EasySep™ Release Human CD3 Positive Selection Kit

Immunomagnetic positive selection kit using particle release technology
Catalog #
17751_C
Immunomagnetic positive selection kit using particle release technology
From: 750 USD
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Required Products
  1. EasySep™ Magnet
    EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer

Overview

Isolate highly purified and magnetic particle-free CD3+ cells from peripheral blood mononuclear cells or washed leukapheresis samples in as little as 30 minutes.

Using immunomagnetic positive selection, CD3+ cells are labeled with antibodies and magnetic particles, then separated without columns using an EasySep™ magnet. Unwanted cells are poured off, while desired cells remain in the tube. Bound magnetic particles are then removed from the isolated CD3+ cells, which are immediately available for downstream use. Following cell isolation with this EasySep™ Release kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins, or other chemically related ligands. The CD3 antigen is expressed on all T cells and CD56+ NKT cells.
For even higher efficiency, the process can be automated using RoboSep™.

This kit is designed for cell therapy research applications, but may be qualified for use as an ancillary material following the framework outlined in USP<1043>. STEMCELL can work with you to qualify this reagent as an ancillary material under an approved investigational new drug (IND) or clinical trial application (CTA). Learn more about how we can support your regulatory needs here.
Advantages
• Highly purified human CD3+ cells isolated in less than 30 minutes
• No-wash removal of EasySep™ Releasable RapidSpheres™
Components
  • EasySep™ Release Human CD3 Positive Selection Kit (Catalog #17751)
    • EasySep™ Release Human CD3 Positive Selection Cocktail, 1 mL
    • EasySep™ Releasable RapidSpheres™ 50201, 1 mL
    • EasySep™ Release Buffer (Concentrate), 3 x 1 mL
  • RoboSep™ Human CD3 Positive Selection Kit (Catalog #17751RF)
    • EasySep™ Release Human CD3 Positive Selection Cocktail, 1 mL
    • EasySep™ Releasable RapidSpheres™, 1 mL
    • EasySep™ Release Buffer (Concentrate), 3 x 1 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyPlate™ Magnet (Catalog #18102)
• EasyEights™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
T Cells
Species
Human
Sample Source
Leukapheresis, PBMC
Selection Method
Positive
Brand
EasySep, RoboSep
Area of Interest
Cell Therapy, Immunology

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
EasySep™ Release Human CD3 Positive Selection Kit
Catalog #
17751
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
RoboSep™ Release Human CD3 Positive Selection Kit
Catalog #
17751RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Release Human CD3 Positive Selection Kit
Catalog #
17751
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Release Human CD3 Positive Selection Kit
Catalog #
17751
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ Release Human CD3 Positive Selection Kit
Catalog #
17751RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ Release Human CD3 Positive Selection Kit
Catalog #
17751RF
Lot #
All
Language
English

Educational Materials (11)

Brochure
Tools For Your Immunology Research
Brochure
EasySep™ Release Free Your Positively Selected Cells from Magnetic Particles
Brochure
EasySep™ Cell Separation Technology
Brochure
T Cell Reagents for Your Cellular Therapy Research
Wallchart
Human Immune Cytokines
Wallchart
Frequencies of Cell Types in Human Peripheral Blood
Wallchart
Production of Chimeric Antigen Receptor T Cells
Video
Simultaneous Cell Isolation from Multiple Samples Using the EasyEights™ EasySep™ Magnet
0:57
Simultaneous Cell Isolation from Multiple Samples Using the EasyEights™ EasySep™ Magnet
Video
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
Video
CAR T Cell Manufacturing Workflow: Isolation, Activation and Expansion
0:59
CAR T Cell Manufacturing Workflow: Isolation, Activation and Expansion
Scientific Poster
Releasable RapidSpheres Enable Immunomagnetic Purification of Highly Viable and Functional Immune Cells from Complex Tissues in Less Than 30 Minutes

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Starting with a single-cell suspension of human PBMCs, the CD3+ cell content of the isolated fraction is typically 98.7 ± 0.9% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 38.4% and 99.0%, respectively.

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