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IntestiCult™ OGM Human Basal Medium

Cell culture medium for establishment and maintenance of human colorectal cancer organoids

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IntestiCult™ OGM Human Basal Medium

Cell culture medium for establishment and maintenance of human colorectal cancer organoids

From: 493 USD
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Cell culture medium for establishment and maintenance of human colorectal cancer organoids
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Product Advantages


  • Recapitulate tumor characteristics and heterogeneity with a physiologically relevant in vitro system

  • Predict patient response with highly representative tumoroids

  • Obtain consistent results with a complete medium formulation

  • Streamline your workflow with an easy-to-use format and optimized protocols

Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. Gentle Cell Dissociation Reagent
    Gentle Cell Dissociation Reagent

    cGMP, enzyme-free cell dissociation reagent

  2. 96-well Organoid Culture Plate
    Organoid Culture Plates

    Cell culture plates for easy and reproducible generation of organoids

  3. DMEM/F-12 with 15 mM HEPES
    DMEM/F-12 with 15 mM HEPES

    Dulbecco's Modified Eagle's Medium/Nutrient Ham's Mixture F-12 (DMEM/F-12) with 15 mM HEPES buffer

  4. Y-27632 (Dihydrochloride)
    Y-27632 (Dihydrochloride)

    RHO/ROCK pathway inhibitor; Inhibits ROCK1 and ROCK2

Overview

Efficiently grow Wnt-independent colorectal tumor organoids using IntestiCult™ OGM Human Basal Medium.

Tumoroids have high predictive value as an in vitro system as they retain the characteristics of the tumor of origin. Use IntestiCult™ OGM Human Basal Medium to provide optimal culture conditions for tumor organoid growth and expansion while maintaining tumor heterogeneity. The Wnt-free formulation ensures that Wnt-dependent, normal, healthy intestinal cells do not contaminate the tumoroid culture.

Tumor organoids generated using IntestiCult™ OGM Human Basal Medium are suitable for a variety of applications, including modeling tumor development and screening drugs for efficacy and toxicity.

For growing healthy intestinal organoids, please use IntestiCult™ Organoid Growth Medium (Human) or IntestiCult™-SF Organoid Growth Medium (Human). For differentiating human intestinal organoids, refer to IntestiCult™ Organoid Differentiation Medium (Human).

Learn how to culture human intestinal organoids in our On-Demand Intestinal Course or browse our Frequently Asked Questions (FAQs) about the organoid workflows using IntestiCult™. Additionally, download our detailed e-book Proven Protocols for Intestinal Organoid Culture: Getting Started with IntestiCult™ for a curated collection of intestinal organoid protocols.
Cell Type
Cancer Cells and Cell Lines, Epithelial Cells, Intestinal Cells
Species
Human
Application
Organoid Culture
Brand
IntestiCult
Area of Interest
Cancer, Disease Modeling, Drug Discovery and Toxicity Testing, Epithelial Cell Biology, Organoids

More Information

More Information
Safety Statement

CA WARNING: This product can expose you to Progesterone which is known to the State of California to cause cancer. For more information go to www.P65Warnings.ca.gov

Data Figures

Figure 1. Growth of Cancer-Derived Organoids in IntestiCult™ OGM Human Basal Medium

Organoids were established from colorectal cancer biopsies in published medium1, then switched to IntestiCult™ OGMH (P0), as described below. Cancer-derived organoids demonstrated efficient growth both after establishment in IntestiCult™ OGM Human Basal Medium, as well as after passaging. Data used with permission from Hubrecht Organoid Technology.

Figure 2. IntestiCult™ OGM Human Basal Medium Enables Organoid Growth Across Different Patients

Organoids were established in published medium1 from colorectal tumor biopsy samples, then switched to IntestiCult™ OGM Human Basal Medium after passaging as described above. Organoids were passaged twice in IntestiCult™ OGMH Basal Medium and imaged at the end of the second passage (day 6 - 12). Data used with permission from Hubrecht Organoid Technology.

Confocal images of human colorectal cancer organoids stained with Ki67, EPCAM, and DAPI.

Figure 3. Colorectal Cancer Organoids Reflect Donor-Specific Colonic Features

Representative confocal images of human colorectal cancer organoids from four donors, cultured using IntestiCult™ OGM Human Basal Medium. Organoids were fixed and stained for Ki67 (green), EPCAM (red) and DAPI (Blue). Donor-specific differences in morphology were observed, including polarization and hollow lumens (A, B), or denser structures, lacking distinct epithelial polarization (C, D). Scale bars = 50 µm.

IC50 toxicity graphs demonstrating the response of colorectal cancer organoids to various cancer drugs.

Figure 4. Colorectal Cancer Organoids Cultured in IntestiCult™OGM Human Basal Medium Are Sensitive to Drug-Induced Toxicity in a Dose-Dependent Manner

Colorectal cancer organoids (CRCs) from 4 donors were treated with increasing concentrations of (A) 5-FU, (B) Flavopiridol, (C) Gefitinib, or (D) Irinotecan, and were compared to organoids from a healthy colon donor for 5 days. Cell viability was assessed on Day 7 using the CellTiter-Glo® Cell Viability Assay (Promega). Error bars = SEM.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
IntestiCult™ OGM Human Basal Medium
Catalog #
100-0190
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
IntestiCult™ OGM Human Basal Medium
Catalog #
100-0190
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages
Workflow Stages for Organoids
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Resources and Publications

Educational Materials (20)

Intestinal Organoids
Brochure
Intestinal Organoids
CRISPR-Cas9 Genome Editing of Human Intestinal Organoids
Protocol
CRISPR-Cas9 Genome Editing of Human Intestinal Organoids
Barrier Permeability Assay Using Human Intestinal Organoid-Derived Monolayers
Protocol
Barrier Permeability Assay Using Human Intestinal Organoid-Derived Monolayers
How to Generate Human Intestinal Organoid-Derived Monolayers Using IntestiCult™
Protocol
How to Generate Human Intestinal Organoid-Derived Monolayers Using IntestiCult™
How to Thaw Intestinal Organoids
Protocol
How to Thaw Intestinal Organoids
Toxicity Testing for Drug Development Using Human Intestinal Organoids and IntestiCult™
Protocol
Toxicity Testing for Drug Development Using Human Intestinal Organoids and IntestiCult™
Culturing Intestinal Cancer-Derived Organoids
Protocol
Culturing Intestinal Cancer-Derived Organoids
How to Isolate Human Colonic Crypts from Intestinal Biopsies
Protocol
How to Isolate Human Colonic Crypts from Intestinal Biopsies
STEMCELL Seminar: Functional Applications of Intestinal Organoids
21:26
Webinar
STEMCELL Seminar: Functional Applications of Intestinal Organoids
Patient-Derived Organoids for Drug Screening and Development
50:25
Webinar
Patient-Derived Organoids for Drug Screening and Development
Nature Research Round Table: Organoids as an Enabling Technology for Precision Cancer Medicine
17:20
Webinar
Nature Research Round Table: Organoids as an Enabling Technology for Precision Cancer Medicine
From Stem Cells to Organoids: Modeling the Gastrointestinal Tract
1:03:40
Webinar
From Stem Cells to Organoids: Modeling the Gastrointestinal Tract
Intestinal Organoids: Improved Tools and In Vitro Models for Drug Development
17:32
Webinar
Intestinal Organoids: Improved Tools and In Vitro Models for Drug Development
HUB & STEMCELL Organoids as Models of Infectious Disease Mini-Symposium
1:10:09
Webinar
HUB & STEMCELL Organoids as Models of Infectious Disease Mini-Symposium
Advances in GI Organoid Applications: Drug Discovery and Immunotherapy
47:49
Webinar
Advances in GI Organoid Applications: Drug Discovery and Immunotherapy
Intestinal Organoid Culture
Mini Review
Intestinal Organoid Culture
Efficient Establishment and Growth of Human Intestinal Organoid-Derived Monolayers
Scientific Poster
Efficient Establishment and Growth of Human Intestinal Organoid-Derived Monolayers
Dr. Bas Trietsch
Interview
Dr. Bas Trietsch
Proven Protocols for Intestinal Organoid Culture: Getting Started with IntestiCult™
E-Book
Proven Protocols for Intestinal Organoid Culture: Getting Started with IntestiCult™
Human Intestinal Organoid Course
On-Demand Training
Human Intestinal Organoid Course

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Legal Statement:

This product was developed under a license to intellectual property owned by Hubrecht Organoid Technology (HUB). This product is sold for research use only. Purchase of this product does not include the right to use it for drug screening aiming for commercial gain, equipment validation, biobanking, or for other commercial purposes. Purchasers wishing to use the product for purposes other than basic research use should contact HUB at www.huborganoids.nl to obtain a further license. Purchasers may apply for a License from HUB, which will not be unreasonably withheld by HUB.

Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.

Safety Statement:

CA WARNING: This product can expose you to Progesterone which is known to the State of California to cause cancer. For more information go to www.P65Warnings.ca.gov



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