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EasySep™ HLA Chimerism Buffy Coat CD56 Positive Selection Kit

Immunomagnetic positive selection cell isolation kit

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From: 581 USD

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Immunomagnetic positive selection cell isolation kit
From: 581 USD

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Overview

The EasySep™ HLA Chimerism Buffy Coat CD56 Positive Selection Kit II is designed to isolate CD56+ cells from freshly prepared buffy coat by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD56 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD56 antigen is expressed on NK cells and NKT cells.

This product replaces the EasySep™ Human Buffy Coat CD56 Positive Selection Kit (Catalog #18085) and EasySep™ HLA Buffy Coat CD56 Positive Selection Kit (Catalog #18085HLA), for even faster cell isolations.
Advantages:
• Fast and easy-to-use
• Up to 99% purity
• No columns required
Components:
    • EasySep™ HLA Chimerism Buffy Coat CD56 Positive Selection Kit (Catalog #17875)
        • EasySep™ HLA Chimerism Buffy Coat CD56 Positive Selection Cocktail, 2 x 0.75 mL
        • EasySep™ Dextran RapidSpheres, 2 x 1 mL
        • EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
    • RoboSep™ HLA Chimerism Buffy Coat CD56 Positive Selection Kit with Filter Tips (Catalog #17875RF)
        • EasySep™ HLA Chimerism  Buffy Coat CD56 Positive Selection Cocktail, 2 x 0.75 mL
        • EasySep™ Dextran RapidSpheres, 2 x 1 mL
        • EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
        • RoboSep™ Buffer (Catalog #20104) x 2
RoboSep™ Filter Tips (Catalog #20125) x 2
Magnet Compatibility:
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• RoboSep™-S (Catalog #21000)
• EasyEights™- EasySep™ Magnet (Catalog #18103)
Subtype:
Cell Isolation Kits
Cell Type:
NK Cells
Species:
Human
Sample Source:
Buffy Coat
Selection Method:
Positive
Brand:
EasySep
Area of Interest:
HLA; Immunology; Chimerism

Scientific Resources

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Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Starting with freshly prepared buffy coat, the CD56+ cell content of the isolated fraction typically ranges from 95.8 - 99.5% (gated on CD45+ using “The Big Easy” EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 8.5% and 98.4%, respectively. NOTE: RBCs were removed from the start sample by lysis prior to flow cytometry.

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.