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EasySep™ HLA Chimerism Buffy Coat CD14 Positive Selection Kit

Immunomagnetic positive selection cell isolation kit

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From: 593 USD

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Immunomagnetic positive selection cell isolation kit
From: 593 USD

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Overview

The EasySep™ HLA Chimerism Buffy Coat CD14 Positive Selection Kit is designed to isolate CD14+ cells from freshly prepared buffy coat by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD14 and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD14 antigen is strongly expressed on monocytes and is weakly expressed on granulocytes. It is also expressed on most tissue macrophages.

This product replaces the EasySep™ Human Buffy Coat CD14 Positive Selection Kit (Catalog #18088) for even faster cell isolations.
Advantages:
• Fast and easy-to-use
• Up to 99% purity
• No columns required
Components:
EasySep™ HLA Chimerism Buffy Coat CD14 Positive Selection Kit (Catalog #17878)
EasySep™ HLA Chimerism Buffy Coat CD14 Positive Selection Cocktail, 2 x 0.75 mL
EasySep™ Dextran RapidSpheres 50100, 2 x 1 mL
EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
RoboSep™ HLA Chimerism Buffy Coat CD14 Positive Selection Kit with Filter Tips (Catalog #17878RF)
EasySep™ HLA Chimerism Buffy Coat CD14 Positive Selection Cocktail, 2 x 0.75 mL
EasySep™ Dextran RapidSpheres, 50100, 2 x 1 mL
EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
RoboSep™ Buffer (Catalog #20104) x 2
RoboSep™ Filter Tips (Catalog #20125) x 2
Magnet Compatibility:
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
 
Subtype:
Cell Isolation Kits
Cell Type:
Monocytes
Species:
Human
Sample Source:
Buffy Coat; Whole Blood
Selection Method:
Positive
Application:
Cell Isolation
Brand:
EasySep; RoboSep
Area of Interest:
Chimerism; Immunology

Scientific Resources

Educational Materials

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Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Starting with buffy coat, the CD14+ cell content of the isolated fraction is typically 93.0 ± 6.4% (mean ± SD using “The Big Easy” EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 8.9% and 98.4%, respectively.

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.