Centrifugation Speed and Time for Cells
Optimal centrifugation conditions are required for specific cell types. This guide provides centrifugation speed, time & temperature for various protocols
Optimal centrifugation procedures are required for specific cell types and samples. Below is a quick guide for the centrifugation speed, time, and temperature that we recommend for use with different protocols.
Centrifugation Procedures for Washing Cells
Method
Centrifugation Speed
Centrifugation Time
Centrifugation Temperature
Brake Setting (On/Off)
Regular Cell Wash
300 x g
5 - 10 min
Room temperature*
On
Gentle Cell Wash
100 x g
5 - 6 min
Room temperature
On
Thawed Cell Wash
300 x g
5 - 10 min
2 - 8 °C
On
Platelet Removal Wash
120 x g
10 min
Room temperature
Off
*Room temperature: 15 - 25 °C
Centrifugation Procedures by Cell Separation Method
Method
Centrifugation Speed
Centrifugation Time
Centrifugation Temperature
Brake Setting (On/Off)
Ficoll-Paque™ or Lymphoprep™
Density gradient medium for mononuclear cell isolation
Density gradient medium for mononuclear cell isolation
400 x g
(Or: 1200 x g)
(Or: 1200 x g)
30 min
(Or: 20 min)
(Or: 20 min)
Room temperature
Off
330 x g
25 min
Room temperature
Off
1200 x g
Fresh sample: 10 min
> 24 hrs old: 20 min
> 24 hrs old: 20 min
Room temperature
Off
Centrifugation Procedures for Processing Neurospheres
Cell Type
Centrifugation Speed
Centrifugation Time
Centrifugation Temperature
Brake Setting (On/Off)
Neurospheres
90 x g
5 min
Room temperature
On
Dissociated Neurospheres
150 x g
5 min
Room temperature
On
Related Resources
Related Products
Lymphoprep™ Density Gradient Medium
A cost-effective alternative to Ficoll-Paque™ that can be used with SepMate™ for the isolation of mononuclear cells from blood.
RosetteSep™ Immunodensity Cell Separation
Isolate human cells directly from whole blood during density gradient centrifugation. Combine with SepMate™ for a faster protocol.
