IntestiCult™ OGM Human Basal Medium

Cell culture medium for establishment and maintenance of human colorectal cancer organoids

IntestiCult™ OGM Human Basal Medium

Cell culture medium for establishment and maintenance of human colorectal cancer organoids

From: 476 USD
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Cell culture medium for establishment and maintenance of human colorectal cancer organoids
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Product Advantages


  • Recapitulate tumor characteristics and heterogeneity with a physiologically relevant in vitro system

  • Predict patient response with highly representative tumoroids

  • Obtain consistent results with a complete medium formulation

  • Streamline your workflow with an easy-to-use format and optimized protocols

Overview

Efficiently grow Wnt-independent colorectal tumor organoids using IntestiCult™ OGM Human Basal Medium.

Tumoroids have high predictive value as an in vitro system as they retain the characteristics of the tumor of origin. Use IntestiCult™ OGM Human Basal Medium to provide optimal culture conditions for tumor organoid growth and expansion while maintaining tumor heterogeneity. The Wnt-free formulation ensures that Wnt-dependent, normal, healthy intestinal cells do not contaminate the tumoroid culture.

Tumor organoids generated using IntestiCult™ OGM Human Basal Medium are suitable for a variety of applications, including modeling tumor development and screening drugs for efficacy and toxicity.

For growing healthy intestinal organoids, please use IntestiCult™ Organoid Growth Medium (Human) or IntestiCult™-SF Organoid Growth Medium (Human). For differentiating human intestinal organoids, refer to IntestiCult™ Organoid Differentiation Medium (Human).

Learn how to culture human intestinal organoids in our On-Demand Intestinal Course or browse our Frequently Asked Questions (FAQs) about organoid workflows using IntestiCult™.
Cell Type
Cancer Cells and Cell Lines, Epithelial Cells, Intestinal Cells
Species
Human
Application
Organoid Culture
Brand
IntestiCult
Area of Interest
Cancer, Disease Modeling, Drug Discovery and Toxicity Testing, Epithelial Cell Biology, Organoids

Data Figures

Figure 1. Growth of Cancer-Derived Organoids in IntestiCult™ OGM Human Basal Medium

Organoids were established from colorectal cancer biopsies in published medium1, then switched to IntestiCult™ OGMH (P0), as described below. Cancer-derived organoids demonstrated efficient growth both after establishment in IntestiCult™ OGM Human Basal Medium, as well as after passaging. Data used with permission from Hubrecht Organoid Technology.

Figure 2. IntestiCult™ OGM Human Basal Medium Enables Organoid Growth Across Different Patients

Organoids were established in published medium1 from colorectal tumor biopsy samples, then switched to IntestiCult™ OGM Human Basal Medium after passaging as described above. Organoids were passaged twice in IntestiCult™ OGMH Basal Medium and imaged at the end of the second passage (day 6 - 12). Data used with permission from Hubrecht Organoid Technology.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
100-0190
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
100-0190
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.