small size of these particles, they will not interfere with downstream applications.

Can I alter the separation time in the magnet?

Yes; however, this may impact the kit's performance. The provided EasySep™ protocols have already been optimized to balance purity, recovery and time spent on the isolation.

For positive selection, can I perform more than 3 separations to increase purity?

Yes, the purity of targeted cells will increase with additional rounds of separations; however, cell recovery will decrease.

How does the binding of the EasySep™ magnetic particle affect the cells? is the function of positively selected cells altered by the bound particles?

Hundreds of publications have used cells selected with EasySep™ positive selection kits for functional studies. Our in-house experiments also confirm that selected cells are not functionally altered by the EasySep™ magnetic particles.

If particle binding is a key concern, we offer two options for negative selection. The EasySep™ negative selection kits can isolate untouched cells with comparable purities, while RosetteSep™ can isolate untouched cells directly from whole blood without using particles or magnets.

What is RosetteSep™?

RosetteSep™ is a rapid cell separation procedure for the isolation of purified cells directly from whole blood, without columns or magnets.

How does RosetteSep™ work?

The antibody cocktail crosslinks unwanted cells to red blood cells (RBCs), forming rosettes. The unwanted cells then pellet with the free RBCs when centrifuged over a density centrifugation medium (e.g. Ficoll-Paque™ PLUS, Lymphoprep™).

What factors affect cell recovery?

The temperature of the reagents can affect cell recovery. All reagents should be at room temperature (sample, density centrifugation medium, PBS, centrifuge) before performing the isolations. Layering can also affect recovery so be sure to carefully layer the sample to avoid mixing with the density centrifugation medium as much as possible. Be sure to collect the entire enriched culture without disturbing the RBC pellet. A small amount of density centrifugation medium can be collected without worry.

Which cell samples can RosetteSep™ be used with?

RosetteSep™ can be used with leukapheresis samples, bone marrow or buffy coat, as long as: the concentration of cells does not exceed 5 x 107 per mL (can dilute if necessary); and there are at least 100 RBCs for every nucleated cell (RBCs can be added if necessary).

Can RosetteSep™ be used with previously frozen or cultured cells?

Yes. Cells should be re-suspended at 2 - 5 x 107 cells / mL in PBS + 2% FBS. Fresh whole blood should be added at 250 µL per mL of sample, as a source of red cells.

Can RosetteSep™ be used to enrich progenitors from cord blood?

Yes. Sometimes cord blood contains immature nucleated red cells that have a lower density than mature RBCs. These immature red cells do not pellet over Ficoll™, which can lead to a higher RBC contamination than peripheral blood separations.

Does RosetteSep™ work with mouse cells?

No, but we have developed EasySep™, a magnetic-based cell isolation system which works with mouse and other non-human species.

Which anticoagulant should be used with RosetteSep™?

Peripheral blood should be collected in heparinized Vacutainers. Cord blood should be collected in ACD.

Should the anticoagulant be washed off before using RosetteSep™?

No, the antibody cocktail can be added directly to the sample.

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New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more
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