EasySep™ Human Plasmacytoid DC Isolation Kit

Immunomagnetic negative selection kit
Catalog #
17977_C
Immunomagnetic negative selection kit
From: 982 USD
Required Products
  1. EasySep™ Magnet
    EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer

Overview

The EasySep™ Human Plasmacytoid DC Isolation Kit is designed to isolate plasmacytoid dendritic cells (pDCs) from human peripheral blood mononuclear cells (PBMCs) or leukapheresis by immunomagnetic negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.

This product replaces the EasySep™ Human Plasmacytoid DC Enrichment Kit (Catalog #19062), for even faster cell isolations and is compatible with a greater number of EasySep™ magnets.
Advantages
⦁ Fast, easy-to-use, and column-free
⦁ Up to 97% purity
⦁ Untouched, viable cells
Components
  • EasySep™ Human Plasmacytoid DC Isolation Kit (Catalog #17977)
    • EasySep™ Human Plasmacytoid DC Isolation Cocktail Component A, 2 x 1 mL
    • EasySep™ Human Plasmacytoid DC Isolation Cocktail Component B, 2 x 1 mL
    • EasySep™ Dextran RapidSpheres™ 50103, 4 x 1 mL
    • Anti-Human CD32 (Fc gamma RII) Blocker, 2 x 0.8 mL
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• Easy 50 EasySep™ Magnet (Catalog #18002)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
Dendritic Cells
Species
Human
Sample Source
Leukapheresis, PBMC
Selection Method
Negative
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Immunology, Infectious Diseases

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
EasySep™ Human Plasmacytoid DC Isolation Kit
Catalog #
17977
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
RoboSep™ Human Plasmacytoid DC Isolation Kit
Catalog #
17977RF
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
EasySep™ Human Plasmacytoid DC Isolation Kit
Catalog #
17977
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
RoboSep™ Human Plasmacytoid DC Isolation Kit
Catalog #
17977RF
Lot #
All
Language
English

Educational Materials (5)

Brochure
EasySep™ Cell Separation Technology
Brochure
Cell Enrichment Prior to Cell Sorting
Wallchart
Frequencies of Cell Types in Human Peripheral Blood
Wallchart
Antigen Processing and Presentation
Video
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

FACS Profile Results with EasySep™ Human Plasmacytoid DC Isolation Kit

Figure 1. Typical EasySep™ Human Plasmacytoid DC Isolation Profile

Starting with leukapheresis samples, the pDC content (Lin-HLA-DR+CD123+) of the isolated fraction is typically 90 ± 5.3% (mean ± SD using the silver “Big Easy” EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 0.2% and 89.8%, respectively.

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