EasySep™ Release Human CD4 Positive Selection Kit

Immunomagnetic positive selection kit using particle release technology

New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more

EasySep™ Release Human CD4 Positive Selection Kit

Immunomagnetic positive selection kit using particle release technology

From: 818 USD
Catalog #
17752_C
Immunomagnetic positive selection kit using particle release technology

Product Advantages

• Highly purified human CD4+ cells isolated in less than 30 minutes
• No-wash removal of EasySep™ Releasable RapidSpheres™

What's Included

  • EasySep™ Release Human CD4 Positive Selection Kit (Catalog #17752)
    • EasySep™ Release Human CD4 Positive Selection Cocktail, 1 mL
    • EasySep™ Releasable RapidSpheres™ 50201, 1 mL
    • EasySep™ Release Buffer (Concentrate), 3 x 1 mL
  • RoboSep™ Human CD4 Positive Selection Kit (Catalog #17752RF)
    • EasySep™ Release Human CD4 Positive Selection Cocktail, 1 mL
    • EasySep™ Releasable RapidSpheres™, 1 mL
    • EasySep™ Release Buffer (Concentrate), 3 x 1 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
    • EasySep™ EasyTube™-14 (Catalog #20128)
New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more

Overview

The EasySep™ Release Human CD4 Positive Selection Kit is designed to isolate CD4+ cells from fresh or previously frozen peripheral blood mononuclear cells or washed leukapheresis samples by immunomagnetic positive selection.

Desired cells are labeled with antibodies and magnetic particles, and separated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Then, bound magnetic particles are removed from the EasySep™-isolated CD4+ cells, which are immediately available for downstream applications. Following cell isolation with this EasySep™ Release kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins or other chemically related ligands. The CD4 antigen is strongly expressed on helper T cells and is expressed more weakly on peripheral blood monocytes and tissue macrophages.
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyPlate™ Magnet (Catalog #18102)
• EasyEights™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
T Cells, T Cells, CD4+
Species
Human
Sample Source
Leukapheresis, PBMC
Selection Method
Positive
Brand
EasySep, RoboSep
Area of Interest
Cell Therapy Research, Immunology

Scientific Resources

Product Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

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English
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Safety Data Sheet 1
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English
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Safety Data Sheet 2
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Safety Data Sheet 1
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English
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Safety Data Sheet 2
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17752
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English
Document Type
Safety Data Sheet 3
Catalog #
17752
Lot #
All
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English

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Starting with a single-cell suspension of human PBMCs, the CD4+ T cell content of the isolated fraction is typically 96.1 ± 4.1% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 24.0% and 98.1%, respectively.

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