EasySep™ Human Neutrophil Isolation Kit

14-Minute cell isolation using immunomagnetic negative selection

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From: 997 USD

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14-Minute cell isolation using immunomagnetic negative selection
From: 997 USD

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Overview Related Products Scientific Resources Product Applications Data and Publications

Overview

The EasySep™ Human Neutrophil Isolation Kit is designed to isolate neutrophils from a polymorphonuclear cell-rich fraction of peripheral blood by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-neutrophils and magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube. The isolation kit is compatible with cells prepared using HetaSep™ (Catalog #07906) sedimentation or red blood cell lysis.

This product replaces the EasySep™ Human Neutrophil Enrichment Kit (Catalog #19257), for even faster cell isolations.
Advantages:
• Fast, easy-to-use and column-free
• Up to 99% purity
• Untouched, viable cells
Components:
  • EasySep™ Human Neutrophil Isolation Kit (Catalog #17957)
    • EasySep™ Human Neutrophil Isolation Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™, 1 mL
  • RoboSep™ Human Neutrophil Isolation Kit with Filter Tips (Catalog #17957RF)
    • EasySep™ Human Neutrophil Isolation Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™, 1 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• EasyPlate™ EasySep™ Magnet (Catalog #18102)
• RoboSep™-S (Catalog #21000)
Subtype:
Cell Isolation Kits
Cell Type:
Granulocytes and Subsets
Species:
Human
Sample Source:
PMNC; Whole Blood
Selection Method:
Negative
Application:
Cell Isolation
Brand:
EasySep; RoboSep
Area of Interest:
Immunology

Scientific Resources

Product Documentation

Educational Materials

(10)
Brochure
EasySep™ Cell Separation Technology
Brochure
Tools For Your Immunology Research
Wallchart
Frequencies of Cell Types in Human Peripheral Blood
Wallchart
Human Immune Cytokines
Video
0:57
EasyEights™: Faster and Easier Multiple Sample Cell Isolations
Video
1:37
How to Isolate Mononuclear Cells from Whole Blood by Density Gradient Centrifugation
Video
1:41
RoboSep™-S Introduction
Video
1:13
The EasySep™ Pour-off: A Simple and Effective Way to Isolate Cells
Video
2:10
HetaSep™: A Guide to Isolating Nucleated Cells from Whole Blood
Video
1:57
Fast and Easy Cell Isolation with EasySep™

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area Workflow Stages for
Workflow Stages
Granulocyte Research
Cell Sourcing
Cell Isolation
Cell Characterization

Data and Publications

Data

Figure 1. Typical EasySep™ Human Neutrophil Isolation Profile

Starting with whole blood prepared using HetaSep™ or Lymphoprep™ with RBC lysis, the neutrophil content (CD45+CD16+CD66b+) of the isolated fraction typically ranges from 98.7 ± 0.9% (mean ± SD). In the above example, the purities of the start and final isolated fractions are 52.0% and 99.0%, respectively.

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.