Considerations for Choosing the Right Product to Isolate Hematopoietic Progenitors
Technical tip from our dedicated team of Product and Scientific Support specialists
STEMCELL's fast, easy and column-free cell isolation products, EasySep™, RoboSep™, RosetteSep™, and SepMate™ can be used to isolate hematopoietic stem and progenitor cells (HSPCs) with high purity and recovery. Cells can be used immediately following isolation for downstream functional and biological studies.
Isolation of Human CD34+ Hematopoietic Progenitor Cells
CD34+ Cells
*RF denotes the RoboSep™ compatible kit.
Hematopoietic Progenitor Cells
§These kit are debulking kits, intended to be used with downstream applications such as flow sorting.
For evaluation of cells after isolation, you can use Anti-Human CD34 Antibody (Catalog #60013).
Isolation of Mouse Hematopoietic Progenitor Cells
Hematopoietic Progenitor Cells
*RF denotes the RoboSep™ compatible kit.
SCA-1
CD117 (cKIT)
Hematopoietic Stem Cells (HSCs) and closely related primitive progenitors in mice are distinguished from the majority of the cells in hematopoietic tissues by their lack of expression of markers specific to maturing blood cells (i.e. CD3, CD11b, CD45R/B220, GR1, TER119). In many mouse strains, HSCs are positive for SCA1 (Ly-6A/E) and cKIT (CD117) (Lin-SCA1+cKIT+phenotype) (Spangrude, Heimfeld, et al. 1988; Uchida & Weissman 1992).
For further questions regarding these products, you can contact Technical Support at techsupport@stemcell.com.
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