EasySep™ Human Plasmacytoid DC Isolation Kit

Immunomagnetic negative selection kit

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From: 982 USD

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Immunomagnetic negative selection kit
From: 982 USD

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Overview Related Products Scientific Resources Product Applications Data and Publications

Overview

The EasySep™ Human Plasmacytoid DC Isolation Kit is designed to isolate plasmacytoid dendritic cells (pDCs) from human peripheral blood mononuclear cells (PBMCs) or leukapheresis by immunomagnetic negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.

This product replaces the EasySep™ Human Plasmacytoid DC Enrichment Kit (Catalog #19062), for even faster cell isolations and is compatible with a greater number of EasySep™ magnets.
Advantages:
⦁ Fast, easy-to-use, and column-free
⦁ Up to 97% purity
⦁ Untouched, viable cells
Components:
  • EasySep™ Human Plasmacytoid DC Isolation Kit (Catalog #17977)
    • EasySep™ Human Plasmacytoid DC Isolation Cocktail Component A, 2 x 1 mL
    • EasySep™ Human Plasmacytoid DC Isolation Cocktail Component B, 2 x 1 mL
    • EasySep™ Dextran RapidSpheres™ 50103, 4 x 1 mL
    • Anti-Human CD32 (Fc gamma RII) Blocker, 2 x 0.8 mL
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• Easy 50 EasySep™ Magnet (Catalog #18002)
• RoboSep™-S (Catalog #21000)
Subtype:
Cell Isolation Kits
Cell Type:
Dendritic Cells
Species:
Human
Sample Source:
Leukapheresis; PBMC
Selection Method:
Negative
Application:
Cell Isolation
Brand:
EasySep; RoboSep
Area of Interest:
Immunology; Infectious Diseases

Scientific Resources

Product Documentation

Educational Materials

(5)
Brochure
EasySep™ Cell Separation Technology
Brochure
Cell Enrichment Prior to Cell Sorting
Wallchart
Frequencies of Cell Types in Human Peripheral Blood
Wallchart
Antigen Processing and Presentation
Video
1:57
Fast and Easy Cell Isolation with EasySep™

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area Workflow Stages for
Workflow Stages
Dendritic Cell Research
Cell Sourcing
Cell Isolation
Differentiation and Maturation
Cell Characterization

Data and Publications

Data

FACS Profile Results with EasySep™ Human Plasmacytoid DC Isolation Kit

Figure 1. Typical EasySep™ Human Plasmacytoid DC Isolation Profile

Starting with leukapheresis samples, the pDC content (Lin-HLA-DR+CD123+) of the isolated fraction is typically 90 ± 5.3% (mean ± SD using the silver “Big Easy” EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 0.2% and 89.8%, respectively.

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.