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Cell Storage Media

Freeze Your Cells with Our Optimized Cryopreservation Media

Storage and cryopreservation of cells and tissues are vital for your biological research workflow. Along with defined and serum-free cryopreservation media optimized for cells cultured in STEMCELL’s media, we offer a suite of cGMP-manufactured, protein-free and serum-free cryopreservation products that are designed to maintain high cell viability and maximize cell recovery after long-term storage.

cGMP-Manufactured Preservation Media Formulated with USP-Grade Components:

CryoStor® Freezing Media

Cell Types:

  • Cord blood and tissue, peripheral blood, mesenchymal stem cells, pluripotent stem cells, tissue samples and others

Recommended For:

  • Cryopreservation of hepatocytes, tissue samples, human peripheral blood, human mesenchymal stem cells, human embryonic and human induced pluripotent stem cells (human ES cells and iPS cells) and other extremely sensitive cell types

Features:

  • Designed to mitigate temperature-induced molecular stress responses during freezing and thawing

Formulation:

  • Pre-formulated with 2%, 5%, or 10% USP-grade DMSO
  • Manufactured under cGMP

BloodStor® Freezing Media

Cell Types:

  • Cord blood and tissue, peripheral blood and bone marrow

Recommended For:

  • Cryopreservation of stem cells and other cells isolated from umbilical cord blood, peripheral blood, bone marrow, and other biologics

Features:

  • Formulated to be compatible with many automated stem cell banking systems

Formulation:

  • BloodStor® 55-5 is preformulated with 55% (w/v) DMSO USP, 5% (w/v) Dextran-40 USP and water for injection (WFI) quality water
  • BloodStor® 100 contains 100% (w/v) DMSO USP
  • Manufactured under cGMP

HypoThermosol® FRS Preservation Media

Cell Types:

  • All cells and tissues including pluripotent stem cells, hematopoietic stem and progenitor cells, and mesenchymal stem cells

Recommended For:

  • Short-term storage and/or shipment of cells at 2 – 8°C rather than at cryogenic temperatures

Features:

  • Uniquely formulated to address the molecular-biological response of cells during the hypothermic preservation process

Formulation:

  • HypoThermosol® FRS Preservation Media is a serum-free, protein-free and animal component-free medium
  • Manufactured under cGMP
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Defined and Serum-Free Cryopreservation Media:

mFreSR™ and FreSR-S™ Cryopreservation Media

Cell Types:

  • Human embryonic stem (ES) and induced pluripotent stem (iPS) cells

Recommended For:

  • Cryopreservation of ES and iPS cells cultured in TeSR™ media

Features:

  • Higher thawing efficiencies than with conventional methods using serum1-4
  • FreSR™-S is optimized for cryopreservation of cells in single cell suspension

Formulation:

  • FreSR™-S is an animal component-free (ACF) medium, optimized for cryopreservation of cells in single cell suspension

MesenCult™-ACF Cryopreservation Medium

Cell Types:

  • Mesenchymal stem cells (MSCs)

Recommended For:

  • Cryopreservation of MSCs, including human MSCs previously cultured in MesenCult™-XF or MesenCult™-ACF media

Features:

  • Thawed MSCs have reproducibly high recovery rates and maintain MSC multipotency and expansion capacities

Formulation:

  • MesenCult™-ACF Freezing Medium is a defined, serum-free and animal component-free medium that contains DMSO

STEMdiff™ Neural Progenitor Freezing Medium

Cell Types:

  • Neural progenitor cells (NPCs) derived from human pluripotent stem cells

Recommended For:

  • Cryopreservation of NPCs generated from ES or iPS cells using STEMdiff™ Neural Induction Medium

Features:

  • NPCs can be frozen at any point post-neural induction with reproducibly high recovery rates
  • Post-thaw, NPCs display healthy morphology, express NPC markers and retain the potential to expand and differentiate into neurons, astrocytes and other neural cell types

Formulation:

  • STEMdiff™ Neural Progenitor Freezing Medium as a defined and serum-free medium containing DMSO
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References

  1. Fujioka T, et al. (2004) A simple and efficient cryopreservation method for primate embryonic stem cells. Int J Dev Biol 48(10): 1149-54
  2. Ha SY, et al. (2005) Cryopreservation of human embryonic stem cells without the use of a programmable freezer. Hum Reprod 20(7): 1779-85
  3. Ji L, et al. (2004) Cryopreservation of adherent human embryonic stem cells. Biotechnol Bioeng 88(3): 299-312
  4. Ware CB, et al. (2005) Controlled-rate freezing of human ES cells. Biotechniques 38(6): 879-80, 882-3
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