CHO Cell 96-Well Plate Semi-Solid Cloning Procedure

Semi-solid cloning of CHO cells in 96-well plates is an efficient cloning method that enables selective expansion of only those clones producing the protein of interest. This allows you to isolate producing clones with a high probability of monoclonality in a single cloning step. This video outlines the ClonaCell™-CHO 96-well plate semi-solid cloning protocol, including preparation of the cells, plating the semi-solid cultures into 96-well plates, overlaying the cultures with liquid medium, screening to identify producing clones and picking colonies of interest for expansion.
Publish Date: August 29, 2013 Views: 2893