UM729
Pyrimido-indole derivative that enhances HSC self-renewal in vitro
Overview
UM729 is a pyrimido-[4,5-b]-indole derivative which enhances the self-renewal of human hematopoietic stem cells in vitro. UM729 does not inhibit the aryl hydrocarbon receptor (AHR) pathway, but has been shown to collaborate with AHR antagonists in preventing differentiation of acute myeloid leukemia (AML) cells in culture.
MAINTENANCE AND SELF-RENEWAL
· Enhances human hematopoietic stem cell self-renewal in vitro (Fares et al.).
CANCER RESEARCH:
· Collaborates with StemRegenin 1 (SR1) in preventing differentiation of AML cells in culture (Pabst et al.).
MAINTENANCE AND SELF-RENEWAL
· Enhances human hematopoietic stem cell self-renewal in vitro (Fares et al.).
CANCER RESEARCH:
· Collaborates with StemRegenin 1 (SR1) in preventing differentiation of AML cells in culture (Pabst et al.).
Alternative Names
UM-729
Cell Type
Cancer Cells and Cell Lines, Hematopoietic Stem and Progenitor Cells, Leukemia/Lymphoma Cells
Species
Human
Application
Expansion, Maintenance
Area of Interest
Cancer, Stem Cell Biology
CAS Number
Not applicable
Chemical Formula
C₂₀H₂₅N₅O₂ · X HCl [X H2O]
Molecular Weight
367.4 g/mol (free base)
Purity
≥ 95%
Related Products
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Scientific Resources
Product Documentation
| Document Type | Product Name | Catalog # | Lot # | Language |
|---|---|---|---|---|
|
Document Type
Product Information Sheet
|
Product Name
UM729
|
Catalog #
72332 |
Lot #
All |
Language
English |
|
Document Type
Safety Data Sheet
|
Product Name
UM729
|
Catalog #
72332 |
Lot #
All |
Language
English |
Educational Materials (9)
Brochure
Small Molecules for Cancer Research
Brochure
Tools for Cancer Research
Brochure
UM171 & UM729: Novel Small Molecules for the Ex Vivo Expansion of Human Hematopoietic Stem Cells
Brochure
Small Molecules for Stem Cell Research
Brochure
Hematopoietic Stem and Progenitor Cells - Products for Your Research
Technical Bulletin
Culturing Leukemic Stem & Progenitor Cells with StemSpan™ Medium
Webinar
1:06:52
New Tools for the Ex Vivo Expansion of Human Hematopoietic Stem and Progenitor Cells
Webinar
45:17
Troubleshooting In Vitro Expansion of Leukemic Cells
Mini Review
Small Molecules
Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for
Hematopoietic Stem and Progenitor Cell Research
Workflow Stages for
Hematopoietic Cell Therapy Research
Data and Publications
Publications (2)
Science (New York, N.Y.) 2014 SEP
Cord blood expansion. Pyrimidoindole derivatives are agonists of human hematopoietic stem cell self-renewal.
Abstract
Abstract
The small number of hematopoietic stem and progenitor cells in cord blood units limits their widespread use in human transplant protocols. We identified a family of chemically related small molecules that stimulates the expansion ex vivo of human cord blood cells capable of reconstituting human hematopoiesis for at least 6 months in immunocompromised mice. The potent activity of these newly identified compounds, UM171 being the prototype, is independent of suppression of the aryl hydrocarbon receptor, which targets cells with more-limited regenerative potential. The properties of UM171 make it a potential candidate for hematopoietic stem cell transplantation and gene therapy.
Nature methods 2014 APR
Identification of small molecules that support human leukemia stem cell activity ex vivo.
Abstract
Abstract
Leukemic stem cells (LSCs) are considered a major cause of relapse in acute myeloid leukemia (AML). Defining pathways that control LSC self-renewal is crucial for a better understanding of underlying mechanisms and for the development of targeted therapies. However, currently available culture conditions do not prevent spontaneous differentiation of LSCs, which greatly limits the feasibility of cell-based assays. To overcome these constraints we conducted a high-throughput chemical screen and identified small molecules that inhibit differentiation and support LSC activity in vitro. Similar to reports with cord blood stem cells, several of these compounds suppressed the aryl-hydrocarbon receptor (AhR) pathway, which we show to be inactive in vivo and rapidly activated ex vivo in AML cells. We also identified a compound, UM729, that collaborates with AhR suppressors in preventing AML cell differentiation. Together, these findings provide newly defined culture conditions for improved ex vivo culture of primary human AML cells.
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
