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EasySep™ Human TCR Alpha/Beta Depletion Kit

Immunomagnetic depletion kit

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EasySep™ Human TCR Alpha/Beta Depletion Kit

Immunomagnetic depletion kit

From: 789 USD
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Immunomagnetic depletion kit
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Product Advantages


  • Fast, easy-to-use, and column-free procedure

  • Untouched, viable cells

What's Included

  • EasySep™ Human TCR Alpha/Beta Depletion Kit (Catalog #17847)
    • EasySep™ Human TCR Alpha/Beta Depletion Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™, 2 x 1 mL
    • EasySep™ Human TCR Alpha/Beta Depletion Kit (Catalog #100-1660)
      • EasySep™ Human TCR Alpha/Beta Depletion Cocktail, 10 mL
      • EasySep™ Dextran RapidSpheres™, 2 x 10 mL

      Overview

      Efficiently deplete human T cell receptor alpha/beta+ (TCRαß+) cells from leukapheresis samples or expanded TCRαß knockout cell culture samples by immunomagnetic positive selection, with the EasySep™ Human TCR Alpha/Beta Depletion Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

      This straightforward, optimized EasySep™ procedure involves labeling cells with antibody complexes recognizing TCRαß and magnetic particles. Labeled cells are separated from untouched cells using an EasySep™ magnet and by simply pouring or pipetting off the unlabeled cells. The TCRαß+ cells remain in the tube. Following magnetic cell isolation in as little as 13 minutes, desired cells are ready for downstream applications such as flow cytometry, culture, or cryopreservation.

      For large-scale depletion of human T cell receptor alpha/beta+ (TCRαß+) cells from leukapheresis samples, see the large-format (1x1010 cells) kit (Catalog #100-1660).

      Learn more about how immunomagnetic EasySep™ technology works. Explore additional products optimized for your workflow, including culture media, supplements, antibodies, and more.
      Magnet Compatibility
      • EasySep™ Magnet (Catalog #18000)
      • “The Big Easy” EasySep™ Magnet (Catalog #18001)
      • Easy 50 EasySep™ Magnet (Catalog #18002)
      • Easy 250 EasySep™ Magnet (Catalog #100-0821)
      Subtype
      Cell Isolation Kits
      Cell Type
      T Cells
      Species
      Human
      Sample Source
      Leukapheresis, Other
      Selection Method
      Depletion
      Application
      Cell Isolation
      Brand
      EasySep
      Area of Interest
      Immunology, Cell Therapy Development

      Data Figures

      The content of TCRab+ cells before and after the cell isolation procedure are 19.1% and 0.1%, respectively.

      Figure 1. Typical EasySep™ Human TCR Alpha/Beta Depletion Profile

      In the above example, the frequencies of CD3+TCRαß+ cells in the starting and depleted fractions are 19.1% and 0.1%, respectively.

      Residual TCRαβ+ Cells Can Be Removed from Expanded TRAC Knockout Cell Populations Using EasySep™ Human TCRαβ Depletion Kit

      Figure 2. EasySep™ Human TCRαβ Depletion Kit Can Be Used to Remove Residual TCRαβ+ Cells from Expanded TRAC Knockout Cell Populations After Genome Editing of Human Primary T Cells Using CRISPR-Cas9

      Gene-edited TCRαβ knockout T cells were expanded using ImmunoCult™-XF T Cell Expansion Medium (Catalog #10981) for 7 - 10 days, harvested, and resuspended in EasySep™ Buffer (Catalog # 20144) at 5 x 10⁷ cells/mL. Following a 13-minute EasySep™ depletion protocol using EasySep™ Human TCRαβ Depletion Kit (Catalog #17847), flow cytometry was performed to assess the depletion of TCRαβ+ cells. Representative data plots are shown for the frequency of TCRαβ cells in the expanded TRAC knockout cell population before and after EasySep™ depletion.

      Protocols and Documentation

      Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

      Document Type
      Product Name
      Catalog #
      Lot #
      Language
      Document Type
      Product Information Sheet
      Product Name
      EasySep™ Human TCR Alpha/Beta Depletion Kit
      Catalog #
      100-1660
      Lot #
      All
      Language
      English
      Document Type
      Product Information Sheet
      Product Name
      EasySep™ Human TCR Alpha/Beta Depletion Kit
      Catalog #
      17847
      Lot #
      All
      Language
      English
      Document Type
      Safety Data Sheet
      Product Name
      EasySep™ Human TCR Alpha/Beta Depletion Kit
      Catalog #
      100-1660
      Lot #
      All
      Language
      English
      Document Type
      Safety Data Sheet 1
      Product Name
      EasySep™ Human TCR Alpha/Beta Depletion Kit
      Catalog #
      17847
      Lot #
      All
      Language
      English
      Document Type
      Safety Data Sheet 2
      Product Name
      EasySep™ Human TCR Alpha/Beta Depletion Kit
      Catalog #
      17847
      Lot #
      All
      Language
      English

      Applications

      This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

      Research Area
      Workflow Stages

      Resources and Publications

      Educational Materials (8)

      Human Peripheral Blood Leukopaks Flyer
      Brochure
      Human Peripheral Blood Leukopaks Flyer
      EasySep™ Cell Separation Technology
      Brochure
      EasySep™ Cell Separation Technology
      ArciTect™ CRISPR-Cas9 Genome Editing System
      Brochure
      ArciTect™ CRISPR-Cas9 Genome Editing System
      Genome Editing of Human Primary T Cells Using CRISPR-Cas9
      Technical Bulletin
      Genome Editing of Human Primary T Cells Using CRISPR-Cas9
      Production of Chimeric Antigen Receptor T Cells
      Wallchart
      Production of Chimeric Antigen Receptor T Cells
      Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
      1:13
      Video
      Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
      How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
      1:57
      Video
      How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
      Optimization of Human T Cell Activation and Expansion Protocols Improves Efficiency of Genetic Modification and Overall Cell Yield
      Scientific Poster
      Optimization of Human T Cell Activation and Expansion Protocols Improves Efficiency of Genetic Modif...

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      Quality Statement:

      PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.

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