ClonaCell™-CHO AOF Supplement

Animal origin-free, serum-free, L-glutamine-free, and phenol red-free 40X culture supplement for cloning during CHO cell line development
ClonaCell™-CHO AOF Supplement

Animal origin-free, serum-free, L-glutamine-free, and phenol red-free 40X culture supplement for cloning during CHO cell line development

2.5 mL
Catalog # 100-0382
139 USD

Overview

Save time and your precious clones during cell line development by increasing cloning efficiency and promoting robust growth of Chinese hamster ovary (CHO) cells with ClonaCell™-CHO AOF Supplement in your base medium.

Formulated without phenol red, this supplement offers precipitate-free culture for improved imaging clarity and visualization of clonality, compared to ClonaCell™-CHO ACF Supplement (Catalog #03820). This animal origin-free (AOF) supplement is formulated with no animal-derived raw materials to the secondary level of manufacturing. It contains recombinant proteins and chemically defined components and is free of serum, hydrolysates, L-glutamine, or selection agents.

Use this 40X supplement with a variety of base media suitable for CHO cell culture, including protein-free ClonaCell™-CHO CD Liquid Medium (Catalog #03817) or semi-solid ClonaCell™-CHO CD Medium (Catalog #03815).

Explore liquid and semi-solid media for efficient cell cloning and cell line generation in the ClonaCell™ brand page.

Learn more about how we define our culture media and supplements, including AOF media.
Advantages
• Significantly increases single-cell cloning efficiency of CHO cells in protein-free liquid or semi-solid media
• Compatible with a variety of commercially available liquid or semi-solid protein-free media
• Allows the user to maintain existing upstream and downstream cell culture processes
Subtype
Supplements
Cell Type
CHO Cells
Species
Other
Application
Cell Culture
Brand
ClonaCell
Area of Interest
Antibody Development, Cell Line Development, Drug Discovery and Toxicity Testing
Formulation
Animal Origin-Free, Serum-Free

Related Products

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
ClonaCell™-CHO AOF Supplement
Catalog #
100-0382
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
ClonaCell™-CHO AOF Supplement
Catalog #
100-0382
Lot #
All
Language
English

Educational Materials(8)

Brochure
ClonaCell™-CHO ACF Supplement for Robust Growth of CHO Cells at Low Cell Density
Brochure
ClonaCell-CHO Semi-Solid Cloning Testing Guidelines
Brochure
ClonaCell™ Media for Hybridoma and Cell Line Development
Technical Bulletin
Protocol for Cloning CHO Cell Lines Using ClonaCell™-CHO Semi-Solid Medium
Video
21:01
Video Tutorial: Probability of Monoclonality
Video
12:47
How to Perform Semi-Solid Cloning of CHO Cell Lines in 96-Well Plates Using ClonaCell™-CHO Medium
Video
10:50
Semi-Solid Cloning of CHO Cells Using ClonaCell™-CHO Medium
Webinar
34:50
A Smarter Way To Clone: What You Don't Know About Limiting Dilution Can Hurt Your Probability of Monoclonality

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Comparison of the cloning efficiency (%) of CHO-S and CHO-K1 cells subcloned in DMEM + 10% FBS, or ClonaCell™-CHO CD Liquid Medium containing either ClonaCell™-CHO ACF Supplement or ClonaCell™-CHO AOF Supplement.

Figure 1. Cloning Efficiencies for Subcloned CHO-S and CHO-K1 Cells

CHO-S (A) and CHO-K1 (B) cells were subcloned by limiting dilution in DMEM + 10% FBS, or ClonaCell™-CHO CD Liquid Medium containing either ClonaCell™-CHO ACF Supplement or ClonaCell™-CHO AOF Supplement; all conditions contained 6 - 8 mM L-glutamine. Individual wells of 96-well plates were seeded with an average of 0.5 or 1 cell per well in 200 μL culture medium. After incubation for 14 days (37°C, 5% CO2) the plates were examined under a microscope and assessed for growth, with wells containing >100 cells considered positive for outgrowth. The cloning efficiency was estimated by Poisson statistics using the ELDA method described by Hu & Smith (2009). Data are shown as mean ± 1 SD for n = 4 independent experiments.

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