UM729

Pyrimido-indole derivative that enhances HSC self-renewal in vitro

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UM729

Pyrimido-indole derivative that enhances HSC self-renewal in vitro

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250 µg

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Pyrimido-indole derivative that enhances HSC self-renewal in vitro

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Overview

UM729 is a pyrimido-[4,5-b]-indole derivative which enhances the self-renewal of human hematopoietic stem cells in vitro. UM729 does not inhibit the aryl hydrocarbon receptor (AHR) pathway, but has been shown to collaborate with AHR antagonists in preventing differentiation of acute myeloid leukemia (AML) cells in culture.

MAINTENANCE AND SELF-RENEWAL
· Enhances human hematopoietic stem cell self-renewal in vitro (Fares et al.).

CANCER RESEARCH:
· Collaborates with StemRegenin 1 (SR1) in preventing differentiation of AML cells in culture (Pabst et al.).

For clinical use of UM729, please contact ExCellThera.

Cancer Cells and Cell Lines, Hematopoietic Stem and Progenitor Cells, Leukemia/Lymphoma Cells

Human

Expansion, Maintenance

Cancer, Stem Cell Biology

Not applicable

C₂₀H₂₅N₅O₂ · X HCl [X H₂O]

≥ 95%

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type

Product Name

Catalog #

Lot #

Language

Document Type

Product Information Sheet

Product Name

UM729

Catalog #

72332

Lot #

All

Language

English

Document Type

Safety Data Sheet

Product Name

UM729

Catalog #

72332

Lot #

All

Language

English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area

Workflow Stages

Workflow Stages for Hematopoietic Stem and Progenitor Cell Research

Cell Sourcing and Isolation

Expansion and Differentiation

Analysis

Workflow Stages for Hematopoietic Cell Therapy Development

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Resources and Publications

Cord blood expansion. Pyrimidoindole derivatives are agonists of human hematopoietic stem cell self-renewal. Fares I et al. Science (New York, N.Y.) 2014 SEP

Abstract

The small number of hematopoietic stem and progenitor cells in cord blood units limits their widespread use in human transplant protocols. We identified a family of chemically related small molecules that stimulates the expansion ex vivo of human cord blood cells capable of reconstituting human hematopoiesis for at least 6 months in immunocompromised mice. The potent activity of these newly identified compounds, UM171 being the prototype, is independent of suppression of the aryl hydrocarbon receptor, which targets cells with more-limited regenerative potential. The properties of UM171 make it a potential candidate for hematopoietic stem cell transplantation and gene therapy.

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Identification of small molecules that support human leukemia stem cell activity ex vivo. Pabst C et al. Nature methods 2014 APR

Abstract

Leukemic stem cells (LSCs) are considered a major cause of relapse in acute myeloid leukemia (AML). Defining pathways that control LSC self-renewal is crucial for a better understanding of underlying mechanisms and for the development of targeted therapies. However, currently available culture conditions do not prevent spontaneous differentiation of LSCs, which greatly limits the feasibility of cell-based assays. To overcome these constraints we conducted a high-throughput chemical screen and identified small molecules that inhibit differentiation and support LSC activity in vitro. Similar to reports with cord blood stem cells, several of these compounds suppressed the aryl-hydrocarbon receptor (AhR) pathway, which we show to be inactive in vivo and rapidly activated ex vivo in AML cells. We also identified a compound, UM729, that collaborates with AhR suppressors in preventing AML cell differentiation. Together, these findings provide newly defined culture conditions for improved ex vivo culture of primary human AML cells.

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Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.

UM729

Pyrimido-indole derivative that enhances HSC self-renewal in vitro

UM729

Pyrimido-indole derivative that enhances HSC self-renewal in vitro

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Overview

Protocols and Documentation

Applications

Resources and Publications

Educational Materials (7)

Publications (2)

Abstract

Abstract

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