RosetteSep™ Human CD4+CD127low T Cell Enrichment Cocktail

Immunodensity negative selection cell isolation cocktail

RosetteSep™ Human CD4+CD127low T Cell Enrichment Cocktail

Immunodensity negative selection cell isolation cocktail

From: 1,154 USD
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Immunodensity negative selection cell isolation cocktail
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Product Advantages


  • Fast and easy-to-use

  • Requires no special equipment or training

  • Isolated cells are untouched

  • Can be combined with SepMate™ for consistent, high-throughput sample processing


What's Included

  • RosetteSep™ Human CD4+CD127low T Cell Enrichment Cocktail (Catalog #15361)
    • RosetteSep™ Human CD4+CD127low T Cell Enrichment Cocktail, 5 x 2 mL
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.

Overview

Easily isolate highly enriched CD4+CD127low T cells using the RosetteSep™ Human CD4+CD127low T Cell Enrichment Cocktail. CD127, the α-chain of the IL-7 receptor, plays a critical role in distinguishing human regulatory T cells (Tregs) from activated T cells. While it is broadly expressed on most mature T cells, where it supports proliferation and differentiation, regulatory T cells show little to no CD127 expression. This inverse correlation with FoxP3 makes CD127 an important marker to accurately identify and characterize T cell populations.

This RosetteSep™ Human CD4+CD127low T Cell Enrichment Cocktail is designed to isolate CD4+CD127low T cells directly from whole blood by negative selection. Unwanted cells are targeted for removal with tetrameric antibody complexes recognizing non-CD4+CD127low T cells and glycophorin A on red blood cells (RBCs). When centrifuged over a buoyant density medium such as Lymphoprep™, RosetteSep™ DM-L Density Medium, or RosetteSep™ DM-M Density Medium, the unwanted cells pellet along with the RBCs. The purified CD4+CD127low T cells are never labeled with antibodies and are easily collected as a highly enriched population at the interface between the plasma and the buoyant density medium. Isolated cells are immediately available for downstream applications such as flow cytometry, cell culture, or DNA/RNA extraction.
Cell Type
T Cells
Species
Human
Sample Source
Buffy Coat, Whole Blood
Selection Method
Negative
Application
Cell Isolation
Brand
RosetteSep
Area of Interest
Immunology

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
15361
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.