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RoboSep™-C Human CD8+ T Cell Isolation Kit

Immunomagnetic negative selection kit

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RoboSep™-C Human CD8+ T Cell Isolation Kit

Immunomagnetic negative selection kit

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Immunomagnetic negative selection kit
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Product Advantages


  • Fully automated large-scale isolation in a closed system

  • Up to 94% purity with high recovery

  • Untouched, viable cells

What's Included

  • RoboSep™-C Human CD8+ T Cell Isolation Cocktail, 10 mL
  • RoboSep™-C Dextran RapidSpheres™ 30106, 10 mL
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. RoboSep™-C
    RoboSep™-C

    Automated closed-system immunomagnetic cell isolation instrument

  2. RoboSep™-C PBS/EDTA Buffer
    RoboSep™-C PBS/EDTA Buffer

    Cell separation buffer

  3. RoboSep™-C Tubing Set
    RoboSep™-C Tubing Set

    For automated cell isolation with RoboSep™-C

Overview

The RoboSep™-C Human CD8+ T Cell Isolation Kit is designed to isolate untouched and highly purified CD8+ T cells from fresh human peripheral blood leukopaks using RoboSep™-C (Catalog #100-0185 and #100-0186) for automated immunomagnetic negative selection.

This kit targets non-CD8+ T cells for removal with antibodies recognizing specific cell surface markers. Using RoboSep™-C in a fully automated protocol, unwanted cells are labeled with antibodies and magnetic particles, and separated without columns using a RoboSep™-C magnet. The desired untouched cells are washed and concentrated by RoboSep™-C and transferred to a final product bag. Isolated cells are immediately available for downstream applications such as flow cytometry, culture, or cryopreservation.
Magnet Compatibility
• RoboSep™-C (Catalog #100-0185)
• RoboSep™-C (Catalog #100-0186)
Subtype
Cell Isolation Kits
Cell Type
T Cells, T Cells, CD8+
Species
Human
Sample Source
Leukapheresis
Selection Method
Negative
Application
Cell Isolation
Brand
RoboSep
Area of Interest
Drug Discovery and Toxicity Testing, Immunology, Cell Therapy Development

Data Figures

FACS Profile Results with RoboSep™-C Human CD8+ T Cell Isolation Kit

Figure 1. Typical RoboSep™-C Human CD8+ T Cell Isolation Profile

Starting with a fresh human peripheral blood leukopak, the CD8+ T cell content (CD3+CD8+) of the isolated fraction is typically 86.8 ± 5.4% (mean ± SD). In the above example, the purities of the start and final isolated fractions are 16.5% and 93.3%, respectively.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
RoboSep™-C Human CD8+ T Cell Isolation Kit
Catalog #
100-0202
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™-C Human CD8+ T Cell Isolation Kit
Catalog #
100-0202
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™-C Human CD8+ T Cell Isolation Kit
Catalog #
100-0202
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (6)

T Cell Reagents for Your Cellular Therapy Research
Brochure
T Cell Reagents for Your Cellular Therapy Research
Tools for Your Immunology Research
Brochure
Tools for Your Immunology Research
Genome Editing of Human Primary T Cells Using CRISPR-Cas9
Technical Bulletin
Genome Editing of Human Primary T Cells Using CRISPR-Cas9
Production of Chimeric Antigen Receptor T Cells
Wallchart
Production of Chimeric Antigen Receptor T Cells
CAR T Cell Manufacturing Workflow: Isolation, Activation and Expansion
0:59
Video
CAR T Cell Manufacturing Workflow: Isolation, Activation and Expansion
Quick and Easy Isolation of T Cells and Other Immune Cells from Large-Volume Samples
37:09
Webinar
Quick and Easy Isolation of T Cells and Other Immune Cells from Large-Volume Samples

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Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.

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