Cell Storage Media
Freeze Your Cells with Our Optimized Cryopreservation Media
cGMP-Manufactured Preservation Media Formulated with USP-Grade Components:
Defined and Serum-Free Cryopreservation Media:
Data

Figure 1. Immune Cells Cryopreserved in CryoStor®CS10 Show Reproducibly High Post-Thaw Cell Viability
CryoStor®CS10 effectively mitigates temperature-induced molecular cell stress responses to maximize post-thaw viability and recovery for a variety of immune cell types, including T cells (data not shown) and B cells. Here, human B cells from 6 different donors cryopreserved in CryoStor®CS10 show reproducibly high viability after thawing, as measured by Propidium Iodide staining (ranging from 94.3 - 97.9%).

Figure 2. Immune Cells Cryopreserved in CryoStor®CS10 Retain Functionality Post-Thaw
(A) Human peripheral blood Pan-T cells cryopreserved in CryoStor®CS10 were thawed and cultured with or without the addition of T cell activating factors. Cells from Donors 1-3 were cultured in RPMI Medium supplemented with 10% FBS, with (activated) or without (control) 40 ng/mL PMA and 1 ug/mL Ionomycin for 24 hours. Cells from Donors 4-5 were cultured in ImmunoCult™-XF T Cell Expansion Medium (Catalog #10981), with (activated) or without (control) ImmunoCult™ Human CD3/CD28 T Cell Activator (Catalog #10971) for 48 hours. Supernatants were collected from the cultures, and concentrations of secreted cytokines were determined using the Human IL-2 ELISA Kit (Catalog #02006). Activation by either PMA and Ionomycin or ImmunoCult™ Human CD3/CD28 T Cell Activator led to increased secretion of IL-2 compared to unstimulated control cultures. (B) Human B cells (Donors 6 - 11) cryopreserved in CryoStor®CS10 were thawed and activated with 1 µg/mL CD40 and 100 ng/mL IL-21 for 7 days. Supernatants were collected from the cultures and immunoglobulin G (IgG) production was measured using the Human IgG ELISA Antibody Pair Kit (Catalog #01994). Compared to unstimulated control cultures, B cell activation led to increased IgG secretion.

Figure 3. mFreSR™ Improves Thawing Efficiencies 5- to 10-Fold over Other Reported Methods
H9 hESCs were cryopreserved in mFreSR™ at the indicated passage number. Thawing efficiencies were analyzed by counting the number of surviving clumps after thawing.
References
- Fujioka T, et al. (2004) A simple and efficient cryopreservation method for primate embryonic stem cells. Int J Dev Biol 48(10): 1149-54
- Ha SY, et al. (2005) Cryopreservation of human embryonic stem cells without the use of a programmable freezer. Hum Reprod 20(7): 1779-85
- Ji L, et al. (2004) Cryopreservation of adherent human embryonic stem cells. Biotechnol Bioeng 88(3): 299-312
- Ware CB, et al. (2005) Controlled-rate freezing of human ES cells. Biotechniques 38(6): 879-80, 882-3