EasySep™ Mouse Pan-DC Enrichment Kit II

Immunomagnetic negative selection kit
Catalog #
19863_C
Immunomagnetic negative selection kit
From: 851 USD
Required Products
  1. EasySep™ Magnet
    EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer

Overview

The EasySep™ Mouse Pan-DC Enrichment Kit II is designed to isolate dendritic cells (DCs) from mouse splenocytes by immunomagnetic negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.

This product replaces the EasySep™ Mouse Pan-DC Enrichment Kit (Catalog #19763), for improved yield and even faster cell isolations.
Advantages
⦁ Fast, easy-to-use, and column-free
⦁ Up to 65% purity
⦁ Isolated cells are untouched
⦁ Optimized for high yield
⦁ Facilitates rapid flow sorting of DC subsets
Components
  • EasySep™ Mouse Pan-DC Enrichment Kit (Catalog #19863)
    • EasySep™ Mouse Pan-DC Enrichment Cocktail II, 1 mL
    • EasySep™ Streptavidin RapidSpheres™ 50001, 1 mL
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
Dendritic Cells
Species
Mouse
Sample Source
Spleen
Selection Method
Negative
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Immunology, Infectious Diseases

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
EasySep™ Mouse Pan-DC Enrichment Cocktail II
Catalog #
19863
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
RoboSep™ Mouse Pan-DC Enrichment Kit II
Catalog #
19863RF
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
EasySep™ Mouse Pan-DC Enrichment Cocktail II
Catalog #
19863
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
RoboSep™ Mouse Pan-DC Enrichment Kit II
Catalog #
19863RF
Lot #
All
Language
English

Educational Materials (5)

Brochure
EasySep™ Cell Separation Technology
Brochure
Cell Enrichment Prior to Cell Sorting
Wallchart
Antigen Processing and Presentation
Wallchart
Frequencies and Percentages of Mouse Immune Cell Types
Video
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Flow Cytometry Profile Results with EasySep™ Mouse Pan-DC Enrichment Kit II

Figure 1. Typical EasySep™ Mouse Pan-DC Enrichment Profile

Starting with mouse splenocytes, the dendritic cell content (CD11c+lineage-) of the isolated fraction is typically 57.3 ± 5.5% (mean ± SD using the purple EasySep™ magnet). In the above example, the purities of the start and final enriched fractions are 3.9% and 62.4%, respectively.

Reduce sort time using EasySep™ Mouse Pan-DC Enrichment Kit II

Figure 2. Pre-enrichment with EasySep™ Reduces Sorting Time

Starting with mouse splenocytes, DCs and DC subsets were analyzed in parallel from unenriched or EasySep™-enriched samples by flow cytometry. Each sample was run for an equivalent amount of time with a consistent flow rate and the data was used to extrapolate the time required to sort 1 million pan-DCs, conventional DCs (cDCs), or plamacytoid DCs (pDCs). In all cases, the required FACS times were reduced with EasySep™-enriched samples compared to unenriched samples (n = 2, SD).

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