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EasySep™ HLA Chimerism Whole Blood CD66b Positive Selection Kit

Immunomagnetic positive selection cell isolation kit.
Catalog #
17882_C
Immunomagnetic positive selection cell isolation kit.
From: 703 USD
New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more
Required Products
  1. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer

  2. "The Big Easy" EasySep™ Magnet
    "The Big Easy" EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

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Overview

The EasySep™ HLA Chimerism Whole Blood CD66b Positive Selection Kit is designed to isolate highly purified CD66b+ cells from fresh whole blood by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD66b and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD66b antigen is expressed on mature granulocytes. Isolated cells are immediately available for downstream applications such as flow cytometry, culture, or DNA/RNA extraction for lineage-specific chimerism analysis.

This product replaces the EasySep™ Human Whole Blood CD66b Positive Selection Kit (Catalog #18682) for even faster cell isolations.
Advantages
• Fast and easy-to-use
• Up to 99% purity
• No columns required
Components
EasySep™ HLA Chimerism Whole Blood CD66b Positive Selection Kit (Catalog #17882)
EasySep™ HLA Chimerism Whole Blood CD66b Positive Selection Cocktail, 3 x 1 mL
EasySep™ Dextran RapidSpheres™ 50102, 3 x 1 mL
EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
RoboSep™ HLA Chimerism Whole Blood CD66b Positive Selection Kit with Filter Tips (Catalog #17882RF)
EasySep™ HLA Chimerism Whole Blood CD66b Positive Selection Cocktail, 3 x 1 mL
EasySep™ Dextran RapidSpheres™ 50102, 3 x 1 mL
EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL
RoboSep™ Buffer (Catalog #20104) x 2
RoboSep™ Filter Tips (Catalog #20125) x2
Magnet Compatibility
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
Granulocytes and Subsets
Species
Human
Sample Source
Whole Blood
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Chimerism, Immunology

Related Products

Related Products

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet 1
Product Name
EasySep™ HLA Chimerism Whole Blood CD66b Positive Selection Kit
Catalog #
17882
Lot #
Lot 1000070129 and lower
Language
English
Document Type
Product Information Sheet 2
Product Name
EasySep™ HLA Chimerism Whole Blood CD66b Positive Selection Kit
Catalog #
17882
Lot #
Lot 1000070130 and higher
Language
English
Document Type
Product Information Sheet 1
Product Name
RoboSep™ HLA Chimerism Whole Blood CD66b Positive Selection Kit
Catalog #
17882RF
Lot #
Lot 1000070129 and lower
Language
English
Document Type
Product Information Sheet 2
Product Name
RoboSep™ HLA Chimerism Whole Blood CD66b Positive Selection Kit
Catalog #
17882RF
Lot #
Lot 1000070130 and higher
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ HLA Chimerism Whole Blood CD66b Positive Selection Kit
Catalog #
17882
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ HLA Chimerism Whole Blood CD66b Positive Selection Kit
Catalog #
17882
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
EasySep™ HLA Chimerism Whole Blood CD66b Positive Selection Kit
Catalog #
17882
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
EasySep™ HLA Chimerism Whole Blood CD66b Positive Selection Kit
Catalog #
17882
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ HLA Chimerism Whole Blood CD66b Positive Selection Kit
Catalog #
17882RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ HLA Chimerism Whole Blood CD66b Positive Selection Kit
Catalog #
17882RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ HLA Chimerism Whole Blood CD66b Positive Selection Kit
Catalog #
17882RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
RoboSep™ HLA Chimerism Whole Blood CD66b Positive Selection Kit
Catalog #
17882RF
Lot #
All
Language
English

Educational Materials (11)

Brochure
Cell Separation Products for HLA Analysis
Cell Separation Products for HLA Analysis
Brochure
EasySep™ Cell Separation Technology
EasySep™ Cell Separation Technology
Brochure
HLA Antibodies for Purity Assessment
HLA Antibodies for Purity Assessment
Technical Bulletin
Purity Assessment by Flow Cytometry for Lineage-Specific Chimerism Analysis
Purity Assessment by Flow Cytometry for Lineage-Specific Chimerism Analysis
Technical Bulletin
Fully Automated, High Purity Cell Isolation for Chimerism Labs
Fully Automated, High Purity Cell Isolation for Chimerism Labs
Wallchart
Frequencies of Cell Types in Human Peripheral Blood
Frequencies of Cell Types in Human Peripheral Blood
Wallchart
Human Immune Cytokines
Human Immune Cytokines
Video
Automate Cell Isolation with the RoboSep™-S Cell Separation Instrument
1:41
Automate Cell Isolation with the RoboSep™-S Cell Separation Instrument
Video
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
Webinar
Fully Automated Cell Separation for Chimerism Analysis with RoboSep™
56:02
Fully Automated Cell Separation for Chimerism Analysis with RoboSep™
Scientific Poster
Isolation of Very Pure Lymphoid (CD3+, CD19+ or CD56+) and Myeloid (CD15+ or CD33/CD66b+) Cell Subsets in as Little as 15 Minutes for Use in Lineage-Specific Chimerism Analysis
Isolation of Very Pure Lymphoid (CD3+, CD19+ or CD56+) and Myeloid (CD15+ or CD33/CD66b+) Cell Subsets in as Little as 15 Minutes for Use in Lineage-Specific Chimerism Analysis

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages
Workflow Stages for Granulocyte Research
Cell Sourcing
Cell Isolation
Cell Characterization
Workflow Stages for Myeloid Cell Research
Cell Sourcing
Cell Isolation
Cell Characterization
Workflow Stages for Chimerism Analysis
Cell Isolation
Characterization

Data and Publications

Data

Starting with whole blood, the CD66b+ cell content of the isolated fraction is typically 98.0 ± 0.8% (mean ± SD using “The Big Easy” EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 62.8% and 98.6%, respectively.

Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.

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