RoboSep™-C Human CD8+ T Cell Isolation Kit
Immunomagnetic negative selection kit
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In-lab demonstration
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RoboSep™-CAutomated closed-system immunomagnetic cell isolation instrument
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RoboSep™-C PBS/EDTA BufferCell separation buffer
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RoboSep™-C Tubing SetFor automated cell isolation with RoboSep™-C
Overview
This kit targets non-CD8+ T cells for removal with antibodies recognizing specific cell surface markers. Using RoboSep™-C in a fully automated protocol, unwanted cells are labeled with antibodies and magnetic particles, and separated without columns using a RoboSep™-C magnet. The desired untouched cells are washed and concentrated by RoboSep™-C and transferred to a final product bag. Isolated cells are immediately available for downstream applications such as flow cytometry, culture, or cryopreservation.
Data Figures
Figure 1. Typical RoboSep™-C Human CD8+ T Cell Isolation Profile
Starting with a fresh human peripheral blood leukopak, the CD8+ T cell content (CD3+CD8+) of the isolated fraction is typically 86.8 ± 5.4% (mean ± SD). In the above example, the purities of the start and final isolated fractions are 16.5% and 93.3%, respectively.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (6)
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
