ClonaCell™-CHO AOF Supplement
Animal origin-free, serum-free, L-glutamine-free, and phenol red-free 40X culture supplement for cloning during CHO cell line development
ClonaCell™-CHO AOF Supplement
Animal origin-free, serum-free, L-glutamine-free, and phenol red-free 40X culture supplement for cloning during CHO cell line development
Product Advantages
- Significantly increases single-cell cloning efficiency of CHO cells in protein-free liquid or semi-solid media
- Compatible with a variety of commercially available liquid or semi-solid protein-free media
- Allows the user to maintain existing upstream and downstream cell culture processes
Overview
Formulated without phenol red, this supplement offers precipitate-free culture for improved imaging clarity and visualization of clonality, compared to ClonaCell™-CHO ACF Supplement (Catalog #03820). This animal origin-free (AOF) supplement is formulated with no animal-derived raw materials to the secondary level of manufacturing. It contains recombinant proteins and chemically defined components and is free of serum, hydrolysates, L-glutamine, or selection agents.
Use this 40X supplement with a variety of base media suitable for CHO cell culture, including protein-free ClonaCell™-CHO CD Liquid Medium (Catalog #03817) or semi-solid ClonaCell™-CHO CD Medium (Catalog #03815).
Explore liquid and semi-solid media for efficient cell cloning and cell line generation in the ClonaCell™ brand page.
Learn more about how we define our culture media and supplements, including AOF media.
Data Figures
Figure 1. Cloning Efficiencies for Subcloned CHO-S and CHO-K1 Cells
CHO-S (A) and CHO-K1 (B) cells were subcloned by limiting dilution in DMEM + 10% FBS, or ClonaCell™-CHO CD Liquid Medium containing either ClonaCell™-CHO ACF Supplement or ClonaCell™-CHO AOF Supplement; all conditions contained 6 - 8 mM L-glutamine. Individual wells of 96-well plates were seeded with an average of 0.5 or 1 cell per well in 200 μL culture medium. After incubation for 14 days (37°C, 5% CO2) the plates were examined under a microscope and assessed for growth, with wells containing >100 cells considered positive for outgrowth. The cloning efficiency was estimated by Poisson statistics using the ELDA method described by Hu & Smith (2009). Data are shown as mean ± 1 SD for n = 4 independent experiments.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (7)
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Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
