In this talk from the American Society of Gene & Cell Therapy 2021 meeting, Selena Hallahan, Product Manager, and Crystal Chau, Senior Research Associate, discuss novel methods for identifying treatments for sickle cell diseases. Learn how you can generate high yields of hPSC-derived erythroblasts that can be used in disease modeling and drug discovery applications.
Below are a few questions related to hPSC-derived erythroid progenitors that were asked during the talk:
1. What is the best maintenance media to be used with the STEMdiff™ Erythroid Kit?
Both mTeSR™ Plus and TeSR™-E8™ work well as maintenance media for this kit. mTeSR™1, which is now manufactured under relevant cGMPs, can also be used with this kit.
2. Can blood cell-derived hPSCs be used as a starting source for the STEMdiff™ Erythroid Kit?
Yes, blood cell-derived hPSCs are compatible with this kit.
3. Do cells differentiated using the STEMdiff™ Erythroid Kit generate colonies in the CFU assay?
Yes, cells harvested at Day 10 of the STEMdiff™ Erythroid Kit protocol generate both myeloid (CFU-G, CFU-M, CFU-GM) and erythroid colonies (CFU-E, BFU-E). Cells harvested at Day 24 (end of the STEMdiff™ Erythroid Kit protocol) mainly produce CFU-E and BFU-E in a CFU assay.
4. What is the ideal cryopreservation medium for storing hPSC-derived erythroid progenitors?
The cells generated using STEMdiff™ Erythroid Kit can be cryopreserved at either Day 10 or Day 24 of your culture. They can be frozen down in FreSR™-S, CryoStor® CS10, and FBS containing 10% DMSO. FreSR™-S provides the best visibility and recovery, and the cellular functions and characteristics are well preserved using this freezing medium.
5. Can the erythroid progenitors derived using the STEMdiff™ Erythroid Kit be genetically modified?
Yes, cells generated using this kit can undergo genome editing.
6. How can you generate hematopoietic progenitors from hPSCs?
Human ES or iPS cells can be differentiated into hematopoietic progenitor cells using the STEMdiff™ Hematopoietic Kit.