Mouse Intestinal Organoids
Cryopreserved mouse intestinal epithelial organoids for establishment of organoid cultures
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Label for Mouse Intestinal Organoids -
Mouse Intestinal Organoids
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Required Products
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IntestiCult™ Organoid Growth Medium (Mouse)
Cell culture medium for establishment and maintenance of mouse intestinal organoids -
Dulbecco's Modified Eagle's Medium/Nutrient Ham's Mixture F-12 (DMEM/F-12) with 15 mM HEPES buffer
Overview
Cryopreserved Mouse Intestinal Organoids provide a convenient way to establish or standardize intestinal organoid cultures in your laboratory. Each vial contains 200 mouse intestinal organoids derived from the small intestine of C57BL/6 mice that were cultured in IntestiCult™ Organoid Growth Medium (Mouse) and cryopreserved in CryoStor® CS10. Using cryopreserved Mouse Intestinal Organoids enables establishment of intestinal organoid cultures without the need to isolate intestinal crypts from primary tissue, eliminating the need for access to fresh mouse tissue and making it easy to standardize experimental starting materials. The organoids can be passaged and expanded using IntestiCult™ Organoid Growth Medium (Mouse) and refrozen in CryoStor® CS10. Mouse intestinal organoid cultures can be used for research in a variety of fields, including epithelial cell biology, cancer, cystic fibrosis, microbiomics, intestinal immunology, and bacterial or viral pathogenesis.
Advantages:
• Cryopreserved cells enable establishment of organoid cultures without isolation of crypts from primary mouse intestinal tissue
• Convenient, in vitro system that recapitulates the identity and organization of the adult intestinal epithelium, including intra- and intercellular signaling, self-propagating stem cell niche and functional transport into and out of the lumen
• Convenient, in vitro system that recapitulates the identity and organization of the adult intestinal epithelium, including intra- and intercellular signaling, self-propagating stem cell niche and functional transport into and out of the lumen
Contains:
• Frozen mouse intestinal organoid segments
• CryoStor® CS10 (Catalog #07930)
• CryoStor® CS10 (Catalog #07930)
Subtype:
Frozen
Cell Type:
Intestinal Cells
Species:
Mouse
Cell and Tissue Source:
Other
Donor Status:
Normal
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Animal component-free, defined cryopreservation medium with 10% DMSO -
Gentle Cell Dissociation Reagent
Enzyme-free cell dissociation reagent -
Online and in-person training courses to support the culture of intestinal organoids -
STEMdiff™ Definitive Endoderm Kit
Defined animal component-free medium for the differentiation of human ES and iPS cells to definitive endoderm
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This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
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This product was developed under a license to intellectual property owned by Hubrecht Organoid Technology (HUB). This product is sold for research use only. Purchase of this product does not include the right to use this product for growing organoids for drug screening aiming for commercial gain or for other commercial purposes. Purchasers wishing to use the product for purposes other than research use should contact HUB to obtain a further license (an “Organoid-Growth License”). Purchasers may apply for an Organoid-Growth License from HUB and such license will not be unreasonably withheld by HUB.
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.