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Data and Publications
(A) The biological activity of Human Recombinant BAFF was tested by its ability to promote mitosis of RPMI-8226 cells. Mitogenic activity
was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which
mitogenic activity is at 50% of maximum. The EC50 in the above example is less than 20 ng/mL.
(B) 1 μg of Human Recombinant BAFF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions visualized by Coomassie Blue staining. Human Recombinant BAFF has a predicted molecular mass of 17 kDa.