STEMdiff™ Cardiomyocyte Dissociation Kit
For dissociation of hPSC-derived cardiomyocytes
Required Products
-
Trypan BlueReagent for counting viable mammalian cells
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D-PBS (Without Ca++ and Mg++)Dulbecco’s phosphate-buffered saline without calcium and magnesium
Overview
STEMdiff™ Cardiomyocyte Dissociation Kit includes STEMdiff™ Cardiomyocyte Dissociation Medium and STEMdiff™ Cardiomyocyte Support Medium. STEMdiff™ Cardiomyocyte Dissociation Medium can be used to harvest cardiomyocytes that have been differentiated from human pluripotent stem cells (hPSCs) using STEMdiff™ Ventricular Cardiomyocyte Differentiation Kit (Catalog #05010) or STEMdiff™ Atrial Cardiomyocyte Differentiation Kit (Catalog #100-0215) and maintained in STEMdiff™ Cardiomyocyte Maintenance Medium (Catalog #05010/05020). STEMdiff™ Cardiomyocyte Support Medium reduces stress on these cardiomyocytes during harvesting and replating, maintaining their viability and functional capacity for downstream applications and analyses.
Advantages
• Maintains the integrity of hPSC-derived cardiomyocytes with greater than 80% cell viability.
• Simple and robust method for harvesting and replating hPSC-derived cardiomyocytes
• Simple and robust method for harvesting and replating hPSC-derived cardiomyocytes
Components
- STEMdiff™ Cardiomyocyte Dissociation Medium, 50 mL
- STEMdiff™ Cardiomyocyte Support Medium, 250 mL
Subtype
Specialized Media
Cell Type
Cardiomyocytes, PSC-Derived
Species
Human
Application
Cell Culture
Brand
STEMdiff
Area of Interest
Stem Cell Biology
Related Products
Related Products
Scientific Resources
Product Documentation
| Document Type | Product Name | Catalog # | Lot # | Language |
|---|---|---|---|---|
|
Document Type
Product Information Sheet
|
Product Name
STEMdiff™ Cardiomyocyte Dissociation Kit
|
Catalog #
05025 |
Lot #
All |
Language
English |
|
Document Type
Safety Data Sheet 1
|
Product Name
STEMdiff™ Cardiomyocyte Dissociation Kit
|
Catalog #
05025 |
Lot #
All |
Language
English |
|
Document Type
Safety Data Sheet 2
|
Product Name
STEMdiff™ Cardiomyocyte Dissociation Kit
|
Catalog #
05025 |
Lot #
All |
Language
English |
Educational Materials (4)
Brochure
STEMdiff™ Cardiomyocyte Differentiation Kit
Webinar
43:45
Modeling Arrhythmias Using hPSC-Derived Cardiomyocytes and Tracking Their Excitability
Scientific Poster
Efficient Production and Processing of Cardiomyocytes from Human Pluripotent Stem Cells Using STEMdiff™ Cardiomyocyte Products
Scientific Poster
Simple Label-free Monitoring of Robust Human Pluripotent Stem Cell Differentiation to Cardiomyocytes with the Maestro MEA System and STEMdiff™ Cardiomyocyte Products
Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for
Human Pluripotent Stem Cell Research
Workflow Stages for
Cardiac and Skeletal Muscle Research
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
