EasySep™ HLA Chimerism Buffy Coat CD14 Positive Selection Kit

Immunomagnetic positive selection cell isolation kit
Catalog #
17878_C
Immunomagnetic positive selection cell isolation kit
From: 623 USD
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Required Products
  1. "The Big Easy" EasySep™ Magnet
    "The Big Easy" EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

Overview

The EasySep™ HLA Chimerism Buffy Coat CD14 Positive Selection Kit is designed to isolate CD14+ cells from freshly prepared buffy coat by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD14 and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD14 antigen is strongly expressed on monocytes and is weakly expressed on granulocytes. It is also expressed on most tissue macrophages.

This product replaces the EasySep™ Human Buffy Coat CD14 Positive Selection Kit (Catalog #18088) for even faster cell isolations.
Advantages
• Fast and easy-to-use
• Up to 99% purity
• No columns required
Components
  • EasySep™ HLA Chimerism Buffy Coat CD14 Positive Selection Kit (Catalog #17878)
    • EasySep™ HLA Chimerism Buffy Coat CD14 Positive Selection Cocktail, 2 x 0.75 mL
    • EasySep™ Dextran RapidSpheres 50100, 2 x 1 mL
    • EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
  • RoboSep™ HLA Chimerism Buffy Coat CD14 Positive Selection Kit with Filter Tips (Catalog #17878RF)
    • EasySep™ HLA Chimerism Buffy Coat CD14 Positive Selection Cocktail, 2 x 0.75 mL
    • EasySep™ Dextran RapidSpheres, 50100, 2 x 1 mL
    • EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
    • RoboSep™ Buffer (Catalog #20104) x 2
    • RoboSep™ Filter Tips (Catalog #20125) x 2
Magnet Compatibility
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
 
Subtype
Cell Isolation Kits
Cell Type
Monocytes
Species
Human
Sample Source
Buffy Coat
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Chimerism, Immunology

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet - Buffy Coat
Product Name
EasySep™ HLA Chimerism Buffy Coat CD14 Positive Selection Kit
Catalog #
17878
Lot #
All
Language
English
Document Type
Product Information Sheet - Leukoreduction System Chamber/Cone
Product Name
EasySep™ HLA Chimerism Buffy Coat CD14 Positive Selection Kit
Catalog #
17878
Lot #
All
Language
English
Document Type
Product Information Sheet - Buffy Coat
Product Name
RoboSep™ HLA Chimerism Buffy Coat CD14 Positive Selection Kit
Catalog #
17878RF
Lot #
All
Language
English
Document Type
Product Information Sheet - Leukoreduction System Chamber/Cone
Product Name
RoboSep™ HLA Chimerism Buffy Coat CD14 Positive Selection Kit
Catalog #
17878RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ HLA Chimerism Buffy Coat CD14 Positive Selection Kit
Catalog #
17878
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ HLA Chimerism Buffy Coat CD14 Positive Selection Kit
Catalog #
17878
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
EasySep™ HLA Chimerism Buffy Coat CD14 Positive Selection Kit
Catalog #
17878
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
EasySep™ HLA Chimerism Buffy Coat CD14 Positive Selection Kit
Catalog #
17878
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ HLA Chimerism Buffy Coat CD14 Positive Selection Kit
Catalog #
17878RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ HLA Chimerism Buffy Coat CD14 Positive Selection Kit
Catalog #
17878RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ HLA Chimerism Buffy Coat CD14 Positive Selection Kit
Catalog #
17878RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
RoboSep™ HLA Chimerism Buffy Coat CD14 Positive Selection Kit
Catalog #
17878RF
Lot #
All
Language
English

Educational Materials (12)

Brochure
EasySep™ Cell Separation Technology
Brochure
HLA Antibodies for Purity Assessment
Brochure
Cell Separation Products for HLA Analysis
Technical Bulletin
Purity Assessment by Flow Cytometry for Lineage-Specific Chimerism Analysis
Technical Bulletin
Fully Automated, High Purity Cell Isolation for Chimerism Labs
Wallchart
Frequencies of Cell Types in Human Peripheral Blood
Wallchart
Human Immune Cytokines
Video
How to Recover Cells from a Leukocyte Reduction System (LRS) Cone
4:55
How to Recover Cells from a Leukocyte Reduction System (LRS) Cone
Video
Automate Cell Isolation with the RoboSep™-S Cell Separation Instrument
1:41
Automate Cell Isolation with the RoboSep™-S Cell Separation Instrument
Video
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
Webinar
Fully Automated Cell Separation for Chimerism Analysis with RoboSep™
56:02
Fully Automated Cell Separation for Chimerism Analysis with RoboSep™
Scientific Poster
Isolation of Very Pure Lymphoid (CD3+, CD19+ or CD56+) and Myeloid (CD15+ or CD33/CD66b+) Cell Subsets in as Little as 15 Minutes for Use in Lineage-Specific Chimerism Analysis

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Starting with buffy coat, the CD14+ cell content of the isolated fraction is typically 93.0 ± 6.4% (mean ± SD using “The Big Easy” EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 8.9% and 98.4%, respectively.

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