STEMCELL's products , , and can be used to isolate hematopoietic stem and progenitor cells (HSPCs) with high purity and recovery. Cells can be used immediately following isolation for downstream functional and biological studies.

Isolation of Human CD34+ Hematopoietic Progenitor Cells:

CELL TYPE	SOURCE	PRODUCT NAME	CATALOG #	PURITY	CAPACITY
CD34 + Cells	Mononuclear cells from mobilized peripheral blood, cord blood or bone marrow and hESC/hiPSC-derived hematopoietic cells	EasySep™ Human CD34 Positive Selection Kit II	17856/17856RF*	92.4 - 94.6%	For labeling up to 5x109 cells
	Whole Blood	Complete Kit for Human Whole Blood CD34+ Cells	15086/15086RF	79.0-95.0%	For labeling 120 mL of whole blood
	Cord Blood	EasySep™ Human Cord Blood CD34 Positive Selection Kit II	17896/17896RF	91.0± 9.0%	For labeling 100 mL of cord blood
Hematopoietic Progenitor Cells	Cord Blood	EasySep™ Human Progenitor Enrichment Kit with Platelet Depletion	19356/19356RF	50.0 – 75.0% CD34+ cells	For labeling up to 1 x 109 cells
	Bone Marrow	RosetteSep™ Human Bone Marrow Progenitor Cell Pre-Enrichment Cocktail §	15027	Enrichment of CD34+ Cells: 25 ± 10 Fold	For labeling 40 mL of bone marrow
	Cord Blood and Whole Blood	RosetteSep™ Human Hematopoietic Progenitor Cell Enrichment Cocktail §	15026	29.0 ± 9.0% CD34+ cells	For labeling 40 mL of blood

*RF denotes the RoboSep™ compatible kit.
^§These kit are debulking kits, intended to be used with downstream applications such as flow sorting.

For evaluation of cells after isolation, you can use Anti-Human CD34 Antibody (Catalog #60013).

Additionally, STEMCELL Technologies also provides fresh and frozen Primary Human Hematopoietic Cells (i.e. mononuclear or CD34+ purified cells) from multiple sources.


Isolation of Mouse Hematopoietic Progenitor Cells:

CELL TYPE	SOURCE	PRODUCT NAME	CATALOG #	PURITY	CAPACITY
Hematopoietic Progenitor Cells	Bone Marrow	EasySep™ Mouse Hematopoietic Progenitor Cell Isolation Kit	19856/19856RF*	60.0-84.0% Lin- cells	For labelling up to 1x109 cells
SCA-1	Bone Marrow	EasySep™ Mouse SCA1 Positive Selection Kit	18756/18756RF	80.0 – 98.0%	For labeling 2x109 cells
CD117 (cKIT)	Bone Marrow	EasySep™ Mouse CD117 (cKIT) Positive Selection Kit	18757/18757RF	88.0 – 95.0%	For labeling 2x109 cells

*RF denotes the RoboSep™ compatible kit.

Hematopoietic Stem Cells (HSCs) and closely related primitive progenitors in mice are distinguished from the majority of the cells in hematopoietic tissues by their lack of expression of markers specific to maturing blood cells (i.e. CD3, CD11b, CD45R/B220, GR1, TER119). In many mouse strains, HSCs are positive for SCA1 (Ly-6A/E) and cKIT (CD117) (Lin-SCA1+cKIT+phenotype) (Spangrude, Heimfeld, et al. 1988; Uchida & Weissman 1992).

For further questions regarding these products, you can contact Technical Support at techsupport@stemcell.com.