IntestiCult™ OGM Human Basal Medium
Cell culture medium for establishment and maintenance of human colorectal cancer organoids
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Overview
Tumoroids have high predictive value as an in vitro system as they retain the characteristics of the tumor of origin. Use IntestiCult™ OGM Human Basal Medium to provide optimal culture conditions for tumor organoid growth and expansion while maintaining tumor heterogeneity. The Wnt-free formulation ensures that Wnt-dependent, normal, healthy intestinal cells do not contaminate the tumoroid culture.
Tumor organoids generated using IntestiCult™ OGM Human Basal Medium are suitable for a variety of applications, including modeling tumor development and screening drugs for efficacy and toxicity.
For growing healthy intestinal organoids, please use IntestiCult™ Organoid Growth Medium (Human) or IntestiCult™-SF Organoid Growth Medium (Human). For differentiating human intestinal organoids, refer to IntestiCult™ Organoid Differentiation Medium (Human).
Learn how to culture human intestinal organoids in our On-Demand Intestinal Course or browse our Frequently Asked Questions (FAQs) about organoid workflows using IntestiCult™.
Data Figures

Figure 1. Growth of Cancer-Derived Organoids in IntestiCult™ OGM Human Basal Medium
Organoids were established from colorectal cancer biopsies in published medium1, then switched to IntestiCult™ OGMH (P0), as described below. Cancer-derived organoids demonstrated efficient growth both after establishment in IntestiCult™ OGM Human Basal Medium, as well as after passaging. Data used with permission from Hubrecht Organoid Technology.

Figure 2. IntestiCult™ OGM Human Basal Medium Enables Organoid Growth Across Different Patients
Organoids were established in published medium1 from colorectal tumor biopsy samples, then switched to IntestiCult™ OGM Human Basal Medium after passaging as described above. Organoids were passaged twice in IntestiCult™ OGMH Basal Medium and imaged at the end of the second passage (day 6 - 12). Data used with permission from Hubrecht Organoid Technology.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Legal Statement:
This product was developed under a license to intellectual property owned by Hubrecht Organoid Technology (HUB). This product is sold for research use only. Purchase of this product does not include the right to use it for drug screening aiming for commercial gain, equipment validation, biobanking, or for other commercial purposes. Purchasers wishing to use the product for purposes other than basic research use should contact HUB at www.huborganoids.nl to obtain a further license. Purchasers may apply for a License from HUB, which will not be unreasonably withheld by HUB.
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.