Isolating Hematopoietic Progenitors Using EasySep™, RoboSep™, RosetteSep™ and SepMate™

STEMCELL's fast, easy and column-free cell isolation products EasySep™, RoboSep™, RosetteSep™ and SepMate™ can be used to isolate hematopoietic stem and progenitor cells (HSPCs) with high purity and recovery. Cells can be used immediately following isolation for downstream functional and biological studies.

Isolation of Human CD34+ Hematopoietic Progenitor Cells:

CELL TYPE

SOURCE

PRODUCT NAME

CATALOG #

PURITY

CAPACITY

CD34 + Cells

Mononuclear cells from mobilized peripheral blood, cord blood or bone marrow and hESC/hiPSC-derived hematopoietic cells

EasySep™ Human CD34 Positive Selection Kit II

17856/17856RF*

92.4 - 94.6%

For labeling up to 5x109 cells

Whole Blood

Complete Kit for Human Whole Blood CD34+ Cells

15086/15086RF

79.0-95.0%

For labeling 120 mL of whole blood

Cord Blood

EasySep™ Human Cord Blood CD34 Positive Selection Kit II

17896/17896RF

91.0± 9.0%

For labeling 100 mL of cord blood

Hematopoietic Progenitor Cells

Cord Blood

EasySep™ Human Progenitor Enrichment Kit with Platelet Depletion

19356/19356RF

50.0 – 75.0%

CD34+ cells

For labeling up to 1 x 109 cells

Bone Marrow

RosetteSep™ Human Bone Marrow Progenitor Cell Pre-Enrichment Cocktail§

15027

Enrichment of CD34+ Cells: 25 ± 10 Fold

For labeling 40 mL of bone marrow

Cord Blood and Whole Blood

RosetteSep™ Human Hematopoietic Progenitor Cell Enrichment Cocktail§

15026

29.0 ± 9.0%

CD34+ cells

For labeling 40 mL of blood

*RF denotes the RoboSep™ compatible kit.
§These kit are debulking kits, intended to be used with downstream applications such as flow sorting.

For evaluation of cells after isolation, you can use Anti-Human CD34 Antibody (Catalog #60013).

Additionally, STEMCELL Technologies also provides fresh and frozen Primary Human Hematopoietic Cells (i.e. mononuclear or CD34+ purified cells) from multiple sources.


Isolation of Mouse Hematopoietic Progenitor Cells:

CELL TYPE

SOURCE

PRODUCT NAME

CATALOG #

PURITY

CAPACITY

Hematopoietic Progenitor Cells

Bone Marrow

EasySep™ Mouse Hematopoietic Progenitor Cell Isolation Kit

19856/19856RF*

60.0-84.0%

Lin- cells

For labelling up to 1x109 cells

SCA-1

Bone Marrow

EasySep™ Mouse SCA1 Positive Selection Kit

18756/18756RF

80.0 – 98.0%

 

For labeling 2x109 cells

CD117 (cKIT)

Bone Marrow

EasySep™ Mouse CD117 (cKIT) Positive Selection Kit

18757/18757RF

88.0 – 95.0%

For labeling 2x109 cells

*RF denotes the RoboSep™ compatible kit.

Hematopoietic Stem Cells (HSCs) and closely related primitive progenitors in mice are distinguished from the majority of the cells in hematopoietic tissues by their lack of expression of markers specific to maturing blood cells (i.e. CD3, CD11b, CD45R/B220, GR1, TER119). In many mouse strains, HSCs are positive for SCA1 (Ly-6A/E) and cKIT (CD117) (Lin-SCA1+cKIT+phenotype) (Spangrude, Heimfeld, et al. 1988; Uchida & Weissman 1992).

For further questions regarding these products, you can contact Technical Support at techsupport@stemcell.com.