Human Recombinant IL-10

Interleukin 10

Human Recombinant IL-10

Interleukin 10

From: 189 USD
Catalog #
78024_C
Interleukin 10

Overview

Interleukin 10 (IL-10) is the founding member of the IL-10 family of class II cytokines. All of the IL-10 cytokine family members have a four-helix bundle consisting of α-helical folds. Upon binding to its receptor, IL-10 activates signaling through JAK1 and STAT3. It is produced by dendritic cells, macrophages, and CD4+ T cells, as well as mast cells, NK cells, neutrophils, and B cells, under specific stimulating conditions (Saraiva & O'Garra). IL-10 can inhibit the expression of pro-inflammatory cytokines and promote healing processes, and is important for the function of regulatory T cells. IL-10 also enhances B cell proliferation, immunoglobulin secretion, and class II MHC expression, while IL-10 produced by macrophages inhibits activation of neighboring macrophages, thus allowing a level of self-regulation (Ouyang et al.).
Subtype
Cytokines
Alternative Names
B-TCGF, CSIF, Cytokine synthesis inhibitory factor, Interleukin-10, TGIF
Cell Type
Dendritic Cells, Hematopoietic Stem and Progenitor Cells, Myeloid Cells, Neurons, T Cells
Species
Human
Area of Interest
Immunology, Neuroscience, Stem Cell Biology
Molecular Weight
18.6 kDa
Purity
≥ 95%

Data Figures

(A) The biological activity of Human Recombinant IL-10 was tested by its ability to promote the proliferation of MC/9 cells. Cell proliferation was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the example above is less than 0.2 ng/mL.
(B) 5 μg of Human Recombinant IL-10 was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant IL-10 has a predicted molecular mass of 18 - 19 kDa.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
78024, 78024.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
78024, 78024.1
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Resources and Publications

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