Animal component-free medium for cryopreserving ES and iPS cells as single cells
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Figure 1. High Viability and Recovery of Cells Stored in FreSR™-S
hPSCs cryopreserved as single cells using FreSR™-S have (A) higher post-thaw recovery (number of cells recovered / number of cells frozen) and (B) maintain higher viability (number of live cells / total number of cells) compared to competitor medium. All data values are plotted as percentages, (n=18, p < 0.0001 for each).
Figure 2. More Vials Frozen and Banked With FreSR™-S
hPSCs are cryopreserved in FreSR™-S at lower cell density compared to traditional methods. Graph indicates the number of vials cryopreserved for each well of a 6 well plate that is harvested.
Note: 1 vial is typically thawed and seeded directly into 1 well of a six well plate. Cells should be cultured as aggregates after thawing.
Figure 3. hPSCs Frozen and Thawed as Single Cells with FreSR™-S Display a Normal Karyotype
Karyograms of (A-B) WLS-4D1 hiPS cells and (C-D) H1 hES cells that were frozen and thawed as single cells using FreSR™-S. (A, C) Thawed cells were seeded into culture containing TeSR™-E8™ medium and 10 µM Y-27632 and maintained as aggregates for five passages. (B,D) hPSCs were also subjected to a second freeze-thaw cycle as single cells with FreSR™-S and cultured for five passages as aggregates prior to collection of karyotype data.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
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PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.