Educational Materials (7)
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Data and Publications
Figure 1. High Purity of T Cells Following Isolation with RoboSep™-C
T cells were isolated from Fresh Human Peripheral Blood Leukopaks (between 2.5 - 20 x 109 starting cells) by negative selection. RoboSep™-C isolation yielded high purity of T cells, CD4+ T cells, and CD8+ T cells. Data shown as mean ± SD; n = 12 - 16.
Figure 2. Cell Isolation with RoboSep™-C Yields High Recovery of T Cells from a Leukopak
T cells were isolated from Fresh Human Peripheral Blood Leukopaks by negative selection. RoboSep™-C isolation yielded a high recovery of a) T cells, b) CD4+ T cells, and c) CD8+ T cells. Data shows the number of target cells recovered per 10 x 109 starting cells (mean ± SD; n= 12 - 16).
Figure 3. Cells Isolated Using RoboSep™-C Show Comparable Viability to Starting Samples
T cells were isolated from Fresh Human Peripheral Blood Leukopaks using RoboSep™-C isolation kits. Pre- and post-isolation samples were stained with the cell viability dye DRAQ7™ and appropriate cell surface markers and were assessed by flow cytometry. Cells isolated using RoboSep™-C showed no significant difference in viability (% DRAQ7-negative events) compared to the starting samples. Data shown as mean ± SD; n = 12 - 16.
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