EasySep™ Mouse CD90.1 Positive Selection Kit

Immunomagnetic positive selection cell isolation kit
Catalog #
18958_C
Immunomagnetic positive selection cell isolation kit
From: 712 USD
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Required Products
  1. EasyEights™ EasySep™ Magnet
    EasyEights™ EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer

Overview

The EasySep™ Mouse CD90.1 Positive Selection Kit is designed to isolate CD90.1+ (Thy1.1+) cells from single suspensions of splenocytes, lymph nodes and peripheral blood by positive selection. Desired cells are targeted with antibody complexes recognizing CD90.1 and dextran-coated magnetic particles. Labelled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.
Advantages
• Fast, and easy-to-use
• Up to 97% purity
• No columns required
• Isolated cells are not fluorochrome-labeled
Components
  • EasySep™ Mouse CD90.1 Positive Selection Kit (Catalog #18958)
    • EasySep™ Mouse CD90.1 Positive Selection Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™ 50100, 2 x 1 mL
  • RoboSep™ Mouse CD90.1 Positive Selection Kit (Catalog #18958RF)
    • EasySep™ Mouse CD90.1 Positive Selection Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™ 50100, 2 x 1 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyPlate™ EasySep™ Magnet (Catalog #18102)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™- S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
T Cells
Species
Mouse
Sample Source
Other, Spleen
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Immunology

Related Products

Labeling Antibodies

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
EasySep™ Mouse CD90.1 Positive Selection Kit
Catalog #
18958
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
RoboSep™ Mouse CD90.1 Positive Selection Kit
Catalog #
18958RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Mouse CD90.1 Positive Selection Kit
Catalog #
18958
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Mouse CD90.1 Positive Selection Kit
Catalog #
18958
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ Mouse CD90.1 Positive Selection Kit
Catalog #
18958RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ Mouse CD90.1 Positive Selection Kit
Catalog #
18958RF
Lot #
All
Language
English

Educational Materials(10)

Brochure
Tools For Your Immunology Research
Brochure
EasySep™ Cell Separation Technology
Brochure
Mouse Cell Isolation
Wallchart
Frequencies and Percentages of Mouse Immune Cell Types
Wallchart
Human Immune Cytokines
Video
0:57
Simultaneous Cell Isolation from Multiple Samples Using the EasyEights™ EasySep™ Magnet
Video
0:01
EasySep™ Mouse Cell Isolation Kits: How Much Time Will You Save?
Video
1:57
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
Video
1:13
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
Webinar
34:58
Using Stable Isotope Tracing to Study CD8+ Immune Cell Dynamics In Vivo

Frequently Asked Question

Can EasySep™ be used for either positive or negative selection?

Yes. The EasySep™ kits use either a negative selection approach by targeting and removing unwanted cells or a positive selection approach targeting desired cells. Depletion kits are also available for the removal of cells with a specific undesired marker (e.g. GlyA).

How does the separation work?

Magnetic particles are crosslinked to cells using Tetrameric Antibody Complexes (TAC). When placed in the EasySep™ Magnet, labeled cells migrate to the wall of the tube. The unlabeled cells are then poured off into a separate fraction.

Which columns do I use?

The EasySep™ procedure is column-free. That's right - no columns!

How can I analyze the purity of my enriched sample?

The Product Information Sheet provided with each EasySep™ kit contains detailed staining information.

Can EasySep™ separations be automated?

Yes. RoboSep™, the fully automated cell separator, automates all EasySep™ labeling and cell separation steps.

Can EasySep™ be used to isolate rare cells?

Yes. We recommend a cell concentration of 2x108 cells/mL and a minimum working volume of 100 µL. Samples containing 2x107 cells or fewer should be suspended in 100 µL of buffer.

Are the EasySep™ magnetic particles FACS-compatible?

Yes, the EasySep™ particles are flow cytometry-compatible, as they are very uniform in size and about 5000X smaller than other commercially available magnetic beads used with column-free systems.

Can the EasySep™ magnetic particles be removed after enrichment?

No, but due to the small size of these particles, they will not interfere with downstream applications.

Can I alter the separation time in the magnet?

Yes; however, this may impact the kit's performance. The provided EasySep™ protocols have already been optimized to balance purity, recovery and time spent on the isolation.

For positive selection, can I perform more than 3 separations to increase purity?

Yes, the purity of targeted cells will increase with additional rounds of separations; however, cell recovery will decrease.

How does the binding of the EasySep™ magnetic particle affect the cells? is the function of positively selected cells altered by the bound particles?

Hundreds of publications have used cells selected with EasySep™ positive selection kits for functional studies. Our in-house experiments also confirm that selected cells are not functionally altered by the EasySep™ magnetic particles.

If particle binding is a key concern, we offer two options for negative selection. The EasySep™ negative selection kits can isolate untouched cells with comparable purities, while RosetteSep™ can isolate untouched cells directly from whole blood without using particles or magnets.
Read More

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

FACS Histogram Results with EasySep™ Mouse CD90.1 Positive Selection Kit

Figure 1. FACS Histogram Results with EasySep™ Mouse CD90.1 Positive Selection Kit

Starting with mouse splenocytes, the CD90.1+ (Thy1.1+) cell content of the isolated fraction is typically 93.5 ± 3.9% (mean ± SD; using the purple EasySep™ Magnet).

FACS Histogram Results with EasySep™ Mouse CD90.1 Positive Selection Kit on Adoptive Lymphocyte Transfer Samples

Figure 2. FACS Histogram Results with EasySep™ Mouse CD90.1 Positive Selection Kit on Adoptive Lymphocyte Transfer Samples

Starting with mouse splenocytes from adoptive lymphocyte transfer samples, the CD90.1+ (Thy1.1+) cell content of the isolated fraction is typically 90.8 ± 1.5% (mean ± SD; using the purple EasySep™ Magnet).

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