EasySep™ Mouse Pan-DC Enrichment Kit II

Immunomagnetic negative selection kit

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From: 834 USD

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Immunomagnetic negative selection kit
From: 834 USD

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Overview Related Products Scientific Resources Product Applications Data and Publications

Overview

The EasySep™ Mouse Pan-DC Enrichment Kit II is designed to isolate dendritic cells (DCs) from mouse splenocytes by immunomagnetic negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.

This product replaces the EasySep™ Mouse Pan-DC Enrichment Kit (Catalog #19763), for improved yield and even faster cell isolations.
Advantages:
⦁ Fast, easy-to-use, and column-free
⦁ Up to 65% purity
⦁ Isolated cells are untouched
⦁ Optimized for high yield
⦁ Facilitates rapid flow sorting of DC subsets
Components:
  • EasySep™ Mouse Pan-DC Enrichment Kit (Catalog #19863)
    • EasySep™ Mouse Pan-DC Enrichment Cocktail II, 1 mL
    • EasySep™ Streptavidin RapidSpheres™ 50001, 1 mL
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype:
Cell Isolation Kits
Cell Type:
Dendritic Cells
Species:
Mouse
Sample Source:
Spleen
Selection Method:
Negative
Application:
Cell Isolation
Brand:
EasySep; RoboSep
Area of Interest:
Immunology; Infectious Diseases

Scientific Resources

Product Documentation

Educational Materials

(5)
Brochure
EasySep™ Cell Separation Technology
Brochure
Cell Enrichment Prior to Cell Sorting
Wallchart
Antigen Processing and Presentation
Wallchart
Frequencies and Percentages of Mouse Immune Cell Types
Video
1:57
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area Workflow Stages for
Workflow Stages
Dendritic Cell Research
Cell Sourcing
Cell Isolation
Differentiation and Maturation
Cell Characterization

Data and Publications

Data

Flow Cytometry Profile Results with EasySep™ Mouse Pan-DC Enrichment Kit II

Figure 1. Typical EasySep™ Mouse Pan-DC Enrichment Profile

Starting with mouse splenocytes, the dendritic cell content (CD11c+lineage-) of the isolated fraction is typically 57.3 ± 5.5% (mean ± SD using the purple EasySep™ magnet). In the above example, the purities of the start and final enriched fractions are 3.9% and 62.4%, respectively.

Reduce sort time using EasySep™ Mouse Pan-DC Enrichment Kit II

Figure 2. Pre-enrichment with EasySep™ Reduces Sorting Time

Starting with mouse splenocytes, DCs and DC subsets were analyzed in parallel from unenriched or EasySep™-enriched samples by flow cytometry. Each sample was run for an equivalent amount of time with a consistent flow rate and the data was used to extrapolate the time required to sort 1 million pan-DCs, conventional DCs (cDCs), or plamacytoid DCs (pDCs). In all cases, the required FACS times were reduced with EasySep™-enriched samples compared to unenriched samples (n = 2, SD).

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.