Hygromycin B

Antibiotic for selecting transfected prokaryotic and eukaryotic cells

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Hygromycin B

Antibiotic for selecting transfected prokaryotic and eukaryotic cells

100 mg
Catalog #03813
127 USD

Overview

Hygromycin B, an aminoglycoside antibiotic, inhibits the growth of prokaryotic and eukaryotic cells. Specifically, it inhibits protein synthesis by interfering with translocation of the 70S ribosome subunit and inducing misreading of the mRNA template. Hygromycin B has been used to select transfectants in a wide variety of cells including bacteria, protozoans, yeast, fungi, plants, and mammalian cells. Resistance to Hygromycin B is conferred by a gene coding for a phosphotransferase that phosphorylates Hygromycin B, thereby inactivating it. Hygromycin B is an effective agent for the selection and maintenance of bacterial and eukaryotic cells stably transfected with vectors containing E. coli Hygromycin B resistance gene (hyg or hph).

This antibiotic is suitable for use in cell culture for the selection of hygromycin-resistant clones when using ClonaCell™-TCS Medium (Catalog #03814), and for the selection of any hygromycin-resistant transfected cell type.
Subtype:
Supplements
Cell Type:
CHO Cells; Hybridomas
Species:
Mouse
Application:
Cell Culture; Hybridoma Generation
Area of Interest:
Antibody Development; Cell Line Development; Drug Discovery and Toxicity Testing; Hybridoma Generation

Scientific Resources

Product Documentation

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Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.