StemSpan™ Myeloid Expansion Supplement II (100X)

Serum-free culture supplement for expansion and differentiation of human monocytes
StemSpan™ Myeloid Expansion Supplement II (100X)

Serum-free culture supplement for expansion and differentiation of human monocytes

1 mL
Catalog # 02694
678 USD

Overview

StemSpan™ Myeloid Expansion Supplement II (100X) contains a combination of recombinant human cytokines formulated to selectively promote the expansion and differentiation of human myeloid progenitor cells, generating large numbers of CD14+ monocytes from CD34+ cells isolated from human cord blood (CB) samples.

StemSpan™ Myeloid Expansion Supplement II (100X) is intended for use in combination with any of the following StemSpan™ media:
• StemSpan™ SFEM (Catalog #09600)
• StemSpan™ SFEM II (Catalog #09605)
• StemSpan™-XF (Catalog #100-0073)
• StemSpan™-AOF (Catalog #100-0130)

When added to serum-free medium, StemSpan™ Myeloid Expansion Supplement II typically promotes the production of hundreds of CD14+ monocytes per input CD34+ cell in 14-day liquid cultures initiated with CD34+ human CB cells. See data tab for more details.
Advantages
Advantages:
• Promotes the production of hundreds of CD14+ monocytes per input CD34+ human CB cell in 14-day liquid cultures.
• Supplied as a 100X concentrate. After thawing and mixing, the tube contents can be added directly to any hematopoietic cell expansion medium.
Contains
• Recombinant human fms-like tyrosine kinase 3 ligand (Flt3L)
• Recombinant human stem cell factor (SCF)
• Recombinant human thrombopoietin (TPO)
• Recombinant human macrophage colony-stimulating factor (M-CSF)
• Recombinant human granulocyte/macrophage colony-stimulating factor (GM-CSF)
• Supplements
Subtype
Supplements
Cell Type
Hematopoietic Stem and Progenitor Cells, Monocytes, Myeloid Cells
Species
Human
Application
Cell Culture, Differentiation, Expansion
Brand
StemSpan
Area of Interest
Stem Cell Biology, Transplantation Research
Formulation
Serum-Free

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
StemSpan™ Myeloid Expansion Supplement II (100X)
Catalog #
02694
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
StemSpan™ Myeloid Expansion Supplement II (100X)
Catalog #
02694
Lot #
All
Language
English

Educational Materials (5)

Brochure
StemSpan™ Media and Supplements for the Expansion and Differentiation of Granulocytes and Monocytes
Brochure
Hematopoietic Stem and Progenitor Cells - Products for Your Research
Brochure
StemSpan™: Defined Media and Supplements for Hematopoietic Cell Expansion
Webinar
Online Immunology Journal Club: New Strategies to Target Macrophages in Cancer
30:07
Online Immunology Journal Club: New Strategies to Target Macrophages in Cancer
Mini Review
Hematopoietic Stem and Progenitor Cells (HSPCs): Isolation, Culture, and Assays

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Figure 1. Production of CD14+ Monocytes by the Expansion and Lineage-Specific Differentiation of Human CB CD34+ Cells Cultured in StemSpan™ SFEM II Containing Myeloid Expansion Supplement II

Flow cytometry dot plots for 14-day cultures of human CB CD34+ cells in StemSpan™ SFEM II containing Myeloid Expansion Supplement II. The plots show expression of CD34 and CD45 (A) before and (B) after culture, and (C) expression of CD13 and CD14 on the expanded cells. The frequency of CD34+ cells declined from 87% before culture to less than 1% after 14 days. CD14+ monocytes increased from < 5% on day 0 to 90% by day 14.

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