Human IFN-gamma ELISA Kit

For detection and measurement of human interferon-gamma

Human IFN-gamma ELISA Kit

For detection and measurement of human interferon-gamma

From: 837 USD
Catalog #
02002_C
For detection and measurement of human interferon-gamma

What's Included

  • Human IFN-gamma ELISA Kit, 2 plates (Catalog #02002)
    • Human IFN-γ ELISA Plate, 2x
    • Human IFN-γ Standard, 1 vial
    • Human IFN-γ Biotinylated Detection Antibody, 30 µL
    • SA-HRP, 30 µL
    • Standard Reconstitution Buffer C, 1 mL
    • Wash Buffer (20X), 120 mL
    • ELISA Diluent, 120 mL
    • SA-HRP Diluent, 25 mL
    • TMB Substrate, 25 mL
    • Stop Solution, 25 mL
    • Adhesive Plate Covers, 6x
 
  • Human IFN-gamma ELISA Kit, 10 plates (Catalog #02003)
    • Human IFN-γ ELISA Plate, 10x
    • Human IFN-γ Standard, 1 vial
    • Human IFN-γ Biotinylated Detection Antibody, 130 µL
    • SA-HRP, 130 µL
    • Standard Reconstitution Buffer C, 1 mL
    • Wash Buffer (20X), 5 x 120 mL
    • ELISA Diluent, 3 x 120 mL
    • SA-HRP Diluent, 120 mL
    • TMB Substrate, 120 mL
    • Stop Solution, 120 mL
    • Adhesive Plate Covers, 30x

Overview

The Human Interferon-gamma (IFN-γ) ELISA Kit is designed for the quantitative detection and measurement of human IFN-γ in biological fluids such as serum, plasma, and cell culture supernatants. IFN-γ is primarily secreted by T and NK cells, but is also produced by dendritic cells and macrophages in smaller amounts. IFN-γ is a pleiotropic cytokine that modulates a broad range of immunological functions including antimicrobial and antitumor activity, and increases the surface expression of MHC class I and II proteins.

The assay is based on the sandwich ELISA method, in which samples are added to ELISA strip plates pre-coated with capture antibodies specific for the cytokine. The captured cytokine is detected by addition of a biotinylated detection antibody, followed by streptavidin-horseradish peroxidase, which binds the biotinylated antibody. Addition of the chromogenic enzyme substrate 3,3’,5,5’ tetramethylbenzidine (TMB) results in a colored product with an intensity directly proportional to the concentration of cytokine in the sample. The concentration of the cytokine is determined by comparison to a serial dilution of the cytokine standard analyzed in parallel.
Subtype
Complete Kits
Cell Type
Dendritic Cells, Macrophages, NK Cells, T Cells
Species
Human
Area of Interest
Immunology

Scientific Resources

Product Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
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Catalog #
02002, 02003
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1000069999 and lower
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English
Catalog #
02002, 02003
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1000070000 and higher
Language
English
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Safety Data Sheet 1
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All
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English
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Safety Data Sheet 2
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English
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Safety Data Sheet 3
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Safety Data Sheet 5
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Safety Data Sheet 6
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Safety Data Sheet 9
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English
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All
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English

Educational Materials (2)

Product Applications

Data and Publications

Representative Standard Curve

• Reportable range: 3.2 - 1000 pg/mL. This is the concentration range in which measurement of the analyte can be done with the highest precision, accuracy, and linearity.
• Sensitivity: The limit of detection of this assay is 1 pg/mL. This is the analyte concentration with absorbance two standard deviations higher than the zero standard.
• Accuracy: The analyte standard of this ELISA was calibrated against NIAID* international standard Gxg01-902-535.
• Recovery: A mid-curve recovery of 85 - 91% was determined by spiking defined amounts of analyte standard into serum or plasma samples in repeated experiments.
• Precision: The intra-assay precision of this assay is 3.9% (CV). The inter-assay precision of this assay is 4.4% (CV).

*National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892-6612, USA.

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