EasySep™ Human TCR Alpha/Beta Depletion Kit

Immunomagnetic depletion kit
EasySep™ Human TCR Alpha/Beta Depletion Kit

Immunomagnetic depletion of human TCRa/b+ cells

Catalog # 17847
670 USD
Required Products
  1. EasySep™ Magnet
    EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer

Overview

The EasySep™ Human TCR Alpha/Beta Depletion Kit is designed to deplete human T cell receptor alpha/beta+ (TCRab+) cells from leukapheresis samples or expanded TCRa/b knockout cell cultures. TCRab+ cells are targeted with antibody complexes and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Labeled cells remain in the tube while unlabeled cells are poured off.
Advantages
⦁ Fast, easy-to-use, and column-free procedure
⦁ Untouched, viable cells
Components
  • EasySep™ Human TCR Alpha/Beta Depletion Kit (Catalog #17847)
    • EasySep™ Human TCR Alpha/Beta Depletion Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™, 2 x 1 mL
    Magnet Compatibility
    • EasySep™ Magnet (Catalog #18000)
    • “The Big Easy” EasySep™ Magnet (Catalog #18001)
    • Easy 50 EasySep™ Magnet (Catalog #18002)
    Subtype
    Cell Isolation Kits
    Cell Type
    T Cells
    Species
    Human
    Sample Source
    Leukapheresis, Other
    Selection Method
    Depletion
    Application
    Cell Isolation
    Brand
    EasySep
    Area of Interest
    Cell Therapy, Immunology

    Scientific Resources

    Product Documentation

    Document Type Product Name Catalog # Lot # Language
    Document Type
    Product Information Sheet
    Product Name
    EasySep™ Human TCR Alpha/Beta Depletion Kit
    Catalog #
    17847
    Lot #
    All
    Language
    English
    Document Type
    Safety Data Sheet 1
    Product Name
    EasySep™ Human TCR Alpha/Beta Depletion Kit
    Catalog #
    17847
    Lot #
    All
    Language
    English
    Document Type
    Safety Data Sheet 2
    Product Name
    EasySep™ Human TCR Alpha/Beta Depletion Kit
    Catalog #
    17847
    Lot #
    All
    Language
    English

    Educational Materials(7)

    Brochure
    EasySep™ Cell Separation Technology
    Brochure
    ArciTect™ CRISPR-Cas9 Genome Editing System
    Technical Bulletin
    Genome Editing of Human Primary T Cells Using CRISPR-Cas9
    Wallchart
    Production of Chimeric Antigen Receptor T Cells
    Video
    1:13
    Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
    Video
    1:57
    How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
    Scientific Poster
    Optimization of Human T Cell Activation and Expansion Protocols Improves Efficiency of Genetic Modification and Overall Cell Yield

    Product Applications

    This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

    Data and Publications

    Data

    The content of TCRab+ cells before and after the cell isolation procedure are 19.1% and 0.1%, respectively.

    Figure 1. EasySep™ Human TCR Alpha/Beta Depletion Kit

    In the above example, the frequencies of CD3+TCRαß+ cells in the starting and depleted fractions are 19.1% and 0.1%, respectively.

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