EasySep™ Human Neutrophil Isolation Kit
14-Minute cell isolation using immunomagnetic negative selection
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EasySep™ Human Neutrophil Isolation Kit -
EasySep™ Human Neutrophil Isolation Kit -
Label for EasySep™ Human Neutrophil Isolation Kit
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Required Products
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Cell separation buffer -
"The Big Easy" EasySep™ Magnet
Magnet for column-free immunomagnetic separation -
Compatible antibodies for purity assessment of isolated cells
Overview
The EasySep™ Human Neutrophil Isolation Kit is designed to isolate neutrophils from a polymorphonuclear cell-rich fraction of peripheral blood by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-neutrophils and magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube. The isolation kit is compatible with cells prepared using HetaSep™ (Catalog #07906) sedimentation or red blood cell lysis.
This product replaces the EasySep™ Human Neutrophil Enrichment Kit (Catalog #19257), for even faster cell isolations.
This product replaces the EasySep™ Human Neutrophil Enrichment Kit (Catalog #19257), for even faster cell isolations.
Advantages:
• Fast, easy-to-use and column-free
• Up to 99% purity
• Untouched, viable cells
• Up to 99% purity
• Untouched, viable cells
Components:
- EasySep™ Human Neutrophil Isolation Kit (Catalog #17957)
- EasySep™ Human Neutrophil Isolation Cocktail, 1 mL
- EasySep™ Dextran RapidSpheres™, 1 mL
- RoboSep™ Human Neutrophil Isolation Kit with Filter Tips (Catalog #17957RF)
- EasySep™ Human Neutrophil Isolation Cocktail, 1 mL
- EasySep™ Dextran RapidSpheres™, 1 mL
- RoboSep™ Buffer (Catalog #20104)
- RoboSep™ Filter Tips (Catalog #20125)
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• EasyPlate™ EasySep™ Magnet (Catalog #18102)
• RoboSep™-S (Catalog #21000)
Subtype:
Cell Isolation Kits
Cell Type:
Granulocytes and Subsets
Species:
Human
Sample Source:
PMNC; Whole Blood
Selection Method:
Negative
Application:
Cell Isolation
Brand:
EasySep; RoboSep
Area of Interest:
Immunology
Related Products
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Reagent for lysis of red blood cells -
For depletion of red blood cells from fresh blood samples and isolation of nucleated cells -
EasySep™ Human Pan-Granulocyte Isolation Kit
Immunomagnetic negative selection cell isolation kit
Labeling Antibodies
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Anti-Human CD16 Antibody, Clone 3G8
Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD16 -
Anti-Human CD66b Antibody, Clone G10F5
Mouse monoclonal IgM antibody against human, chimpanzee CD66b -
Anti-Human CD11b Antibody, Clone ICRF44
Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD11b
Scientific Resources
Product Documentation
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(10)
Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
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Workflow Stages for
Workflow Stages
Data and Publications
Data
Figure 1. Typical EasySep™ Human Neutrophil Isolation Profile
Starting with whole blood prepared using HetaSep™ or Lymphoprep™ with RBC lysis, the neutrophil content (CD45+CD16+CD66b+) of the isolated fraction typically ranges from 98.7 ± 0.9% (mean ± SD). In the above example, the purities of the start and final isolated fractions are 52.0% and 99.0%, respectively.
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.