ImmunoCult™ Dendritic Cell Culture Kit

For differentiation of human monocytes into dendritic cells
ImmunoCult™ Dendritic Cell Culture Kit

For differentiation of human monocytes into dendritic cells

1 Kit
Catalog # 10985
600 USD
ImmunoCult™-ACF Dendritic Cell Medium

Medium for the culture and differentiation of human monocytes into dendritic cells and their maturation

500 mL
Catalog # 10986
568 USD
ImmunoCult™-ACF Dendritic Cell Medium

Medium for the culture and differentiation of human monocytes into dendritic cells and their maturation

100 mL
Catalog # 10987
162 USD
ImmunoCult™-ACF Dendritic Cell Differentiation Supplement

Supplement for the differentiation of human monocytes into dendritic cells

1 mL
Catalog # 10988
317 USD
ImmunoCult™ Dendritic Cell Maturation Supplement

Supplement for the maturation of human monocyte-derived dendritic cells

0.5 mL
Catalog # 10989
405 USD

Overview

ImmunoCult™-ACF Dendritic Cell Medium and Supplements, sold individually or as a kit, have been developed for the in vitro culture and differentiation of human monocytes into dendritic cells (DCs).

ImmunoCult™-ACF Dendritic Cell Medium is a serum-free and animal component-free medium optimized for the in vitro culture and differentiation of human monocytes into DCs.

ImmunoCult™-ACF Dendritic Cell Differentiation Supplement contains a combination of animal component-free recombinant human cytokines formulated to support the differentiation of immature DCs from human monocytes. The ImmunoCult™ Dendritic Cell Maturation Supplement has been formulated to support maturation of immature DCs to mature DCs.
Advantages
• Animal component-free and serum-free medium
• No need to supplement the medium with serum
• Optimized for differentiation of CD14+ monocytes into immature and subsequently mature DCs
• High yields of mature DCs with the desired phenotype
• Mature DCs are functional, produce the pro-inflammatory cytokine IL-12, and induce T cell proliferation
Components
• ImmunoCult™-ACF Dendritic Cell Medium, 100 mL (Catalog #10987)
• ImmunoCult™-ACF Dendritic Cell Differentiation Supplement, 1 mL (Catalog #10988)
• ImmunoCult™ Dendritic Cell Maturation Supplement, 0.5 mL (Catalog #10989)
Contains
ImmunoCult™-ACF Dendritic Cell Medium contains only recombinant proteins and synthetic components
Subtype
Specialized Media, Supplements
Cell Type
Dendritic Cells, Monocytes
Species
Human
Application
Cell Culture, Differentiation
Brand
ImmunoCult
Area of Interest
Immunology
Formulation
Animal Component-Free, Serum-Free

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
ImmunoCult™ Dendritic Cell Culture Kit
Catalog #
10985
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
ImmunoCult™-ACF Dendritic Cell Medium
Catalog #
10986, 10987
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
ImmunoCult™-ACF Dendritic Cell Differentiation Supplement
Catalog #
10988
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
ImmunoCult™ Dendritic Cell Maturation Supplement
Catalog #
10989
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
ImmunoCult™ Dendritic Cell Culture Kit
Catalog #
10985
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
ImmunoCult™ Dendritic Cell Culture Kit
Catalog #
10985
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
ImmunoCult™ Dendritic Cell Culture Kit
Catalog #
10985
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
ImmunoCult™-ACF Dendritic Cell Medium
Catalog #
10986, 10987
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
ImmunoCult™-ACF Dendritic Cell Differentiation Supplement
Catalog #
10988
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
ImmunoCult™ Dendritic Cell Maturation Supplement
Catalog #
10989
Lot #
All
Language
English

Educational Materials (7)

Brochure
Human Monocyte Research Product Workflow
Brochure
Tools For Your Immunology Research
Brochure
Tools to Streamline your Dendritic Cell Research
Wallchart
Antigen Processing and Presentation
Wallchart
Human Immune Cytokines
Video
How to Isolate Cells with EasySep™ Column-Free Cell Separation Technology
1:23
How to Isolate Cells with EasySep™ Column-Free Cell Separation Technology
Scientific Poster
An Animal Component-Free, Serum-Free Culture Method for Generation of Human Dendritic Cells

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Start: 54% CD4+CXCR3-CCR6+ T Cells

Figure 1. Protocol Diagram.

Mature DCs were generated by culturing EasySep™ isolated monocytes at 1 x 106 cells/mL in ImmunoCult™-ACF Dendritic Cell Medium (Catalog #10987) with added ImmunoCult™-ACF Dendritic Cell Differentiation Supplement (Catalog #10988). At day 3, the medium with differentiation supplement was replaced and cells were incubated for 2 more days. At day 5, without changing the medium, ImmunoCult™ Dendritic Cell Maturation Supplement (Catalog #10989) was added to the culture. At day 7, fully mature DCs were harvested for downstream applications.

Start: 54% CD4+CXCR3-CCR6+ T Cells

Figure 2. Mature DCs generated with ImmunoCult™-ACF Dendritic Cell Medium with Supplements show desired phenotype.

EasySep™ isolated monocytes were cultured and differentiated into mature DCs as described in Figure 1. (A) The percentage of CD14 and CD83 expression in cells at day 7 (mature DCs) was determined by flow cytometry. At day 7, a total of 93 ± 5% of the cells expressed the mature DC marker CD83 and only 1 ± 1% of cells still expressed the monocyte marker CD14 (mean ± SD, n=39). Yield of mature DCs was determined by count of total viable cells at day 7 relative to the count of viable monocytes used for initial culture at day 0. At day 7, the yield of viable mature DCs corresponded to 45 ± 25% (mean ± SD, n=39). (B) Immature DCs were cultured as described in Figure 1. At day 5, cells were cultured with maturation supplement for 2 days (mature DCs) or without maturation supplement (immature DCs). Supernatant was collected at day 7 and IL-12p70 levels were determined by ELISA. Concentrations of IL-12p70 in supernatant of mature and immature DCs were 361 ± 81 and 5 ± 2 pg/mL, respectively (mean ± SEM, n=27).

Start: 54% CD4+CXCR3-CCR6+ T Cells

Figure 3. Mature DCs generated with ImmunoCult™-ACF Dendritic Cell Medium and Supplements induce T cell proliferation.

Mature DCs generated with ImmunoCult™-ACF Dendritic Cell Medium and Supplements (ImmunoCult) or other serum-free competitor media (competitor 1 and 2) and corresponding supplements when applicable (competitor 2), were cultured in ImmunoCult™-XF T Cell Expansion Medium with 1 x 105 CFSE labeled (A) allogeneic CD3+ T cells (MLR assay) or (B) autologous CD8+ T cells (antigen-specific T cell response). (A) Cells were cultured at a DC:T cell ratio of 1:25. (B) Prior to culture with T cells, immature DCs were loaded with HLA Class I peptides derived from the human Cytomegalovirus, Epstein-Barr Virus and Influenza Virus (CEF peptide pool) and stimulated with maturation supplement for 2 days. Cells were cultured at a DC:T cell ratio of 1:4 or 1:10. (A,B) CFSE labeled T cells were incubated in media alone (negative control) or with ImmunoCult™ Human CD3/CD28 T Cell Activator (positive control). After 5-7 days in culture the number of dividing T cells ( CD3+CFSElo) was assessed by flow cytometry (mean ± SEM) (A) n=5 (B) n=4 (competitor 1 and 2, n=3). Mature DCs generated in ImmunoCult™-ACF Dendritic Cell Medium induced proliferation of allogeneic and antigen-specific T cells similar to DCs generated in either competitor media. Competitors 1 and 2, include in no particular order, CellGro DC Medium (CellGenix) and PromoCell DC Generation Medium DXF.

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