EasySep™ Human TCR Alpha/Beta Depletion Kit
Immunomagnetic depletion kit
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EasySep™ Human TCR Alpha/Beta Depletion Kit -
EasySep™ Human TCR Alpha/Beta Depletion Kit -
Label for EasySep™ Human TCR Alpha/Beta Depletion Kit
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Required Products
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Magnet for column-free immunomagnetic separation -
Cell separation buffer
Overview
The EasySep™ Human TCR Alpha/Beta Depletion Kit is designed to deplete human T cell receptor alpha/beta+ (TCRab+) cells from leukapheresis samples or expanded TCRa/b knockout cell cultures. TCRab+ cells are targeted with antibody complexes and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Labeled cells remain in the tube while unlabeled cells are poured off.
Advantages:
⦁ Fast, easy-to-use, and column-free procedure
⦁ Untouched, viable cells
⦁ Untouched, viable cells
Components:
- EasySep™ Human TCR Alpha/Beta Depletion Kit (Catalog #17847)
- EasySep™ Human TCR Alpha/Beta Depletion Cocktail, 1 mL
- EasySep™ Dextran RapidSpheres™, 2 x 1 mL
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• Easy 50 EasySep™ Magnet (Catalog #18002)
Subtype:
Cell Isolation Kits
Cell Type:
T Cells
Species:
Human
Sample Source:
Leukapheresis; Other
Selection Method:
Depletion
Application:
Cell Isolation
Brand:
EasySep
Area of Interest:
Immunology; T Cell Engineering
Related Products
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Cas9 nuclease for the generation of double-strand breaks in CRISPR-Cas9 genome editing -
Human Peripheral Blood Leukapheresis Pack, Fresh
Primary human cells, fresh -
EasySep™ Human T Cell Isolation Kit
8-Minute cell isolation kit using immunomagnetic negative selection
Scientific Resources
Product Documentation
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Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages for
Workflow Stages
Data and Publications
Data
Figure 1. EasySep™ Human TCR Alpha/Beta Depletion Kit
In the above example, the frequencies of CD3+TCRαß+ cells in the starting and depleted fractions are 19.1% and 0.1%, respectively.
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.