EasySep™ Human Cord Blood CD34 Positive Selection Kit III
Overview
This kit does not deplete platelets. If isolating CD34+ cells from whole CB in which platelet contamination is expected the EasySep™ Human Cord Blood CD34 Positive Selection Kit II (Catalog #17896) is recommended.
If isolating CD34+ cells from fresh blood or buffy coat, the Complete Kit for Human Whole Blood CD34+ Cells (Catalog #15086) is recommended.
If isolating CD34+ cells from other samples, including fresh or previously frozen mobilized peripheral blood or bone marrow mononuclear cells, or from previously frozen cord blood mononuclear cells, we recommend using the EasySep™ Human CD34 Positive Selection Kit II (Catalog #17856).
• Up to 96% purity
• No columns required
• Can be combined with SepMate™ for consistent, high-throughput sample processing
- EasySep™ Human Cord Blood CD34 Positive Selection Kit III (Catalog #17897)
- RosetteSep™ Human Progenitor Cell Basic Pre-Enrichment Cocktail, 2 x 2.5 mL
- EasySep™ Human CD34 Positive Selection Cocktail, 2 x 1 mL
- EasySep™ Dextran RapidSpheres™ 50100, 1 mL
- RoboSep™ Human Cord Blood CD34 Positive Selection Kit III (Catalog #17897RF)
- RosetteSep™ Human Progenitor Cell Basic Pre-Enrichment Cocktail, 2 x 2.5 mL
- EasySep™ Human CD34 Positive Selection Cocktail, 2 x 1 mL
- EasySep™ Dextran RapidSpheres™ 50100, 1 mL
- RoboSep™ Buffer (Catalog #20104)
- RoboSep™ Filter Tips (Catalog #20125)
Scientific Resources
Product Documentation
Document Type | Product Name | Catalog # | Lot # | Language |
---|---|---|---|---|
Document Type
Product Information Sheet
|
Product Name
EasySep™ Human Cord Blood CD34 Positive Selection Kit III
|
Catalog #
17897 |
Lot #
All |
Language
English |
Document Type
Product Information Sheet
|
Product Name
RoboSep™ Human Cord Blood CD34 Positive Selection Kit III
|
Catalog #
17897RF |
Lot #
All |
Language
English |
Document Type
Safety Data Sheet 1
|
Product Name
EasySep™ Human Cord Blood CD34 Positive Selection Kit III
|
Catalog #
17897 |
Lot #
All |
Language
English |
Document Type
Safety Data Sheet 2
|
Product Name
EasySep™ Human Cord Blood CD34 Positive Selection Kit III
|
Catalog #
17897 |
Lot #
All |
Language
English |
Document Type
Safety Data Sheet 3
|
Product Name
EasySep™ Human Cord Blood CD34 Positive Selection Kit III
|
Catalog #
17897 |
Lot #
All |
Language
English |
Document Type
Safety Data Sheet 1
|
Product Name
RoboSep™ Human Cord Blood CD34 Positive Selection Kit III
|
Catalog #
17897RF |
Lot #
All |
Language
English |
Document Type
Safety Data Sheet 2
|
Product Name
RoboSep™ Human Cord Blood CD34 Positive Selection Kit III
|
Catalog #
17897RF |
Lot #
All |
Language
English |
Document Type
Safety Data Sheet 3
|
Product Name
RoboSep™ Human Cord Blood CD34 Positive Selection Kit III
|
Catalog #
17897RF |
Lot #
All |
Language
English |
Educational Materials (9)
Frequently Asked Question
What is RosetteSep™?
How does RosetteSep™ work?
What factors affect cell recovery?
Which cell samples can RosetteSep™ be used with?
Can RosetteSep™ be used with previously frozen or cultured cells?
Can RosetteSep™ be used to enrich progenitors from cord blood?
Does RosetteSep™ work with mouse cells?
Which anticoagulant should be used with RosetteSep™?
Should the anticoagulant be washed off before using RosetteSep™?
Can EasySep™ be used for either positive or negative selection?
How does the separation work?
Which columns do I use?
How can I analyze the purity of my enriched sample?
Can EasySep™ separations be automated?
Can EasySep™ be used to isolate rare cells?
Are the EasySep™ magnetic particles FACS-compatible?
Can the EasySep™ magnetic particles be removed after enrichment?
Can I alter the separation time in the magnet?
For positive selection, can I perform more than 3 separations to increase purity?
How does the binding of the EasySep™ magnetic particle affect the cells? is the function of positively selected cells altered by the bound particles?
If particle binding is a key concern, we offer two options for negative selection. The EasySep™ negative selection kits can isolate untouched cells with comparable purities, while RosetteSep™ can isolate untouched cells directly from whole blood without using particles or magnets.
Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Data and Publications
Data

Figure 1. Typical EasySep™ Human Cord Blood CD34 Positive Selection III Isolation Profile
Starting with fresh cord blood, the CD34+ cell content of the isolated fraction is typically 87 ± 12% (mean ± SD, n=10, data obtained using the purple EasySep™ Magnet). The CD34+ cell content of the starting sample is typically 0.5 ± 0.25% (mean ± SD).
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.