EasySep™ HLA Chimerism Whole Blood Lymphoid Positive Selection Kit

Immunomagnetic positive selection cell isolation kit

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From: 546 USD

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Immunomagnetic positive selection cell isolation kit
From: 546 USD

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Overview

The EasySep™ Human Whole Blood Lymphoid Positive Selection Kit is designed to isolate CD3+ and CD19+ cells from whole blood or buffy coat by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD3, CD19 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD3 antigen is expressed on T lymphocytes and NKT cells while the CD19 antigen is expressed on B cells and follicular dendritic cells.

This product replaces the EasySep™ Human Whole Blood Lymphoid Positive Selection Kit (Catalog #18684) and EasySep™ HLA Whole Blood Lymphoid Positive Selection Kit (Catalog #18684HLA) for even faster cell isolations.
Advantages:
• Fast and easy-to-use
• Up to 99.9% purity
• No columns required
Components:
EasySep™ HLA Chimerism Whole Blood Lymphoid Positive Selection Kit (Catalog #17873)
    • EasySep™ HLA Chimerism Whole Blood Lymphoid Positive Selection Cocktail, 3 x 1 mL
    • EasySep™ Dextran RapidSpheres 50101, 3 x 1 mL
    • EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
RoboSep™ HLA Chimerism Whole Blood Lymphoid Positive Selection Kit (Catalog #17873RF)
    • EasySep™ HLA Chimerism Whole Blood Lymphoid Positive Selection Cocktail, 3 x 1 mL
    • EasySep™ Dextran RapidSpheres 50101, 3 x 1 mL
    • EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
    • RoboSep™ Buffer (Catalog #20104) x 2
    • RoboSep™ Filter Tips (Catalog #20125) x 2
Magnet Compatibility:
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• RoboSep™-S (Catalog #21000)
Subtype:
Cell Isolation Kits
Cell Type:
B Cells; Lymphocytes; T Cells
Species:
Human
Sample Source:
Buffy Coat; Whole Blood; Peripheral Blood
Selection Method:
Positive
Brand:
EasySep
Area of Interest:
Immunology

Scientific Resources

Educational Materials

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Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Starting with human whole blood, the lymphoid cell content (CD3+CD19+) of the isolated fraction is typically 99.5 ± 0.2% (gated on CD45; mean ± SD using “The Big Easy” EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 16.2% and 98.5%, respectively.

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.