Standardization of the Hematopoietic Progenitor Assay Training Course
Two-day hands-on training on the CFU Assay for human cord blood and bone marrow samples
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Overview
Gain hands-on experience in the set-up, identification, and enumeration of hematopoietic progenitor cells using MethoCult™, the gold standard media for hematopoietic colony-forming unit (CFU) assays. Participants working with human cord blood, bone marrow, or mobilized peripheral blood samples will gain expertise in the enumeration of hematopoetic colonies through comparative counting exercises with the instructors. The STEMvision™ instrument designed specifically for the imaging and automated counting of colonies in CFU assays will be introduced.
Participants Will Receive
- Course booklet and electronic versions of instructional material
- Tote bag with sample-size products (where applicable)
- Certificate following successful completion of the course
- Lunch and refreshments on each day and dinner hosted on one of the evenings
STEMCELL Technologies
1618 Station Street
Vancouver, CA V6A 1B6
New England Cord Blood Bank
500 Donald Lynch Blvd
Marlborough, MA
01752 United States
STEMCELL Technologies UK Ltd.
Building 7100, Cambridge Research Park,
Beach Drive, Waterbeach
Cambridge, UK, CB25 9TL
Experimental Transfusion Medicine Dresden
c/o DRK Blutspendedienst Nord-Ost gGmbH
Blasewitzer Str. 68/70
01307 Dresden, Germany
Speakers
Speakers will vary depending on course topic, date, and location.
STEMCELL Technologies
STEMCELL Technologies
STEMCELL Technologies
Agenda
Note that each day will end at approximately 4:30 pm and the course dinner taking place on one of the evenings.
Day One
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Welcome Presentation and Introductions
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Lecture: Introduction to Hematopoiesis and the Colony-Forming Unit (CFU) Assay
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Lecture 2: CFU Assay Set Up and Colony Identification
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Practical Session 1 : CFU Assay Set Up Procedure
- Incubator maintenance
- Sample processing with ErythroClear™ Red Blood Cell Depletion Kit
- CFU assay setup
- Demo sample thawing and cell counting
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Practical Session 2: Manual Colony Identification
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Practical Session 3: Comparative Colony Counting
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Wrap Up and Q&A
Day Two
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Lecture: Standardization Tools for the Hematopoietic CFU Assay
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Practical Session: Review of Manual Colony Identification Criteria and Comparative Counting
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Practical Session: Review and Analysis of Group Comparative Counts
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Lecture: Introduction to Automated and Standardized Colony Counting for the CFU Assay with STEMvision™
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Practical Session: Operations of STEMvision™Wrap up Session:
- Q&A
- Quiz
- Course Evaluation
- Certificate Presentation
Accommodation
Vancouver, CA
Boston, US
Cambridge, UK (Transport included from this location to and from training venue - Cambridge UK only)
Dresden, DE
Contact education@stemcell.com for information on special rates (when applicable).
Policy
Travel Visas
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CanadaUnited States
United Kingdom
Germany
Cancellation
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
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Resources and Publications
Educational Materials (4)
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