MSC Characterization Antibody Panel

Multi-color flow cytometry kit for characterization of human mesenchymal stromal cells

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MSC Characterization Antibody Panel

Multi-color flow cytometry kit for characterization of human
mesenchymal stromal cells

1 Kit
Catalog #100-0354
789 USD

Overview

The MSC Characterization Antibody Panel is designed for the phenotypic analysis of human mesenchymal stromal cells (MSCs). This kit contains three fluorochrome-conjugated antibodies for the identification of human MSCs, and a fluorochrome-conjugated antibody for the identification of non-MSCs. When measured by flow cytometry, ≥ 95% of the MSC population must express CD73, CD90, and CD105, and these cells must lack expression (≤ 2% positive) of CD45. These reagents provide single-step labeling for the verification of human MSCs.The MSC Characterization Antibody Panel can be used to identify human MSCs derived from various tissues such as bone marrow MSCs, pluripotent stem cell-derived mesenchymal progenitor cells, adipose-derived MSCs, dental pulp-derived MSCs, and umbilical cord-derived MSCs.
Subtype:
Primary Antibodies
Cell Type:
Mesenchymal Stromal Cells
Application:
Flow Cytometry
Area of Interest:
Endothelial Cell Biology; Stem Cell Biology

Scientific Resources

Product Documentation

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Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Flow cytometry dot plots comparing the difference in expression of CD45, CD73, CD90, and CD105 in human bone marrow-derived mesenchymal stromal cells (MSCs) labeled with the hMSC CD45/CD73/CD90/CD105 Antibody Panel and cultured either in MesenCult™-ACF Plus Medium or in serum-containing medium.

Figure 1. Flow Cytometry Analysis of Human Bone Marrow-Derived Mesenchymal Stromal Cells Labeled Using the hMSC CD45/CD73/CD90/CD105 Antibody Panel

(A) When cultured in MesenCult™-ACF Plus Medium, the labeled human bone marrow (BM)-derived mesenchymal stromal cells (MSCs), demonstrated positive expression of CD73, CD105, and CD90 as well as negative expression of CD45 at P0. (B) When cultured in serum-containing medium, the BM-derived MSCs exhibit reduced CD73, CD105, and CD90 expression as well as increased CD45 expression at P0 due to contaminating CD45+ cells.

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.