StemSpan™ Myeloid Expansion Supplement II (100X)

Serum-free culture supplement for expansion and differentiation of human monocytes

More Views

StemSpan™ Myeloid Expansion Supplement II (100X)

Serum-free culture supplement for expansion and differentiation of human monocytes

1 mL
Catalog #02694
597 USD

Overview

StemSpan™ Myeloid Expansion Supplement II (100X) contains a combination of recombinant human cytokines (SCF, TPO, Flt3L, M-CSF, and GM-CSF) and other supplements formulated to selectively promote the expansion and monocytic differentiation of CD34+ cells isolated from human cord blood (CB) samples. StemSpan™ Myeloid Expansion Supplement II is intended for use in combination with StemSpan™ SFEM II (Catalog #09605) serum-free expansion medium.

When added to serum-free medium, StemSpan™ Myeloid Expansion Supplement II typically promotes the production of hundreds of CD14+ monocytes per input CD34+ cell in 14-day liquid cultures initiated with CD34+ human CB cells. See data tab for more details.


This product is recommended for:
• Research into the regulation of monocyte development
• Development of procedures to expand monocytes and macrophages in culture
Advantages:
Advantages:
• Promotes the production of hundreds of CD14+ monocytes per input CD34+ human CB cell in 14-day liquid cultures.
• Supplied as a 100X concentrate. After thawing and mixing, the tube contents can be added directly to any hematopoietic cell expansion medium.
Contains:
• Recombinant human fms-like tyrosine kinase 3 ligand (Flt3L)
• Recombinant human stem cell factor (SCF)
• Recombinant human thrombopoietin (TPO)
• Recombinant human macrophage colony-stimulating factor (M-CSF)
• Recombinant human granulocyte/macrophage colony-stimulating factor (GM-CSF)
• Supplements
Subtype:
Supplements
Cell Type:
Hematopoietic Stem and Progenitor Cells; Monocytes; Myeloid Cells
Species:
Human
Application:
Cell Culture; Differentiation; Expansion
Brand:
StemSpan
Area of Interest:
Stem Cell Biology; Transplantation Research
Formulation:
Serum-Free

Scientific Resources

Educational Materials

(4)

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Figure 1. Production of CD14+ Monocytes by the Expansion and Lineage-Specific Differentiation of Human CB CD34+ Cells Cultured in StemSpan™ SFEM II Containing Myeloid Expansion Supplement II

Flow cytometry dot plots for 14-day cultures of human CB CD34+ cells in StemSpan™ SFEM II containing Myeloid Expansion Supplement II. The plots show expression of CD34 and CD45 (A) before and (B) after culture, and (C) expression of CD13 and CD14 on the expanded cells. The frequency of CD34+ cells declined from 87% before culture to less than 1% after 14 days. CD14+ monocytes increased from < 5% on day 0 to 90% by day 14.

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.