Mouse Pancreatic Organoids

Cryopreserved mouse pancreatic exocrine organoids for establishment of organoid cultures
Mouse Pancreatic Organoids

Cryopreserved mouse pancreatic exocrine organoids for establishment of organoid cultures

200 Organoids
Catalog # 70933
795 USD
Required Products
  1. DMEM/F-12 with 15 mM HEPES
    DMEM/F-12 with 15 mM HEPES

    Dulbecco's Modified Eagle's Medium/Nutrient Ham's Mixture F-12 (DMEM/F-12) with 15 mM HEPES buffer

  2. PancreaCult™ Organoid Growth Medium (Mouse)
    PancreaCult™ Organoid Growth Medium (Mouse)

    Cell culture medium for establishment and maintenance of mouse pancreatic exocrine organoids

Overview

Cryopreserved Mouse Pancreatic Organoids provide a convenient way to establish or standardize pancreatic organoid cultures in the laboratory. Each vial contains fragments of mouse pancreatic exocrine organoids sufficient to establish two wells of organoid cultures. The organoids are derived from the pancreas of C57BL/6 mice, cultured in PancreaCult™ Organoid Growth Medium (Mouse; Catalog #06040) and cryopreserved in CryoStor® CS10 (Catalog #07930). Using cryopreserved Mouse Pancreatic Organoids enables establishment of pancreatic organoid cultures without the need to isolate pancreatic ducts from primary tissue, eliminating the need for access to fresh mouse tissue. The organoids can be passaged and expanded using PancreaCult™ Organoid Growth Medium (Mouse) and cryopreserved in CryoStor® CS10. Mouse pancreatic exocrine organoid cultures can be used for research in a variety of fields, including epithelial cell biology, cancer, and metabolism.
Advantages
• Cryopreserved cells enable establishment of organoid cultures without isolation of ducts from primary mouse pancreatic tissue
• Convenient, in vitro model system of the exocrine compartment of the pancreatic epithelium
Contains
• Frozen mouse pancreatic organoid segments
• CryoStor® CS10 (Catalog #07930)
Cell Type
Pancreatic Cells
Species
Mouse

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Mouse Pancreatic Organoids
Catalog #
70933
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Mouse Pancreatic Organoids
Catalog #
70933
Lot #
All
Language
English

Educational Materials (10)

Brochure
2019-2020 Cell Culture Training Catalog
Technical Bulletin
Mouse Pancreatic Exocrine Organoid Culture: Supplementary Protocols
Video
Growth of Mouse Pancreatic Organoids in PancreaCult™ Culture Medium: Under a Microscope
1:07
Growth of Mouse Pancreatic Organoids in PancreaCult™ Culture Medium: Under a Microscope
Video
Growth of Liver Organoids in HepatiCult™ Culture Medium: Under a Microscope
1:18
Growth of Liver Organoids in HepatiCult™ Culture Medium: Under a Microscope
Webinar
Challenges in Translating iPSC Technology
41:47
Challenges in Translating iPSC Technology
Webinar
GI Tract Organoids: Using Advanced Tissue Models to Interrogate Absorption and Regulation
24:56
GI Tract Organoids: Using Advanced Tissue Models to Interrogate Absorption and Regulation
Scientific Poster
Culture and Differentiation of Mouse Hepatic Organoids Using Serum-free HepatiCult™ Organoid Growth Medium
Scientific Poster
Modelling Pancreatic Cancer Through Pancreatic Exocrine Organoids Using PancreaCult™ Serum-free Medium
Scientific Poster
Highly Efficient Differentiation of Human Pluripotent Stem Cells into Long-term Expandable “Mini-gut” Organoids
Interview
Luigi Aloia, PhD

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages
Workflow Stages for Organoids

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