Mouse Intestinal Organoids

Cryopreserved mouse intestinal epithelial organoids for establishment of organoid cultures
Mouse Intestinal Organoids

Cryopreserved mouse intestinal epithelial organoids for establishment of organoid cultures

200 Organoids
Catalog # 70931
757 USD
Required Products
  1. IntestiCult™ Organoid Growth Medium (Mouse)
    IntestiCult™ Organoid Growth Medium (Mouse)

    Cell culture medium for establishment and maintenance of mouse intestinal organoids

  2. DMEM/F-12 with 15 mM HEPES
    DMEM/F-12 with 15 mM HEPES

    Dulbecco's Modified Eagle's Medium/Nutrient Ham's Mixture F-12 (DMEM/F-12) with 15 mM HEPES buffer

Overview

Cryopreserved Mouse Intestinal Organoids provide a convenient way to establish or standardize intestinal organoid cultures in your laboratory. Each vial contains 200 mouse intestinal organoids derived from the small intestine of C57BL/6 mice that were cultured in IntestiCult™ Organoid Growth Medium (Mouse) and cryopreserved in CryoStor® CS10. Using cryopreserved Mouse Intestinal Organoids enables establishment of intestinal organoid cultures without the need to isolate intestinal crypts from primary tissue, eliminating the need for access to fresh mouse tissue and making it easy to standardize experimental starting materials. The organoids can be passaged and expanded using IntestiCult™ Organoid Growth Medium (Mouse) and refrozen in CryoStor® CS10. Mouse intestinal organoid cultures can be used for research in a variety of fields, including epithelial cell biology, cancer, cystic fibrosis, microbiomics, intestinal immunology, and bacterial or viral pathogenesis.
Advantages
• Cryopreserved cells enable establishment of organoid cultures without isolation of crypts from primary mouse intestinal tissue
• Convenient, in vitro system that recapitulates the identity and organization of the adult intestinal epithelium, including intra- and intercellular signaling, self-propagating stem cell niche and functional transport into and out of the lumen
Contains
• Frozen mouse intestinal organoid segments
• CryoStor® CS10 (Catalog #07930)
Subtype
Frozen
Cell Type
Intestinal Cells
Species
Mouse
Cell and Tissue Source
Other
Donor Status
Normal

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Mouse Intestinal Organoids
Catalog #
70931
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Mouse Intestinal Organoids
Catalog #
70931
Lot #
All
Language
English

Educational Materials(10)

Brochure
2019-2020 Cell Culture Training Catalog
Video
1:18
Growth of Liver Organoids in HepatiCult™ Culture Medium: Under a Microscope
Video
1:09
How to Count Intestinal Crypts for Mouse Organoid Cultures
Video
1:07
Growth of Mouse Pancreatic Organoids in PancreaCult™ Culture Medium: Under a Microscope
Webinar
41:47
Challenges in Translating iPSC Technology
Webinar
24:56
GI Tract Organoids: Using Advanced Tissue Models to Interrogate Absorption and Regulation
Scientific Poster
Efficient Establishment and Long-term Maintenance of 3-dimensional Human Small Intestinal and Colonic Organoids Using a Novel IntestiCult™ Organoid Growth Medium (Human)
Scientific Poster
Highly Efficient Differentiation of Human Pluripotent Stem Cells into Long-term Expandable “Mini-gut” Organoids
Interview
Luigi Aloia, PhD
Interview
Heather McCauley, PhD

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

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